FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8991640
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7352868.177440377951074No Hit
CCCCCCACTGCTAAATTTGACTG6670797.418880204278641No Hit
TTCAAGTAATCCAGGATAGGCT3298123.6679849282222157No Hit
AAGCTGCCAGTTGAAGAACTGT3029123.3688181466339846No Hit
TAGCAGCACGTAAATATTGGCG2922453.250185728076302No Hit
AACATTCAACGCTGTCGGTGAGT2771333.082118501185546No Hit
CCCCCACTGCTAAATTTGACTG2750383.058819080835087No Hit
TAGCTTATCAGACTGATGTTGAC1832342.0378262474921147No Hit
TAGCTTATCAGACTGATGTTGACT1703531.8945709570223006No Hit
AACATTCAACGCTGTCGGTGAG1667481.8544781597128No Hit
TGAGGTAGTAGGTTGTATAGTT1315941.4635149983762694No Hit
TGTAAACATCCTTGACTGGAAGCT1128481.2550324523668652No Hit
TAGCTTATCAGACTGATGTTGA1119791.245367919534145No Hit
TATTGCACTTGTCCCGGCCTGTAA1029081.1444853219212512No Hit
CATAAAGTAGAAAGCACTACT875490.9736710989318967No Hit
TATTGCACTTGTCCCGGCCTGTAT830110.923201996521213No Hit
TGAGGTAGTAGATTGTATAGTT738700.8215408979896883No Hit
AGCAGCATTGTACAGGGCTATGA690600.768046763438038No Hit
TATTGCACTTGTCCCGGCCTGT681960.7584378378137915No Hit
TGTAAACATCCCCGACTGGAAGCT594840.6615478377693057No Hit
CCCATAAAGTAGAAAGCACTAC530850.5903817323647299No Hit
TTCAAGTAATTCAGGATAGGTT523100.5817626150513143No Hit
ACTGGACTTGGAGTCAGAAGGC512320.5697737009043956No Hit
TATTGCACTTGTCCCGGCCTGTA509950.5671379192227446No Hit
CAACGGAATCCCAAAAGCAGCTGT471750.524654011948877No Hit
CGCCTGTCTGAGCGTCGCT456500.507693813364414No Hit
CCCCCACTGCTAAATTTGACTGGCTT409570.4555008874910472No Hit
TGTCAGTTTGTCAAATACCCCAA360770.40122825202076595No Hit
CCCATAAAGTAGAAAGCACT340900.3791299473733379No Hit
TGTAAACATCCTACACTCTCAGCT319390.35520772628797415No Hit
CATTGCACTTGTCTCGGTCTGA315650.35104830709414525No Hit
TGAGGTAGTAGTTTGTACAGTT315290.3506479351931349No Hit
AACATTCAACGCTGTCGGTGAGTT275950.306896183566068No Hit
TTCTCACTACTGCACTTGACTAG274830.30565058209625834No Hit
TAGCTTATCAGACTGATGTTGACA258200.2871556245579227No Hit
GCCTGTCTGAGCGTCGCT251500.27970425862245374No Hit
CAACACCAGTCGATGGGCTGT240320.26727048680774584No Hit
CAAAGAATTCTCCTTTTGGGCT231310.25725006784079435No Hit
TCGTACGACTCTTAGCGG229420.25514811536049037No Hit
CACCCGGCTGTGTGCACATGTGC223250.24828618583484213No Hit
TGTCAGTTTGTCAAATACCCCA218480.24298125814645605No Hit
CTACGCCTGTCTGAGCGTCGCT213480.23742053729909116No Hit
CAACGGAATCCCAAAAGCAGCT212410.23623054303775506No Hit
CCCCCCACTGCTAAATTTGACTGGCT207470.23073655084055855No Hit
TTCACAGTGGCTAAGTTCCG202270.2249534011592991No Hit
TGAGGTAGTAGTTTGTGCTGTT200120.22256229119493215No Hit
TACGCCTGTCTGAGCGTCGCT199980.222406591011206No Hit
TAGCAGCACGTAAATATTGGC191400.2128643940371278No Hit
CTTCTCACTACTGCACTTGACTAG185720.20654741515452132No Hit
