FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8881309
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7516898.463718580222803No Hit
CAACGGAATCCCAAAAGCAGCTG6795167.6510793622877No Hit
AAGCTGCCAGTTGAAGAACTGT3469263.9062485045841777No Hit
TAGCAGCACGTAAATATTGGCG3262053.672938302225494No Hit
TTCAAGTAATCCAGGATAGGCT3027783.4091596182499675No Hit
TAGCTTATCAGACTGATGTTGAC2554742.8765354296309247No Hit
CCCCCACTGCTAAATTTGACTG2374272.6733334016415826No Hit
AACATTCAACGCTGTCGGTGAGT2274202.560658569586983No Hit
TAGCTTATCAGACTGATGTTGACT2091642.3551032848873965No Hit
TGTAAACATCCTTGACTGGAAGCT1665591.8753879636436475No Hit
AACATTCAACGCTGTCGGTGAG1456061.6394655337405781No Hit
TATTGCACTTGTCCCGGCCTGTAA1174001.3218772142710045No Hit
TAGCTTATCAGACTGATGTTGA1169801.3171481816475477No Hit
TATTGCACTTGTCCCGGCCTGTAT996641.1221769223433167No Hit
TTAATGCTAATCGTGATAGGGGTT805210.9066343711270489No Hit
CATAAAGTAGAAAGCACTACT740770.8340774991614412No Hit
TGTAAACATCCCCGACTGGAAGCT703130.7916963591740812No Hit
AGCAGCATTGTACAGGGCTATGA675550.7606423782800487No Hit
TGAGGTAGTAGGTTGTATAGTT658890.7418838822070035No Hit
TGTAAACATCCTACACTCTCAGCT605640.6819265043024626No Hit
TTCAAGTAATTCAGGATAGGTT574600.6469767012948203No Hit
TATTGCACTTGTCCCGGCCTGTA518420.5837202601553442No Hit
TGAGGTAGTAGATTGTATAGTT513830.5785521030739951No Hit
TATTGCACTTGTCCCGGCCTGT492580.5546254499196008No Hit
CAACGGAATCCCAAAAGCAGCTGT480280.5407761400937632No Hit
TGTCAGTTTGTCAAATACCCCAA461230.5193265992659415No Hit
CAAAGAATTCTCCTTTTGGGCT455840.5132576740658387No Hit
CCCATAAAGTAGAAAGCACTAC455130.5124582423604448No Hit
TTCTCACTACTGCACTTGACTAG406490.45769154074022195No Hit
TAGCTTATCAGACTGATGTTGACA378340.42599576256157734No Hit
TGTCAGTTTGTCAAATACCCCA377150.4246558699849313No Hit
CCCCCACTGCTAAATTTGACTGGCTT352510.39691221192731835No Hit
CTTCTCACTACTGCACTTGACTAG281530.3169915605908994No Hit
ACTGGACTTGGAGTCAGAAGGC268790.30264682829974726No Hit
AACATTCAACGCTGTCGGTGAGTT264980.2983569201341829No Hit
AACCGGGCGGAAACACCA241480.271896856645794No Hit
TGTAAACATCCTACACTCAGCT235210.26483708651506216No Hit
CAAAGAATTCTCCTTTTGGGCTT231190.260310726718325No Hit
TGAGGTAGTAGTTTGTACAGTT220320.24807153990475955No Hit
TTCACAGTGGCTAAGTTCCG215520.24266693119223753No Hit
TAGCAGCACGTAAATATTGGC214160.2411356253903563No Hit
CCCATAAAGTAGAAAGCACT211310.23792663896729638No Hit
CATTGCACTTGTCTCGGTCTGA210770.23731862048713767No Hit
TGATGTAGTAGGTTGTATAGTT206630.23265714547258745No Hit
CCCCCCACTGCTAAATTTGACTGG198680.22370576229247288No Hit
CCCCCCACTGCTAAATTTGACTGGCT194880.21942711372839296No Hit
TGTAAACATCCCCGACTGGAAGC182770.20579173633075934No Hit
