FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503566
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8085527.697880891118311No Hit
CAACGGAATCCCAAAAGCAGCTG6622106.304620735472125No Hit
AAGCTGCCAGTTGAAGAACTGT3520303.351528423775316No Hit
AACATTCAACGCTGTCGGTGAGT3469723.3033733495843225No Hit
TTCAAGTAATCCAGGATAGGCT3154263.003037254204905No Hit
TAGCAGCACGTAAATATTGGCG3128962.9789501965332534No Hit
CCCCCACTGCTAAATTTGACTG2408302.2928403553612173No Hit
TAGCTTATCAGACTGATGTTGAC1831661.7438458519706546No Hit
AACATTCAACGCTGTCGGTGAG1732311.6492589278726864No Hit
TAGCTTATCAGACTGATGTTGACT1389131.3225317953921554No Hit
TATTGCACTTGTCCCGGCCTGTAA1205231.1474483999053275No Hit
TAGCTTATCAGACTGATGTTGA1157901.1023875129646445No Hit
TGAGGTAGTAGGTTGTATAGTT1150981.0957992742655207No Hit
TGTAAACATCCTTGACTGGAAGCT1017150.9683854035857917No Hit
TATTGCACTTGTCCCGGCCTGTAT990580.94308923274248No Hit
GCCTGTCTGAGCGTCGCT933630.8888695515408767No Hit
CATAAAGTAGAAAGCACTACT847720.8070782817949638No Hit
TATTGCACTTGTCCCGGCCTGT835680.7956155081045808No Hit
CGCCTGTCTGAGCGTCGCT786640.7489266026414267No Hit
AGCAGCATTGTACAGGGCTATGA739430.7039799626146015No Hit
TGAGGTAGTAGATTGTATAGTT709310.6753039872363348No Hit
TGTAAACATCCCCGACTGGAAGCT567460.5402546144804535No Hit
TATTGCACTTGTCCCGGCCTGTA554700.5281063593069248No Hit
TTCAAGTAATTCAGGATAGGTT536220.5105123345728488No Hit
CCCATAAAGTAGAAAGCACTAC509220.48480677895488067No Hit
TTCTCACTACTGCACTTGACTAG496680.47286797645675765No Hit
ACTGGACTTGGAGTCAGAAGGC493490.4698309126633755No Hit
CAACGGAATCCCAAAAGCAGCTGT432180.411460260258278No Hit
CCCCCACTGCTAAATTTGACTGGCTT430040.4094228569611501No Hit
TGTCAGTTTGTCAAATACCCCAA382720.36437149059662216No Hit
TGTAAACATCCTACACTCTCAGCT333560.3175683382196104No Hit
AACATTCAACGCTGTCGGTGAGTT321120.3057247414830354No Hit
TGAGGTAGTAGTTTGTACAGTT316630.3014500027895288No Hit
CTTCTCACTACTGCACTTGACTAG307240.2925101817801688No Hit
CACCCGGCTGTGTGCACATGTGC304390.2897968175760499No Hit
TTCACAGTGGCTAAGTTCCG278890.2655193483813021No Hit
TACGCCTGTCTGAGCGTCGCT275530.2623204347932883No Hit
CATTGCACTTGTCTCGGTCTGA274880.26170159734322607No Hit
CCCATAAAGTAGAAAGCACT268120.25526568786257925No Hit
CTCCCACTGCTTCACTTGACTAG266920.25412321872400284No Hit
CCCCCCACTGCTAAATTTGACTGGCT256470.2441742166422337No Hit
GGAATACCGGGTGCTGTAGGCTTT255470.2432221590267534No Hit
CTACGCCTGTCTGAGCGTCGCT255160.2429270211659545No Hit
TCGTACGACTCTTAGCGG254270.24207968988817705No Hit
ACCGGGTGCTGTAGGCTT240290.22876992442376237No Hit
TGTCAGTTTGTCAAATACCCCA233850.2226386733800692No Hit
