FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7398718
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7004799.4675726254197No Hit
CCCCCCACTGCTAAATTTGACTG5436137.347394508075588No Hit
AAGCTGCCAGTTGAAGAACTGT3730665.042305977873464No Hit
AACATTCAACGCTGTCGGTGAGT2588623.498741268419745No Hit
TTCAAGTAATCCAGGATAGGCT2427153.2805007570230407No Hit
TAGCAGCACGTAAATATTGGCG2178702.944699338452959No Hit
CCCCCACTGCTAAATTTGACTG1826042.468049194468555No Hit
AACATTCAACGCTGTCGGTGAG1364561.8443195158945103No Hit
TAGCTTATCAGACTGATGTTGAC1145921.5488088612108204No Hit
TATTGCACTTGTCCCGGCCTGTAA992721.3417459619355678No Hit
TAGCTTATCAGACTGATGTTGACT925691.2511491855751227No Hit
TATTGCACTTGTCCCGGCCTGTAT872371.1790826464801065No Hit
TGTAAACATCCTTGACTGGAAGCT793731.0727939624134883No Hit
TGAGGTAGTAGGTTGTATAGTT768491.0386799442822392No Hit
TATTGCACTTGTCCCGGCCTGT728830.9850760631774316No Hit
AGCAGCATTGTACAGGGCTATGA637570.8617303700451889No Hit
TAGCTTATCAGACTGATGTTGA587100.7935158496377345No Hit
TGTAAACATCCCCGACTGGAAGCT502700.6794420330657285No Hit
CAACGGAATCCCAAAAGCAGCTGT477510.6453955942096996No Hit
TATTGCACTTGTCCCGGCCTGTA471680.637515850719003No Hit
TGAGGTAGTAGATTGTATAGTT439380.5938596389266357No Hit
CGCCTGTCTGAGCGTCGCT420180.5679091972420087No Hit
CATAAAGTAGAAAGCACTACT416830.5633813858022431No Hit
TTAATGCTAATCGTGATAGGGGTT410760.5551772617904887No Hit
ACTGGACTTGGAGTCAGAAGGC379910.5134808489795124No Hit
TTCAAGTAATTCAGGATAGGTT359270.4855841241685384No Hit
CCCATAAAGTAGAAAGCACTAC347510.46968947863670435No Hit
TGTAAACATCCTACACTCTCAGCT279280.37747079967097No Hit
GGAATACCGGGTGCTGTAGGCTTT260890.35261514224491325No Hit
CTACGCCTGTCTGAGCGTCGCT253210.3422349655710625No Hit
TTCTCACTACTGCACTTGACTAG242280.32746213600788676No Hit
AACATTCAACGCTGTCGGTGAGTT235330.31806861675225356No Hit
CATTGCACTTGTCTCGGTCTGA224460.30337688231934234No Hit
CACCCGGCTGTGTGCACATGTGC223500.302079360235111No Hit
CCCCCACTGCTAAATTTGACTGGCTT221990.30003846612345547No Hit
TGTCAGTTTGTCAAATACCCCAA214610.2900637651009269No Hit
GGAATACCGGGTGCTGTAGGCTT203130.27454756351032705No Hit
CCCATAAAGTAGAAAGCACT202810.2741150561489166No Hit
TGAGGTAGTAGTTTGTACAGTT198050.2676815091479362No Hit
AACCGGGCGGAAACACCA197040.2663164077884844No Hit
TACGCCTGTCTGAGCGTCGCT195630.2644106722272696No Hit
TCGTACGACTCTTAGCGG183500.24801594005880478No Hit
TGATGTAGTAGGTTGTATAGTT174120.23533806802746096No Hit
TGTCAGTTTGTCAAATACCCCA156280.2112257826288284No Hit
CTTCTCACTACTGCACTTGACTAG154900.20936059463274584No Hit
CCCCCCACTGCTAAATTTGACTGG152420.20600866258181486No Hit
CAAAGAATTCTCCTTTTGGGCT151980.2054139649598755No Hit
TAGCTTATCAGACTGATGTTGACA151490.2047516880627157No Hit
CTCCCACTGCTTCACTTGACTAG151150.20429214899121712No Hit
CAACGGAATCCCAAAAGCAGCT148500.20071044740453686No Hit
AGCTCGGTCTGAGGCCCCTCAGT146540.19806133981589785No Hit
