FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9942010
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG142916614.375020745302006No Hit
CAACGGAATCCCAAAAGCAGCTG7914467.960623656584534No Hit
CCCCCACTGCTAAATTTGACTG4166884.191184679959083No Hit
TTCAAGTAATCCAGGATAGGCT2991173.008616969807916No Hit
TAGCAGCACGTAAATATTGGCG2953842.971069230467481No Hit
AACATTCAACGCTGTCGGTGAGT2855362.87201481390584No Hit
AAGCTGCCAGTTGAAGAACTGT2687882.703557932450279No Hit
TAGCTTATCAGACTGATGTTGAC1700161.7100767349861847No Hit
AACATTCAACGCTGTCGGTGAG1611371.6207688384944292No Hit
TAGCTTATCAGACTGATGTTGACT1514191.5230220046047027No Hit
TATTGCACTTGTCCCGGCCTGTAA1145331.1520105089413508No Hit
TGTAAACATCCTTGACTGGAAGCT1070791.0770357301994264No Hit
TATTGCACTTGTCCCGGCCTGTAT1055081.0612340965257527No Hit
GGAATACCGGGTGCTGTAGGCTTT1050441.0565670322198428No Hit
TAGCTTATCAGACTGATGTTGA937040.9425055899159225No Hit
TGAGGTAGTAGGTTGTATAGTT844750.8496772785382433No Hit
GGAATACCGGGTGCTGTAGGCTT714120.7182853366673339No Hit
CATAAAGTAGAAAGCACTACT672190.6761107663339707No Hit
AGCAGCATTGTACAGGGCTATGA620750.6243707258391412No Hit
TATTGCACTTGTCCCGGCCTGT605810.6093435834403707No Hit
ACTGGACTTGGAGTCAGAAGGC595780.5992550802101386No Hit
CCCATAAAGTAGAAAGCACTAC589260.5926970501940755No Hit
TGAGGTAGTAGATTGTATAGTT538200.5413392261725747No Hit
TGTAAACATCCCCGACTGGAAGCT537560.540695493164863No Hit
TATTGCACTTGTCCCGGCCTGTA503930.5068693352752612No Hit
CAACGGAATCCCAAAAGCAGCTGT491420.49428636664014625No Hit
TGAATACCGGGTGCTGTAGGCTTT464180.46688748049941614No Hit
TTCAAGTAATTCAGGATAGGTT440800.44337110906144733No Hit
TTCTCACTACTGCACTTGACTAG413140.41554977313440644No Hit
CCCCCACTGCTAAATTTGACTGGCTT410350.41274349955391315No Hit
GCCTGTCTGAGCGTCGCT409080.4114660918667352No Hit
TGTCAGTTTGTCAAATACCCCAA389720.39199316838345566No Hit
TGTAAACATCCTACACTCTCAGCT365840.3679738805332121No Hit
CTTCTCACTACTGCACTTGACTAG348580.35061320598148665No Hit
TGAATACCGGGTGCTGTAGGCTT322430.3243106776195156No Hit
CCCCCCACTGCTAAATTTTACTG296420.29814896585298145No Hit
CCCATAAAGTAGAAAGCACT288430.29011236158483045No Hit
CCCCCCACTGCTAAATTTGACTGGCT283380.2850329058208551No Hit
CACCCGGCTGTGTGCACATGTGC283280.2849323225384002No Hit
CATTGCACTTGTCTCGGTCTGA278290.27991321674389785No Hit
CCCCCCACTGCTAAATTTGACTGG260470.26198927581042464No Hit
TGTCAGTTTGTCAAATACCCCA248110.24955718209899203No Hit
TTAATGCTAATCGTGATAGGGGTT239400.24079637819716537No Hit
CAAAGAATTCTCCTTTTGGGCT238560.23995147862454375No Hit
TGAGGTAGTAGTTTGTACAGTT238540.23993136196805276No Hit