TAGCTTATCAGACTGATGTTGACA219990.2094431548295122No Hit
ACGCCTGTCTGAGCGTCGCT216650.20626328239380798No Hit
CAACACCAGTCGATGGGCTGT208600.19859921858919155No Hit
CAAAGAATTCTCCTTTTGGGCT206510.1966094181728377No Hit
TGAGGTAGTAGTTTGTGCTGTT195290.18592733172714868No Hit
GCGTTCGCGCTTTCCCCTG185030.17615922059232075No Hit
GGAATACCGGGTGCTGTAGGCTT181640.1729317452758425No Hit
TGTAAACATCCCCGACTGGAAGC180680.17201776996498142No Hit
TAGCAGCACGTAAATATTGGC167260.15924115676523573No Hit
TGTAAACATCCTACACTCAGCT166890.15888889544750803No Hit
TTCAAGTAATCCAGGATAGGC164210.1563373810380208No Hit
TTCAAGTAATCCAGGATAGGCTT163870.1560136814487575No Hit
CCCCCCACTGCTAAATTTGACTGG163590.15574710531642302No Hit
ACCGGGTGCTGTAGGCTTT162440.15465223905862066No Hit
CTCCCACTGCTTCACTTGACTAGCCT161940.15417621025088052No Hit
CAAAGAATTCTCCTTTTGGGCTT158380.15078688513977062No Hit
ACCGGGTGCTGTAGGCTTA158220.15063455592129377No Hit
TTCACAGTGGCTAAGTTCCGC157080.14954921023964624No Hit
AACGGAATCCCAAAAGCAGCTG156980.1494540044780982No Hit
TTATAAAGCAATGAGACTGATT155020.1475879715517568No Hit
TTCACAGTGGCTAAGTTCTGC149750.1425706279181756No Hit
AGCTCGGTCTGAGGCCCCTCAGT149010.14186610528272017No Hit
TATTGCACTTGTCCCGGCCTGTT147920.14082836248184666No Hit
TAGCAGCACGTAAATATTGGCGT147780.1406950744156794No Hit
CAACGGAATCCCAAAAGCAGCT147240.14018096330332003No Hit
TGAGGTAGTAGGTTGTGTGGTT143310.13643937687448243No Hit
ATCCCGGACGAGCCCCCA142390.13556348386824055No Hit
AACCGGGCGGAAACACCA141710.13491608468971394No Hit
ACCCCCCACTGCTAAATTTGACTG141340.13456382337198625No Hit
GACCCGCGGGCGCTCTCCAGTCCT138760.13210751472404705No Hit
GTCAGGATGGCCGAGCGGTCTAAGG134540.12808983158672016No Hit
ACTGGACTTGGAGTCAGAAGGCA134180.12774709084514727No Hit
TATTGCACTTGTCCCGGCCTGTAG131680.1253669468064465No Hit
TGATGTAGTAGGTTGTATAGTT128330.12217755379458747No Hit
AGCAGCATTGTACAGGGCTATGT128100.12195858054302701No Hit
ACTGGACTTGGAGTCAGAAGG127260.12115885214602354No Hit
CAAAGTGCTGTTCGTGCAGGT124860.11887391386887082No Hit
ATCACATTGCCAGGGATTTCCA124810.1188263109880968No Hit
ACCACAGGGTAGAACCACGGAC124150.11819795296187982No Hit
AGCAGCATTGTACAGGGCTATG123620.11769336242567524No Hit
CAAAGTGCTGTTCGTGCAGGTAG123190.11728397765101871No Hit
TTCACAGTGGCTAAGTTCTG121320.11550362991007054No Hit
TCCTGTCTGAGCGTCGCT116490.11090519162730067No Hit
TGAGGTAGTAGGTTGTATAGT115040.10952470808485422No Hit
TCGTTTCCCGGCCAATGCACCA114000.10853456816475471No Hit
GGGCTACGCCTGTCTGAGCGTCGCT107480.10232715251182313No Hit
ATCACATTGCCAGGGATTTCC105140.1000993376915992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)