GGAATACCGGGTGCTGTAGGCTTT178660.20116404012066239No Hit
TGAGAACTGAATTCCATAGGCTGT177120.19943006149206158No Hit
ATCACATTGCCAGGGATTTCCA174770.1967840551432227No Hit
ACCCCCCACTGCTAAATTTGACTG168260.18945405457686473No Hit
TGATGTAGTAGATTGTATAGTT161170.1814709971244104No Hit
TTCAAGTAATCCAGGATAGGCTT158080.17799178026572435No Hit
CAACGGAATCCCAAAAGCAGCT156120.17578489837477784No Hit
ATCACATTGCCAGGGATTTCC149230.1680270329520119No Hit
TAGCAGCACGTAAATATTGGCGT148060.16670965957833467No Hit
CACCCGGCTGTGTGCACATGTGC146030.16442396047699726No Hit
CCCCCCACTGCTAAATTTGACTGTA144430.16262242423948992No Hit
TAGCAGCACATCATGGTTTACA141760.15961611064314957No Hit
TATTGCACTTGTCCCGGCCTGTAG135430.15248878290351117No Hit
CACTAGATTGTGAGCTCCTGGAA132920.14966262293092156No Hit
TTCAAGTAATCCAGGATAGGC125810.14165704627549836No Hit
TATTGCACTTGTCCCGGCCTGTT123880.13948394318900514No Hit
GGAATACCGGGTGCTGTAGGCTT123030.13852687706282937No Hit
TGTCAGTTTGTCAAATACCCC122290.13769366655298224No Hit
TGAGAACTGAATTCCATGGGTT119520.13457475694179766No Hit
CTCCCACTGCTTCACTTGACTAG119220.13423696889726502No Hit
TCGTTTCCCGGCCAATGCACCA116090.1307127136326413No Hit
TTAATGCTAATCGTGATAGGGGTTT114850.12931652304857313No Hit
TGAGGTAGTAGTTTGTGCTGTT114790.12924896543966663No Hit
AGCAGCATTGTACAGGGCTATGT114420.12883236018474303No Hit
TGTCAGTTTGTCAAATACC114060.1284270145313039No Hit
AGCTCGGTCTGAGGCCCCTCAGT113580.1278865536600517No Hit
TGTAAACATCCTTGACTGGAAGC108340.12198652248221518No Hit
CCCATAAAGTAGAAAGCACTA108050.12165999403916698No Hit
TTATAAAGCAATGAGACTGATT107260.12077048552189772No Hit
ACTGGACTTGGAGTCAGAAGGCA107040.12052277428924046No Hit
AACGGAATCCCAAAAGCAGCTG106620.1200498710268948No Hit
CACTAGATTGTGAGCTCCTGGA105770.11909280490071902No Hit
CCCCCCACTGCTAAATTTGACTGT104400.11755023949735338No Hit
CAAAGTGCTGTTCGTGCAGGTAG104010.11711111503946096No Hit
ATGCACCTGGGCAAGGATTCTGA103590.1166382117771153No Hit
CAACACCAGTCGATGGGCTGT103490.11652561576227108No Hit
TATTGCACTTGTCCCGGCCTGTAAT102890.11585003967320583No Hit
CTACGCCTGTCTGAGCGTCGCT100230.1128549856783499No Hit
CAGTGCAATAGTATTGTCAAAGC99100.11158265071061033No Hit
TATTGCACTTGTCCCGGCCTGTAAA98890.11134619907943749No Hit
CCCCCACTGCTAAATTTGACTGGCTA98290.11067062299037227No Hit
AGCTACATTGTCTGCTGGGTTT97070.10929695160927291No Hit
CTCCCACTGCTTCACTTGACTAGCCT95030.10699999290645107No Hit
GCCTGTCTGAGCGTCGCT94740.10667346446340287No Hit
CGCCTGTCTGAGCGTCGCT94660.1065833876515275No Hit
ACTTGACTTGGAGTCAGAAGGC93520.10529979308230351No Hit
AGCAGCATTGTACAGGGCTATG88900.10009785719650109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)