AACATTCAACGCTGTCGGTGAGTT227300.22862580102011565No Hit
TAGCTTATCAGACTGATGTTGACA214720.21597242408728218No Hit
CGCCTGTCTGAGCGTCGCT210920.21215025935399381No Hit
CAACGGAATCCCAAAAGCAGCT208720.20993742713998476No Hit
ACCCCCCACTGCTAAATTTGACTG196880.19802836649731795No Hit
AACCGGGCGGAAACACCA188400.18949890414513765No Hit
CAAAGAATTCTCCTTTTGGGCTT187080.18817120481673225No Hit
CTCCCACTGCTTCACTTGACTAG185050.18612936418289663No Hit
AACGGAATCCCAAAAGCAGCTG182020.18308169072451144No Hit
GGAATACCGGGTGCTGTAGGCTTA181880.1829408741290745No Hit
CTCCCACTGCTTCACTTGACTAGCCT181760.18282017419012855No Hit
TCCTGTCTGAGCGTCGCT180870.18192498297627946No Hit
TAGCAGCACGTAAATATTGGC180850.18190486631978847No Hit
ACTGGACTTGGAGTCAGAAGGCA179330.18037600042647312No Hit
TTCAAGTAATCCAGGATAGGCTT167060.16803443166924997No Hit
CTACGCCTGTCTGAGCGTCGCT166610.16758180689820268No Hit
TGTAAACATCCCCGACTGGAAGC160560.16149651830967782No Hit
CACTAGATTGTGAGCTCCTGGA152520.15340962240029934No Hit
CCCACCACTGCTAAATTTGACTG145330.14617768439178797No Hit
CCACCCACTGCTAAATTTGACTG144300.14514167658250193No Hit
CACTAGATTGTGAGCTCCTGGAA144050.14489021837636454No Hit
ACTGGACTTGGAGTCAGAAGGCT143500.14433701032286228No Hit
TCGTACGACTCTTAGCGG141860.142687444490601No Hit
TATTGCACTTGTCCCGGCCTGTAG140650.14147038677289603No Hit
TGAGGTAGTAGTTTGTGCTGTT140570.14138992014693205No Hit
GACCCGCGGGCGCTCTCCAGTCCT138540.13934807951309644No Hit
CACCCCACTGCTAAATTTGACTG137380.13818131343661896No Hit
CAACACCAGTCGATGGGCTGT131590.132357541382477No Hit
TGTAAACATCCTACACTCAGCT128560.12930986792409183No Hit
TATTGCACTTGTCCCGGCCTGTT127720.12846496835147017No Hit
TAGCAGCACGTAAATATTGGCGT127660.1284046183819972No Hit
TACGCCTGTCTGAGCGTCGCT126390.12712721069481928No Hit
AGCAGCATTGTACAGGGCTATGT123890.12461262863344535No Hit
TTATAAAGCAATGAGACTGATT122990.12370737909135074No Hit
TTCACAGTGGCTAAGTTCCGC122470.12318434602258496No Hit
CGCCCCACTGCTAAATTTGACTG120330.12103186377804889No Hit
ATCACATTGCCAGGGATTTCC114430.11509745011320648No Hit
TTCAAGTAATCCAGGATAGGC112710.11336741765498123No Hit
GGAATACCGGGTGCTGTAGGCT111580.11223082656324024No Hit
ATCACATTGCCAGGGATTTCCA109250.10988723608203975No Hit
CCCCACACTGCTAAATTTGACTG108640.10927367805906453No Hit
CCCCCAACTGCTAAATTTGACTG106360.10698037921909151No Hit
ACTGGACTTGGAGTCAGAAGG103370.10397293907368832No Hit
TGTCAGTTTGTCAAATACCCC103000.10360078092860499No Hit
GCCCCCACTGCTAAATTTGACTG101930.10252453980633695No Hit
ACCCCCACTGCTAAATTTGACTG100640.10122701546266802No Hit
TTCACAGTGGCTAAGTTCTGC99680.10026141595110043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)