FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7949124
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG153230519.276400770701272No Hit
CAACGGAATCCCAAAAGCAGCTG5317706.689667943285323No Hit
CCCCCACTGCTAAATTTGACTG3947084.96542763705787No Hit
AACATTCAACGCTGTCGGTGAGT2410393.0322712288800626No Hit
TAGCAGCACGTAAATATTGGCG1964382.47119053621506No Hit
AAGCTGCCAGTTGAAGAACTGT1737582.1858760789239167No Hit
TTCAAGTAATCCAGGATAGGCT1703152.1425631302266765No Hit
AACATTCAACGCTGTCGGTGAG1414341.7792400772714076No Hit
TAGCTTATCAGACTGATGTTGAC1354371.7037978021225986No Hit
TATTGCACTTGTCCCGGCCTGTAA1012671.2739391158069744No Hit
TAGCTTATCAGACTGATGTTGACT960981.208913082749747No Hit
TATTGCACTTGTCCCGGCCTGTAT891191.1211172451203428No Hit
TGTAAACATCCTTGACTGGAAGCT758520.9542183516070449No Hit
AGCAGCATTGTACAGGGCTATGA666860.8389100484531377No Hit
ACTGGACTTGGAGTCAGAAGGC650480.8183040043154441No Hit
TGAGGTAGTAGGTTGTATAGTT587310.7388361283582946No Hit
CGCCTGTCTGAGCGTCGCT573580.721563784890008No Hit
TAGCTTATCAGACTGATGTTGA516610.6498955104990185No Hit
TATTGCACTTGTCCCGGCCTGT485250.6104446225772802No Hit
TATTGCACTTGTCCCGGCCTGTA461370.5804035765450382No Hit
TGTAAACATCCCCGACTGGAAGCT455230.5726794549940345No Hit
TGAGGTAGTAGATTGTATAGTT370360.46591297355532507No Hit
CCCCCACTGCTAAATTTGACTGGCTT321980.405050921334225No Hit
CATAAAGTAGAAAGCACTACT298380.3753621153727128No Hit
CAACGGAATCCCAAAAGCAGCTGT280070.35232813074748864No Hit
CTTCTCACTACTGCACTTGACTAG273430.3439750090701818No Hit
TTCAAGTAATTCAGGATAGGTT262020.3296212261879422No Hit
TTCTCACTACTGCACTTGACTAG244180.3071785016814431No Hit
CCCCCCACTGCTAAATTTGACTGG243350.30613436147177975No Hit
TGTCAGTTTGTCAAATACCCCAA240340.30234778071143437No Hit
TACGCCTGTCTGAGCGTCGCT238340.2998317802062215No Hit
AACATTCAACGCTGTCGGTGAGTT234540.2950513792463169No Hit
TGTAAACATCCTACACTCTCAGCT229040.2881323778569815No Hit
CCCCCCACTGCTAAATTTGACTGGCT228160.2870253376346878No Hit
GCCTGTCTGAGCGTCGCT225840.28410677704864085No Hit
CCCATAAAGTAGAAAGCACTAC221150.2782067558639166No Hit
ACCCCCCACTGCTAAATTTGACTG207700.26128665246635985No Hit
CACCCGGCTGTGTGCACATGTGC201140.2530341708092615No Hit
CATTGCACTTGTCTCGGTCTGA196050.24663094952349468No Hit
ACTGGACTTGGAGTCAGAAGGCA182730.22987438615877676No Hit
CTACGCCTGTCTGAGCGTCGCT181000.22769804572176758No Hit
TAGCTTATCAGACTGATGTTGACA166070.2089161019503533No Hit
GCGTTCGCGCTTTCCCCTG162590.2045382610712828No Hit
AACGGAATCCCAAAAGCAGCTG156050.19631093941923664No Hit
TGAGGTAGTAGTTTGTACAGTT154540.1944113590378009No Hit
CTCCCACTGCTTCACTTGACTAG153490.19309045877256412No Hit
CAACGGAATCCCAAAAGCAGCT153020.1924991986538391No Hit
CAAAGAATTCTCCTTTTGGGCT147510.18556761726197754No Hit
TGTCAGTTTGTCAAATACCCCA146160.18386931692095884No Hit
CCCATAAAGTAGAAAGCACT144090.18126525639806348No Hit
GGAATACCGGGTGCTGTAGGCTTT139160.1750633151527137No Hit
ACTGGACTTGGAGTCAGAAGGCT136790.1720818545540364No Hit
ACTGGACTTGGAGTCAGAAGG129280.16263427265696195No Hit
TTCAAGTAATCCAGGATAGGCTT123730.15565237125499615No Hit
AGCAGCATTGTACAGGGCTATGT123140.15491015110595832No Hit
TAGCAGCACGTAAATATTGGC122950.15467113105796312No Hit
CAAAGAATTCTCCTTTTGGGCTT122510.15411761094681628No Hit
ACGCCTGTCTGAGCGTCGCT122420.1540043909240817No Hit
AACCGGGCGGAAACACCA121740.1531489507523093No Hit
TATTGCACTTGTCCCGGCCTGTAG121510.15285961069420984No Hit
TGTAAACATCCCCGACTGGAAGC119840.15075875027235705No Hit
CAACACCAGTCGATGGGCTGT115860.1457519092669834No Hit
CACTAGATTGTGAGCTCCTGGAA114460.14399070891333435No Hit
GACCCGCGGGCGCTCTCCAGTCCT112130.14105956832476133No Hit
CACTAGATTGTGAGCTCCTGGA105050.13215292653630764No Hit
TGAGGTAGTAGTTTGTGCTGTT104730.13175036645547358No Hit
CTCCCACTGCTTCACTTGACTAGCCT103080.12967466603867295No Hit
TCCTGTCTGAGCGTCGCT102580.1290456659123697No Hit
TATTGCACTTGTCCCGGCCTGTT102000.128316025765858No Hit
GGAATACCGGGTGCTGTAGGCTT100970.12702028550567332No Hit
TGTAAACATCCTACACTCAGCT100910.12694480549051695No Hit
TAGCAGCACGTAAATATTGGCGT100610.12656740541473502No Hit
TTATAAAGCAATGAGACTGATT99060.124617505023195No Hit
TCGTACGACTCTTAGCGG97860.12310790472006727No Hit
TCGTTTCCCGGCCAATGCACCA97260.1223531045685034No Hit
ATCACATTGCCAGGGATTTCCA94020.1182771837500585No Hit
CCAAGTCCTTCTGATCGAGGCCC92400.11623922334083606No Hit
AGCAGCATTGTACAGGGCTATG91540.1151573431235945No Hit
GCCCCCACTGCTAAATTTGACTG91000.11447802298718703No Hit
TTCACAGTGGCTAAGTTCCG86370.10865348181761914No Hit
ACCACAGGGTAGAACCACGGAC84580.10640166136545359No Hit
GGGCTACGCCTGTCTGAGCGTCGCT82780.10413726091076199No Hit
GTCAGGATGGCCGAGCGGTCTAAGG79900.1005142201832554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)