FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7094979
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3369774.74951370539645No Hit
CCCCCCACTGCTAAATTTGACTG3164744.460534696438144No Hit
AAGCTGCCAGTTGAAGAACTGT3001934.231062558465641No Hit
CGCCTGTCTGAGCGTCGCT2579163.6351904635658543No Hit
TAGCAGCACGTAAATATTGGCG2292203.2307354257144385No Hit
AACATTCAACGCTGTCGGTGAGT2176943.068282513591654No Hit
TTCAAGTAATCCAGGATAGGCT2014822.8397828943538803No Hit
TAGCTTATCAGACTGATGTTGAC1570752.2138895689472795No Hit
GCCTGTCTGAGCGTCGCT1366641.9262072516352762No Hit
CCCCCACTGCTAAATTTGACTG1356771.9122960053863443No Hit
AACATTCAACGCTGTCGGTGAG1175571.6569041289621858No Hit
TAGCTTATCAGACTGATGTTGACT951631.3412724688825717No Hit
TAGCTTATCAGACTGATGTTGA881711.2427239037634925No Hit
TGAGGTAGTAGGTTGTATAGTT859311.2111522810708812No Hit
CATAAAGTAGAAAGCACTACT842031.1867970292794383No Hit
TATTGCACTTGTCCCGGCCTGT690580.973336214243904No Hit
TGTAAACATCCTTGACTGGAAGCT651810.9186919369317372No Hit
TATTGCACTTGTCCCGGCCTGTAA591850.8341814683313369No Hit
AGCAGCATTGTACAGGGCTATGA590100.8317149353084765No Hit
TGAGGTAGTAGATTGTATAGTT545870.7693750749649858No Hit
TCCTGTCTGAGCGTCGCT540150.7613130355988369No Hit
TATTGCACTTGTCCCGGCCTGTAT530010.7470212385406637No Hit
TACGCCTGTCTGAGCGTCGCT470430.6630463599680845No Hit
ACTGGACTTGGAGTCAGAAGGC464880.6552239266670133No Hit
GCGTTCGCGCTTTCCCCTG442750.6240328547836435No Hit
AACCGGGCGGAAACACCA420480.5926444602584447No Hit
TTCAAGTAATTCAGGATAGGTT417490.5884302124079578No Hit
CAACACCAGTCGATGGGCTGT395670.5576760692314946No Hit
TATTGCACTTGTCCCGGCCTGTA358750.5056392696863514No Hit
ACGCCTGTCTGAGCGTCGCT348890.491742117911836No Hit
CATTGCACTTGTCTCGGTCTGA328880.46353907460473104No Hit
TGTAAACATCCCCGACTGGAAGCT285870.40291874014003426No Hit
TGTCAGTTTGTCAAATACCCCAA285270.4020730716750536No Hit
TGAGGTAGTAGTTTGTACAGTT267240.37666073430238484No Hit
ACCGGGTGCTGTAGGCTT252380.3557163453196972No Hit
CCCATAAAGTAGAAAGCACT250400.35292563938526106No Hit
TGTAAACATCCTACACTCTCAGCT231170.32582196508263095No Hit
TCGTACGACTCTTAGCGG227490.3206351984974163No Hit
CTACGCCTGTCTGAGCGTCGCT215350.3035245065559743No Hit
TTATAAAGCAATGAGACTGATT211120.2975625438778607No Hit
CAACGGAATCCCAAAAGCAGCTGT201870.2845251550427422No Hit
TCGTTCGCGCTTTCCCCTG193170.27226296230052266No Hit
AACATTCAACGCTGTCGGTGAGTT185630.2616357285905991No Hit
CCCATAAAGTAGAAAGCACTAC184290.25974706901880895No Hit
TGTAAACATCCTACACTCAGCT181390.25565967143806906No Hit
TGTCAGTTTGTCAAATACCCCA171550.24179070861238633No Hit
TGAGGTAGTAGTTTGTGCTGTT159210.22439812718261742No Hit
CACCCGGCTGTGTGCACATGTGC159030.22414442664312326No Hit
ATCCCGGACGAGCCCCCA154480.2177314407836866No Hit
TTCTCACTACTGCACTTGACTAG154030.2170971894349511No Hit
