FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11095401
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG176610215.91742380469169No Hit
CAACGGAATCCCAAAAGCAGCTG114033810.277573564037928No Hit
CCCCCACTGCTAAATTTGACTG6303955.681588254448848No Hit
TTCAAGTAATCCAGGATAGGCT4055663.6552622117938776No Hit
TAGCAGCACGTAAATATTGGCG2305032.077464347615737No Hit
AACATTCAACGCTGTCGGTGAGT2300922.0737601101573526No Hit
AAGCTGCCAGTTGAAGAACTGT1901521.713791146439863No Hit
CATAAAGTAGAAAGCACTACT1869751.6851576612688446No Hit
AACATTCAACGCTGTCGGTGAG1361051.2266794142906596No Hit
TAGCTTATCAGACTGATGTTGA947120.8536149346923108No Hit
TAGCTTATCAGACTGATGTTGAC873750.7874884377770573No Hit
CCCATAAAGTAGAAAGCACTAC755610.6810118895207122No Hit
TTCAAGTAATTCAGGATAGGTT666330.6005461181619303No Hit
CATTGCACTTGTCTCGGTCTGA662240.596859906189961No Hit
TCGTACGACTCTTAGCGG655550.5908303809839771No Hit
ACTGGACTTGGAGTCAGAAGGC654320.589721813569424No Hit
CAACGGAATCCCAAAAGCAGCTGT649560.5854317478025355No Hit
CCCATAAAGTAGAAAGCACT641410.5780863620882202No Hit
CACCCGGCTGTGTGCACATGTGC610370.5501108071713677No Hit
AGCAGCATTGTACAGGGCTATGA561980.5064981427890709No Hit
TAGCTTATCAGACTGATGTTGACT533760.4810641814568035No Hit
TATTGCACTTGTCCCGGCCTGTAA470660.4241937718159082No Hit
TATTGCACTTGTCCCGGCCTGT458520.4132523015617011No Hit
AACCGGGCGGAAACACCA449610.40522194736359685No Hit
TATTGCACTTGTCCCGGCCTGTAT442740.39903019277987334No Hit
CAACACCAGTCGATGGGCTGT441540.3979486635949435No Hit
CAACGGAATCCCAAAAGCAGCT420440.3789317754265934No Hit
TTCTCACTACTGCACTTGACTAG414170.3732807854353349No Hit
CTTCTCACTACTGCACTTGACTAG391800.35311927887960065No Hit
CTCCCACTGCTTCACTTGACTAG381220.34358379656580235No Hit
CGCCTGTCTGAGCGTCGCT342540.3087225058382297No Hit
CACTAGATTGTGAGCTCCTGGA329580.2970419906409872No Hit
TGTAAACATCCTACACTCTCAGCT322900.29102147817821095No Hit
TGAGGTAGTAGGTTGTATAGTT304220.27418567386613607No Hit
CTACGCCTGTCTGAGCGTCGCT298820.2693187925339517No Hit
TGAGGTAGTAGATTGTATAGTT298710.26921965235866646No Hit
CCCCCCACTGCTAAATTTGACTGG297320.2679668810527894No Hit
ATCCCGGACGAGCCCCCA295570.2663896509914333No Hit
TATTGCACTTGTCCCGGCCTGTA268430.24192906592560287No Hit
CCCCCACTGCTAAATTTGACTGGCTT264900.2387475675732675No Hit
CACTAGATTGTGAGCTCCTGGAA264760.23862138916835907No Hit
CAAAGAATTCTCCTTTTGGGCT260530.23480899879148126No Hit
GCCTGTCTGAGCGTCGCT255410.23019447426911385No Hit
TGTAAACATCCTTGACTGGAAGCT245570.22132593495268896No Hit
CCCCCCACTGCTAAATTTGACTGGCT245280.22106456539966424No Hit
TTCAAGTAATCCAGGATAGGCTT241870.21799121996582188No Hit
AGCTCGGTCTGAGGCCCCTCAGT229290.2066531890104738No Hit
CAAAGAATTCTCCTTTTGGGCTT222520.2005515618588278No Hit
AACGGAATCCCAAAAGCAGCTG222200.20026315407617987No Hit
CCCATAAAGTAGAAAGCACTA205390.185112732743954No Hit
TTCACAGTGGCTAAGTTCCG200630.18082266697706553No Hit
TCAGTGCACTACAGAACTTTGT199910.18017374946610762No Hit
TTCACAGTGGCTAAGTTCCGC196520.17711842951868076No Hit
GACCCGCGGGCGCTCTCCAGTCCT173530.15639813288406612No Hit
TACGCCTGTCTGAGCGTCGCT172410.15538870564479826No Hit
ACTGGACTTGGAGTCAGAAGGCT171590.15464966070176284No Hit
ACTGGACTTGGAGTCAGAAGG167630.15108061439149428No Hit
TGTAAACATCCTACACTCAGCT166270.14985488131524044No Hit
ACCCCCCACTGCTAAATTTGACTG165990.14960252450542347No Hit
CCCCCCACTGCTAAATTTGACTGT165790.14942226964126848No Hit
TTCAAGTAATCCAGGATAGGC164720.14845790611803936No Hit
ACCGGGTGCTGTAGGCTTA164640.14838580417237737No Hit
AACATTCAACGCTGTCGGTGAGTT163070.1469708034887608No Hit
TGTCAGTTTGTCAAATACCCCA150250.13541646669642673No Hit
TGTAAACATCCCCGACTGGAAGCT148860.13416369539054965No Hit
ACCACAGGGTAGAACCACGGAC147910.1333074847858135No Hit
TGTCAGTTTGTCAAATACCCCAA143920.1297114002459217No Hit
ACTGGACTTGGAGTCAGAAGGCA141100.12716980666133654No Hit
TCGTTTCCCGGCCAATGCACCA140700.1268092969330266No Hit
CCCCCCACTGCTAAATTTGACTGA138720.12502477377789228No Hit
ACTTGACTTGGAGTCAGAAGGC137220.12367286229672998No Hit
TAGCAGCACGTAAATATTGGC135180.1218342626823492No Hit
CAGTGCAATAGTATTGTCAAAGC130490.11760728611791497No Hit
TCGTTTCCCGGGCGGCGCACCA129250.11648970596015412No Hit
AGCAGCATTGTACAGGGCTATGT127380.11480432298030507No Hit
ACGCCTGTCTGAGCGTCGCT126600.11410132901010067No Hit
TCCTGTCTGAGCGTCGCT124870.11254212443516012No Hit
CCCACCACTGCTAAATTTGACTG122040.10999151810736718No Hit
ATCACATTGCCAGGGATTTCC120710.10879282326073658No Hit
CAAAGTGCTGTTCGTGCAGGT117170.1056023121651935No Hit
ATCCCACCGCTGCCACCA115920.10447571926422489No Hit
ACCGGGTGCTGTAGGCTTT113350.10215944425983343No Hit
ACCGGGTGCTGTAGGCTT113330.10214141877341794No Hit
CCACCCACTGCTAAATTTGACTG113250.10206931682775593No Hit
GGAATACCGGGTGCTGTAGGCTTT112950.10179893453152347No Hit
CATAAAGTAGAAAGCACTAC112530.10142039931679801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)