AACGGAATCCCAAAAGCAGCTG180640.20089772277359857No Hit
AACCGGGCGGAAACACCA178140.19811736234991612No Hit
TTCAAGTAATCCAGGATAGGCTT170710.1898541311707319No Hit
TGTAAACATCCCCGACTGGAAGC168530.18742965688128083No Hit
ACTGGACTTGGAGTCAGAAGGCA160110.17806540297431836No Hit
CAAAGAATTCTCCTTTTGGGCTT157890.17559644291808835No Hit
CCCCCCACTGCTAAATTTGACTGG150340.16719975443856738No Hit
ACTGGACTTGGAGTCAGAAGGCT143820.15994857445360358No Hit
TCGTTTCCCGGCCAATGCACCA143100.15914783065158303No Hit
TTCAAGTAATCCAGGATAGGC141470.15733503565534207No Hit
AGCAGCATTGTACAGGGCTATGT140960.15676784212891084No Hit
ACCCCCCACTGCTAAATTTGACTG139530.1551774759665645No Hit
TTATAAAGCAATGAGACTGATT138680.15423215342251245No Hit
CACTAGATTGTGAGCTCCTGGA138380.15389851017167056No Hit
CACTAGATTGTGAGCTCCTGGAA135870.1511070283062934No Hit
TGAGGTAGTAGGTTGTGTGGTT134030.14906068303446313No Hit
TGTAAACATCCTACACTCAGCT133980.14900507582598946No Hit
TATTGCACTTGTCCCGGCCTGTT133140.14807087472363217No Hit
CTCCCACTGCTTCACTTGACTAG129250.1437446339043823No Hit
TAGCAGCACGTAAATATTGGCGT128730.14316631893625636No Hit
ACCACAGGGTAGAACCACGGAC124510.13847307054108038No Hit
TTCACAGTGGCTAAGTTCCGC124130.13805045575668065No Hit
ACTGGACTTGGAGTCAGAAGG123830.13771681250583875No Hit
CCCCCCACTGCTAAATTTGACTGTA119670.13309029276083117No Hit
GTCAGGATGGCCGAGCGGTCTAAGG118920.13225618463372646No Hit
GGAATACCGGGTGCTGTAGGCTTT118050.13128861920628496No Hit
TGAGGTAGTAGGTTGTATAGT113730.1264841563941617No Hit
TTCACAGTGGCTAAGTTCTG112080.12464911851453127No Hit
CCCATAAAGTAGAAAGCACTA111330.12381501038742654No Hit
ACGCCTGTCTGAGCGTCGCT110740.12315884532743748No Hit
TTCACAGTGGCTAAGTTCTGC109430.1217019364654279No Hit
CAAAGTGCTGTTCGTGCAGGTAG108610.12078997824646004No Hit
AGCAGCATTGTACAGGGCTATG107900.12000035588613422No Hit
TATTGCACTTGTCCCGGCCTGTAG107790.1198780200274922No Hit
TCAGTGCACTACAGAACTTTGT107580.11964446975190288No Hit
ATCACATTGCCAGGGATTTCC107250.1192774621759768No Hit
ACCGGGTGCTGTAGGCTT105420.11724223834584124No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC104900.11666392337771529No Hit
CGCGACCTCAGATCAGACGTGGCG101970.11340534096115948No Hit
AGAGGTAGTAGGTTGCATAGTT100870.1121819823747392No Hit
CCCCCACTGCTAAATTTGACTGGCT100000.11121441694729771No Hit
CCCCCACTGCTAAATTTGACTGGCTA99250.11038030882019297No Hit
GCGTTCGCGCTTTCCCCTG97930.10891227851648866No Hit
CTCCCACTGCTTCACTTGACTAGCCT95530.10624313250975351No Hit
GGAATACCGGGTGCTGTAGGCTT94740.10536453861586985No Hit
AGCTCGGTCTGAGGCCCCTCAGT94130.10468613067249133No Hit
CAAAGTGCTGTTCGTGCAGGT93830.10435248742164945No Hit
ATCACATTGCCAGGGATTTCCA93410.10388538687047079No Hit
TAGCAGCACATCATGGTTTACA90940.10113839077187253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)