ACCACAGGGTAGAACCACGGAC152560.2150253016957485No Hit
CCTCGACACAAGGGTTTGT151540.21358766530528137No Hit
TTCACAGTGGCTAAGTTCCG149730.21103656543592308No Hit
ATCCCACCGCTGCCACCA145280.20476452432064987No Hit
TACCTGGTTGATCCTGCC136920.19298154370858603No Hit
TAGCAGCACGTAAATATTGGC133310.18789343844428574No Hit
TTCAAGTAATCCAGGATAGGCTT131760.18570879490975237No Hit
ACCGGGTGCTGTAGGCTTT127190.17926762010148303No Hit
TAGCTTATCAGACTGATGTTGACA125850.1773789605296929No Hit
AGCAGCATTGTACAGGGCTATGT123650.17427817615809715No Hit
AACGGAATCCCAAAAGCAGCTG121690.17151565917249367No Hit
CAACGGAATCCCAAAAGCAGCT121680.17150156469807734No Hit
AGCAGCATTGTACAGGGCTATG121310.1709800691446726No Hit
CTCGACACAAGGGTTTGT117460.16555369649438004No Hit
TAGCAGCACGTAAATATTGGCGT116250.1638482650900024No Hit
TGAGGTAGTAGGTTGTGTGGTT112150.15806953057930123No Hit
TTCACAGTGGCTAAGTTCCGC110440.15565937545410635No Hit
CTTCTCACTACTGCACTTGACTAG110200.15532110806811408No Hit
ACTGGACTTGGAGTCAGAAGG109730.1546586677705459No Hit
CCTGTCTGAGCGTCGCTT109230.15395394404972867No Hit
ACCGGGTGCTGTAGGCTTA107730.15183977288727704No Hit
TTCAAGTAATCCAGGATAGGC103860.1463852112881518No Hit
TCAGTGCACTACAGAACTTTGT103600.14601875495332683No Hit
ACTGGACTTGGAGTCAGAAGGCA103070.1452717478092606No Hit
TAGTGGGTTATCAGAACT101880.14359450535371562No Hit
AGAGGTAGTAGGTTGCATAGTT93860.1322907368718075No Hit
CTGCGTTCGCGCTTTCCCCTG93230.13140278498357782No Hit
TGAGGTAGTAGGTTGTATAGT92070.1297678259512819No Hit
TATTGCACTTGTCCCGGCCTGTT91760.12933089724437521No Hit
CAACACCAGTCGATGGGCTGTC91110.12841475640731284No Hit
CAAGTCCTTCTGATCGAGGCCC90870.12807648902132057No Hit
ACTGGACTTGGAGTCAGAAGGCT90800.12797782770040617No Hit
CACTAGATTGTGAGCTCCTGGA89300.1258636565379545No Hit
TGTAAACATCCCCGACTGGAAGC88690.12500389359855751No Hit
AATGACACGATCACTCCCGTTG88430.12463743726373257No Hit
CTCCCACTGCTTCACTTGACTAG83120.11715327134865375No Hit
TAGCAGCACATAATGGTTTGT83080.11709689345098837No Hit
TTCACAGTGGCTAAGTTCTG82070.11567335153493759No Hit
CAAAGTGCTGTTCGTGCAGGT81280.1145598880560464No Hit
CCAAGTCCTTCTGATCGAGGCCC81030.11420752619563779No Hit
TTCACAGTGGCTAAGTTCTGC80890.11401020355380896No Hit
AACATTCAACGCTGTCGGTGA80480.11343233010273886No Hit
CATAAAGTAGAAAGCACTAC79970.1127135119075053No Hit
CAAAGTGCTGTTCGTGCAGGTAG79080.11145910368445064No Hit
TCAGTGCACTACAGAACTTTGTC75930.1070193442433022No Hit
TCCTTCTGATCGAGGCCC75590.10654013211314649No Hit
TCGTTTCCCGGCCAATGCACCA74610.10515887362034476No Hit
TGTCAGTTTGTCAAATACCCC72670.10242454558357396No Hit
ATAGGCGATAGAAATTGAAACCT72540.10224131741616148No Hit
CAAAGAATTCTCCTTTTGGGCTT71590.10090234234660879No Hit
TAATGTGAATTGCAGGACA71280.1004654136397021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)