GCCTGTCTGAGCGTCGCT146250.19766938001961962No Hit
CCCCCCACTGCTAAATTTGACTGGCT143320.19370923449170518No Hit
TGTAAACATCCCCGACTGGAAGC142110.19207381603137194No Hit
TTCAAGTAATCCAGGATAGGCTT141760.19160076110482924No Hit
TGAGGTAGTAGTTTGTGCTGTT141330.19101957933793395No Hit
AACGGAATCCCAAAAGCAGCTG140320.18965447797848223No Hit
TAGCAGCACGTAAATATTGGC139760.18889759009601395No Hit
GCGTTCGCGCTTTCCCCTG138700.1874649094613418No Hit
CAACACCAGTCGATGGGCTGT137770.1862079349422427No Hit
ACGCCTGTCTGAGCGTCGCT134130.18128816370619882No Hit
GGGCTACGCCTGTCTGAGCGTCGCT131980.17838225487172238No Hit
TATTGCACTTGTCCCGGCCTGTT124310.16801559405291566No Hit
TGTAAACATCCTACACTCAGCT124280.1679750464877834No Hit
TGAGAACTGAATTCCATAGGCTGT123800.16732628544566774No Hit
CAAAGAATTCTCCTTTTGGGCTT121800.16462311443685246No Hit
TCGTTTCCCGGCCAATGCACCA120070.16228487151422719No Hit
AGCAGCATTGTACAGGGCTATGT117320.15856801137710616No Hit
ACTGGACTTGGAGTCAGAAGGCA116520.15748674297358No Hit
ACCACAGGGTAGAACCACGGAC115470.15606757819395198No Hit
TAGCAGCACGTAAATATTGGCGT114960.15537826958670406No Hit
TGAGGTAGTAGGTTGTGTGGTT113810.15382394625663526No Hit
ACCCCCCACTGCTAAATTTGACTG112790.15244532904213948No Hit
TATTGCACTTGTCCCGGCCTGTAG111110.1501746653947346No Hit
TGAGAACTGAATTCCATGGGTT109930.14857979449953357No Hit
CACTAGATTGTGAGCTCCTGGA102320.13829422881099132No Hit
TGATGTAGTAGATTGTATAGTT100000.13515855044076555No Hit
CCCCCCACTGCTAAATTTGACTGTA97250.1314416903036445No Hit
ACTGGACTTGGAGTCAGAAGGCT96020.1297792401332231No Hit
CAAAGTGCTGTTCGTGCAGGTAG94710.12800866312244905No Hit
CACTAGATTGTGAGCTCCTGGAA94380.12756263990599453No Hit
TTCAAGTAATCCAGGATAGGC93960.1269949739941433No Hit
TCGTTTCCCGGGCGGCGCACCA93950.12698145813909925No Hit
TTCACAGTGGCTAAGTTCCG93770.12673817274830584No Hit
ATCACATTGCCAGGGATTTCC91920.1242377395651517No Hit
AGCAGCATTGTACAGGGCTATG91660.1238863273340057No Hit
ATCCCGGACGAGCCCCCA86750.11725004250736412No Hit
TTCACAGTGGCTAAGTTCCGC86640.11710136810187927No Hit
ACTTGACTTGGAGTCAGAAGGC86070.1163309643643669No Hit
ATCACATTGCCAGGGATTTCCA85420.11545243378650193No Hit
CTCCCACTGCTTCACTTGACTAGCCT84660.1144252288031521No Hit
TAGCAGCACATCATGGTTTACA84120.11369537263077197No Hit
GACCCGCGGGCGCTCTCCAGTCCT83960.11347911895006675No Hit
TTATAAAGCAATGAGACTGATT79060.10685634997846924No Hit
GAATACCGGGTGCTGTAGGCTTT78840.10655900116749956No Hit
ACCGGGTGCTGTAGGCTT78410.10597781940060427No Hit
TATTGCACTTGTCCCGGCCTGTAAT77960.10536960592362082No Hit
GTCAGGATGGCCGAGCGGTCTAAGG77900.10528851079335637No Hit
TTCACCACCTTCTCCACCCAGC76950.10400450456416908No Hit
TTCACAGTGGCTAAGTTCTGC76550.10346387036240604No Hit
AACATTCAACGCTGTCGGTGAGA75330.1018149360470287No Hit
CCCCCCACTGCTAAATTTGACTGT74170.10024709686191581No Hit
CCAAGTCCTTCTGATCGAGGCCC74030.10005787489129875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)