FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6104587
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4546657.447923995513538No Hit
CAACGGAATCCCAAAAGCAGCTG3406895.580868943304437No Hit
AAGCTGCCAGTTGAAGAACTGT2945284.824699852750071No Hit
TAGCAGCACGTAAATATTGGCG2521454.130418650762124No Hit
TTCAAGTAATCCAGGATAGGCT2271573.7210871103974767No Hit
AACATTCAACGCTGTCGGTGAGT1954983.202477088130614No Hit
CCCCCACTGCTAAATTTGACTG1603642.6269426580373088No Hit
TAGCTTATCAGACTGATGTTGAC1212911.9868829783243322No Hit
CATAAAGTAGAAAGCACTACT1078821.7672284791747583No Hit
TAGCTTATCAGACTGATGTTGA983171.6105430228121904No Hit
AACATTCAACGCTGTCGGTGAG955681.5655113114122217No Hit
TAGCTTATCAGACTGATGTTGACT738461.2096805238421535No Hit
TGAGGTAGTAGGTTGTATAGTT659571.080449832232713No Hit
TGTAAACATCCTTGACTGGAAGCT634241.0389564437364887No Hit
TATTGCACTTGTCCCGGCCTGT606890.9941540680802812No Hit
CGCCTGTCTGAGCGTCGCT567740.930021965449915No Hit
TGAGGTAGTAGATTGTATAGTT533040.8731794632462441No Hit
TTCAAGTAATTCAGGATAGGTT501920.8222014036330386No Hit
TATTGCACTTGTCCCGGCCTGTAT485130.7946974955062479No Hit
TATTGCACTTGTCCCGGCCTGTAA473480.7756134854004046No Hit
TTCTCACTACTGCACTTGACTAG405800.664746034416415No Hit
AGCAGCATTGTACAGGGCTATGA389700.6383724238838762No Hit
TATTGCACTTGTCCCGGCCTGTA329920.5404460612978405No Hit
TGTCAGTTTGTCAAATACCCCAA326670.5351221958176696No Hit
TGTAAACATCCTACACTCTCAGCT288680.4728903036356104No Hit
ACTGGACTTGGAGTCAGAAGGC283750.46481440923030504No Hit
CATTGCACTTGTCTCGGTCTGA283650.4646505979847613No Hit
GCCTGTCTGAGCGTCGCT272430.446270976234756No Hit
TGTAAACATCCCCGACTGGAAGCT250310.41003592872048505No Hit
CCCATAAAGTAGAAAGCACT250000.40952811385929955No Hit
TGAGGTAGTAGTTTGTACAGTT248450.4069890395533719No Hit
CTTCTCACTACTGCACTTGACTAG247060.4047120632403142No Hit
TGTCAGTTTGTCAAATACCCCA237500.3890517081663346No Hit
CCCATAAAGTAGAAAGCACTAC230650.37783063784658977No Hit
TTAATGCTAATCGTGATAGGGGTT227070.3719661952561246No Hit
TTCACAGTGGCTAAGTTCCG222240.36405411209636296No Hit
AACCGGGCGGAAACACCA206870.33887632365629317No Hit
TGTAAACATCCTACACTCAGCT192850.31590998703106365No Hit
CAACGGAATCCCAAAAGCAGCTGT190530.3121095661344494No Hit
TTATAAAGCAATGAGACTGATT168090.27535032263443865No Hit
TACGCCTGTCTGAGCGTCGCT166710.2730897274459353No Hit
CTACGCCTGTCTGAGCGTCGCT158640.2598701599305571No Hit
GCGTTCGCGCTTTCCCCTG156010.2555619241727573No Hit
ACGCCTGTCTGAGCGTCGCT152120.2491896667211066No Hit
AACATTCAACGCTGTCGGTGAGTT150170.24599534743300405No Hit
TGAGGTAGTAGTTTGTGCTGTT144900.23736249479285004No Hit
CAACACCAGTCGATGGGCTGT144860.23729697029463254No Hit
CACCCGGCTGTGTGCACATGTGC140220.22969612850140395No Hit
CTCCCACTGCTTCACTTGACTAG132400.21688608909988505No Hit
