FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11830735
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG169347414.314190961085679No Hit
CAACGGAATCCCAAAAGCAGCTG10518628.890926895074566No Hit
CCCCCACTGCTAAATTTGACTG4923244.161398256321353No Hit
AAGCTGCCAGTTGAAGAACTGT4419223.7353723162593027No Hit
TAGCAGCACGTAAATATTGGCG3831023.238192724289742No Hit
TTCAAGTAATCCAGGATAGGCT3648683.0840687412912215No Hit
AACATTCAACGCTGTCGGTGAGT3634553.0721252737044655No Hit
AACATTCAACGCTGTCGGTGAG2083971.7614881915620626No Hit
TAGCTTATCAGACTGATGTTGAC1769131.4953677856870262No Hit
TAGCTTATCAGACTGATGTTGACT1198391.0129463638565144No Hit
TATTGCACTTGTCCCGGCCTGTAA1193511.0088215144705717No Hit
TATTGCACTTGTCCCGGCCTGTAT1149550.9716640597562196No Hit
TAGCTTATCAGACTGATGTTGA1062850.8983803626740012No Hit
TGTAAACATCCTTGACTGGAAGCT1056060.8926410742865933No Hit
TGAGGTAGTAGGTTGTATAGTT989740.8365836949268157No Hit
TATTGCACTTGTCCCGGCCTGT989270.8361864245966121No Hit
CATAAAGTAGAAAGCACTACT968970.8190277273559081No Hit
GCCTGTCTGAGCGTCGCT949560.8026213079745257No Hit
AGCAGCATTGTACAGGGCTATGA801100.6771345989915251No Hit
CGCCTGTCTGAGCGTCGCT717640.6065895314196456No Hit
TTCTCACTACTGCACTTGACTAG660510.5583000548993786No Hit
TGAGGTAGTAGATTGTATAGTT647800.5475568508634502No Hit
TATTGCACTTGTCCCGGCCTGTA639100.540203123474577No Hit
ACTGGACTTGGAGTCAGAAGGC638430.5396368019400316No Hit
CAACGGAATCCCAAAAGCAGCTGT628510.5312518622046728No Hit
TTCAAGTAATTCAGGATAGGTT615900.5205931837709153No Hit
CCCATAAAGTAGAAAGCACTAC528010.4463036320228625No Hit
TGTAAACATCCCCGACTGGAAGCT516190.4363127058462556No Hit
CTTCTCACTACTGCACTTGACTAG507420.4288998105358628No Hit
AACCGGGCGGAAACACCA462390.3908379318782814No Hit
TGTAAACATCCTACACTCTCAGCT462170.39065197555350534No Hit
CCCATAAAGTAGAAAGCACT397430.3359301007080287No Hit
CATTGCACTTGTCTCGGTCTGA394370.33334361728159745No Hit
CACCCGGCTGTGTGCACATGTGC392980.3321687114114212No Hit
CTCCCACTGCTTCACTTGACTAG372620.3149592988094146No Hit
CCCCCCACTGCTAAATTTGACTGG357210.30193390351487037No Hit
CTACGCCTGTCTGAGCGTCGCT351290.29692998786634983No Hit
TGTCAGTTTGTCAAATACCCCAA328940.2780385157811412No Hit
TGAGGTAGTAGTTTGTACAGTT310450.26240973193973155No Hit
CCCCCCACTGCTAAATTTGACTGGCT304830.2576593930977239No Hit
CCCCCACTGCTAAATTTGACTGGCTT302180.2554194646401935No Hit
TCGTACGACTCTTAGCGG298080.25195391495118435No Hit
TACGCCTGTCTGAGCGTCGCT288520.243873267383641No Hit
AACATTCAACGCTGTCGGTGAGTT276480.23369638488225797No Hit
GACCCGCGGGCGCTCTCCAGTCCT265690.22457607240801186No Hit
TGTCAGTTTGTCAAATACCCCA260400.2201046680531683No Hit
AACGGAATCCCAAAAGCAGCTG250730.2119310423232369No Hit
ACCCCCCACTGCTAAATTTGACTG247410.20912479233116116No Hit
TGTAAACATCCTACACTCAGCT234540.1982463473317592No Hit
TAGCAGCACGTAAATATTGGC234330.1980688435672002No Hit
ACGCCTGTCTGAGCGTCGCT232360.19640368920443235No Hit
AGCTCGGTCTGAGGCCCCTCAGT216550.1830401915012043No Hit
CAACGGAATCCCAAAAGCAGCT216470.1829725710194675No Hit
CAACACCAGTCGATGGGCTGT207170.17511169001756866No Hit
GCGTTCGCGCTTTCCCCTG194260.1641994347772983No Hit
TTCAAGTAATCCAGGATAGGCTT192260.16250892273387918No Hit
TGAGGTAGTAGTTTGTGCTGTT189700.1603450673183027No Hit
TAGCAGCACGTAAATATTGGCGT187780.15872217575662034No Hit
GGAATACCGGGTGCTGTAGGCTTT180780.15280538360465346No Hit
TAGCTTATCAGACTGATGTTGACA179780.15196012758294392No Hit
TGTAAACATCCCCGACTGGAAGC176300.14901863662739467No Hit
TTCAAGTAATCCAGGATAGGC173950.1470322849763772No Hit
TATTGCACTTGTCCCGGCCTGTT171020.1445556848327682No Hit
CAAAGAATTCTCCTTTTGGGCT169390.14317791751738165No Hit
AGCAGCATTGTACAGGGCTATGT167770.14180860276221213No Hit
TGATGTAGTAGGTTGTATAGTT164490.1390361630110048No Hit
TTATAAAGCAATGAGACTGATT159760.13503810202831862No Hit
CTCCCACTGCTTCACTTGACTAGCCT153630.12985668261523903No Hit
GGGCTACGCCTGTCTGAGCGTCGCT150580.1272786517490249No Hit
CAAAGAATTCTCCTTTTGGGCTT150200.12695745446077528No Hit
TTCACAGTGGCTAAGTTCCGC149770.12659399437144014No Hit
GGAATACCGGGTGCTGTAGGCTT146970.12422727751065339No Hit
ACCACAGGGTAGAACCACGGAC145050.12260438594897105No Hit
TTCACAGTGGCTAAGTTCCG142670.1205926766173023No Hit
TGAGGTAGTAGGTTGTGTGGTT142640.12056731893665101No Hit
ACTGGACTTGGAGTCAGAAGGCA141690.11976432571602694No Hit
TATTGCACTTGTCCCGGCCTGTAG140930.11912193113952768No Hit
AGCAGCATTGTACAGGGCTATG139500.11791321502848302No Hit
TTCACAGTGGCTAAGTTCTGC133300.11267262769388377No Hit
ATCCCACCGCTGCCACCA130630.11041579411591926No Hit
ACTGGACTTGGAGTCAGAAGGCT127810.1080321721346983No Hit
CACTAGATTGTGAGCTCCTGGA127730.10796455165296155No Hit
ATCACATTGCCAGGGATTTCC126350.10679809834300237No Hit
ACTGGACTTGGAGTCAGAAGG126280.10673893042148269No Hit
TGTCAGTTTGTCAAATACCCC126250.1067135727408314No Hit
CAAAGTGCTGTTCGTGCAGGT124280.10504841837806358No Hit
TAGCAGCACATCATGGTTTACA120910.10219990558490237No Hit
AGAGGTAGTAGGTTGCATAGTT119160.10072070754691065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)