ATCCCGGACGAGCCCCCA121760.19945657257403326No Hit
TAGCAGCACGTAAATATTGGCGT118920.19480433320059162No Hit
TAGCAGCACGTAAATATTGGC115330.1889235094855721No Hit
TTCAAGTAATCCAGGATAGGC114920.18825188337884283No Hit
CCCCCCACTGCTAAATTTGACTGG114110.18692501228993869No Hit
TGTCAGTTTGTCAAATACC113800.18641719742875318No Hit
ACCGGGTGCTGTAGGCTT113670.18620424280954634No Hit
TTCAAGTAATCCAGGATAGGCTT113100.18527051870994712No Hit
TGTCAGTTTGTCAAATACCCC105780.17327953553614683No Hit
TAGCAGCACATAATGGTTTGT105020.17203457007001455No Hit
AACGGAATCCCAAAAGCAGCTG102600.16807033792785653No Hit
TGATGTAGTAGGTTGTATAGTT98820.16187827284630393No Hit
ACTGGACTTGGAGTCAGAAGG98100.16069883187838915No Hit
TGTAAACATCCCCGACTGGAAGC96970.15884776480374513No Hit
TCCTGTCTGAGCGTCGCT96860.15866757243364701No Hit
ATCCCACCGCTGCCACCA95640.15666907523801366No Hit
TAGCTTATCAGACTGATGTTGACA94550.1548835326615871No Hit
TCGTACGACTCTTAGCGG94090.15413000093208598No Hit
TATTGCACTTGTCCCGGCCTGTT93230.15272122422040998No Hit
CAAAGAATTCTCCTTTTGGGCT92340.1512633041350709No Hit
AACATTCAACGCTGTCGGTGA88150.14439961294678902No Hit
AGCAGCATTGTACAGGGCTATG87700.1436624623418423No Hit
TTCACAGTGGCTAAGTTCCGC86530.14174587076898076No Hit
CAAAGTGCTGTTCGTGCAGGT86040.14094319566581656No Hit
AATGACACGATCACTCCCGTTG84590.1385679326054326No Hit
TTCACAGTGGCTAAGTTCTGC81440.13340787837080542No Hit
CAAAGAATTCTCCTTTTGGGCTT80370.13165509804348763No Hit
TGTCAGTTTGTCAAATACCC79790.13070499281933406No Hit
TGATGTAGTAGATTGTATAGTT79740.13062308719656218No Hit
ACCGGGTGCTGTAGGCTTT79570.1303446080791379No Hit
CCCCCCACTGCTAAATTTGACTGGCT78820.12911602373755995No Hit
TGAGAACTGAATTCCATGGGTT78340.1283297297589501No Hit
CATAAAGTAGAAAGCACTAC77500.12695371529638289No Hit
TGAGGTAGTAGGTTGTATAGT74970.12280929078412675No Hit
GACCCGCGGGCGCTCTCCAGTCCT74750.12244890604393058No Hit
TTCACAGTGGCTAAGTTCTG74120.12141689519700515No Hit
CCCCCACTGCTAAATTTGACTGGCTT72210.11828810040712008No Hit
CCCATAAAGTAGAAAGCACTA71310.11681379919722662No Hit
AGAGGTAGTAGGTTGCATAGTT71020.11633874658514984No Hit
AGCAGCATTGTACAGGGCTATGT70870.11609302971683423No Hit
ACCGGGTGCTGTAGGCTTA70360.11525759236456128No Hit
TGAGGTAGTAGGTTGTGTGGTT69330.11357033653546095No Hit
CAACGGAATCCCAAAAGCAGCT68750.11262023131130738No Hit
CACTAGATTGTGAGCTCCTGGA68020.11142440921883823No Hit
ACCACAGGGTAGAACCACGGAC67640.11080192648577208No Hit
ACTGGACTTGGAGTCAGAAGGCA67390.1103923983719128No Hit
AGCTCGGTCTGAGGCCCCTCAGT65210.10682131321905969No Hit
TGTAAACATCCTTGACTGGAAGC64240.10523234413728562No Hit
TAGTGGGTTATCAGAACT64110.10501938951807878No Hit
TAGCAGCACATCATGGTTTACA63940.10474091040065445No Hit
CAAAGTGCTGTTCGTGCAGGTAG61610.10092410837948579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)