FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7111624
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6626049.31719674718461No Hit
CCCCCCACTGCTAAATTTGACTG5345297.516271951385506No Hit
AAGCTGCCAGTTGAAGAACTGT3169264.4564504535110405No Hit
TAGCAGCACGTAAATATTGGCG2498123.512727894500609No Hit
AACATTCAACGCTGTCGGTGAGT2311483.250284323243186No Hit
TTCAAGTAATCCAGGATAGGCT2308013.2454049876652644No Hit
CCCCCACTGCTAAATTTGACTG2141923.011857769758356No Hit
AACATTCAACGCTGTCGGTGAG1309401.8412109526600395No Hit
TAGCTTATCAGACTGATGTTGAC1277581.7964673047956417No Hit
TAGCTTATCAGACTGATGTTGACT989641.391580882228869No Hit
TGTAAACATCCTTGACTGGAAGCT894741.2581373818413348No Hit
TGAGGTAGTAGGTTGTATAGTT806421.1339463391202909No Hit
TATTGCACTTGTCCCGGCCTGTAA700600.98514769622241No Hit
TATTGCACTTGTCCCGGCCTGTAT629890.8857189300221722No Hit
TATTGCACTTGTCCCGGCCTGT617680.8685498558416475No Hit
TAGCTTATCAGACTGATGTTGA599720.8432954273173048No Hit
AGCAGCATTGTACAGGGCTATGA592090.8325665136402036No Hit
CATAAAGTAGAAAGCACTACT562580.7910710689991484No Hit
GCCTGTCTGAGCGTCGCT506050.7115814896850565No Hit
TGAGGTAGTAGATTGTATAGTT456760.6422724261012674No Hit
CAACGGAATCCCAAAAGCAGCTGT439710.6182975927861203No Hit
ACTGGACTTGGAGTCAGAAGGC434320.6107184519316544No Hit
TGTAAACATCCCCGACTGGAAGCT426480.5996942470524314No Hit
TTCAAGTAATTCAGGATAGGTT385750.5424218153265696No Hit
CCCATAAAGTAGAAAGCACTAC381560.5365300527699439No Hit
TATTGCACTTGTCCCGGCCTGTA346580.4873429753879001No Hit
TGTCAGTTTGTCAAATACCCCAA335740.47210032476407643No Hit
TGTAAACATCCTACACTCTCAGCT302680.425613052658577No Hit
CGCCTGTCTGAGCGTCGCT302630.42554274522950036No Hit
TTCTCACTACTGCACTTGACTAG276580.3889125746805512No Hit
CATTGCACTTGTCTCGGTCTGA241390.3394302060963853No Hit
TGTCAGTTTGTCAAATACCCCA229100.32214864002933785No Hit
TGAGGTAGTAGTTTGTACAGTT222410.3127415060188784No Hit
CCCATAAAGTAGAAAGCACT218810.3076793711253576No Hit
CCCCCACTGCTAAATTTGACTGGCTT210490.2959802149269984No Hit
AACATTCAACGCTGTCGGTGAGTT199050.2798938751542545No Hit
AACCGGGCGGAAACACCA182420.25650962424335144No Hit
CTTCTCACTACTGCACTTGACTAG175960.24742590440664466No Hit
CACCCGGCTGTGTGCACATGTGC172870.243080905289706No Hit
CAAAGAATTCTCCTTTTGGGCT166180.2336737712792465No Hit
TAGCAGCACGTAAATATTGGC158780.22326827177589817No Hit
CAACACCAGTCGATGGGCTGT157560.22155277050642722No Hit
CTCCCACTGCTTCACTTGACTAG156210.21965446992135693No Hit
TAGCTTATCAGACTGATGTTGACA155610.21881078077243676No Hit
AACGGAATCCCAAAAGCAGCTG152010.21374864587891598No Hit
TGAGGTAGTAGTTTGTGCTGTT146000.20529769290389932No Hit
CTACGCCTGTCTGAGCGTCGCT140830.1980279047373708No Hit
TCGTACGACTCTTAGCGG140110.19701547775866665No Hit
CCCCCCACTGCTAAATTTGACTGG138890.19529997648919573No Hit
CAACGGAATCCCAAAAGCAGCT136450.19186897395025382No Hit
TGTAAACATCCTACACTCAGCT135730.1908565469715497No Hit
TACGCCTGTCTGAGCGTCGCT134960.18977381256376882No Hit
CCCCCCACTGCTAAATTTGACTGGCT134440.1890426153013714No Hit
TTCAAGTAATCCAGGATAGGCTT131520.1849366614432934No Hit
CAAAGAATTCTCCTTTTGGGCTT128220.18029637112423266No Hit
TGTAAACATCCCCGACTGGAAGC124790.17547328148957259No Hit
TTCACAGTGGCTAAGTTCCG124050.17443273153923772No Hit
TAGCAGCACGTAAATATTGGCGT115630.16259296048272517No Hit
ACTGGACTTGGAGTCAGAAGGCA113600.15973847886221207No Hit
TATTGCACTTGTCCCGGCCTGTT111020.15611061552185548No Hit
TTCACAGTGGCTAAGTTCCGC110780.15577313986228744No Hit
GGAATACCGGGTGCTGTAGGCTTT108460.15251087515312958No Hit
AGCTCGGTCTGAGGCCCCTCAGT104100.14638006733764328No Hit
AGCAGCATTGTACAGGGCTATGT103960.14618320653622857No Hit
TTCAAGTAATCCAGGATAGGC102600.14427084446534294No Hit
TTATAAAGCAATGAGACTGATT102450.14405992217811292No Hit
CTCCCACTGCTTCACTTGACTAGCCT102110.14358183166039148No Hit
ACTGGACTTGGAGTCAGAAGGCT100940.1419366378199972No Hit
ATCACATTGCCAGGGATTTCC100230.1409382723271084No Hit
ACCACAGGGTAGAACCACGGAC99570.14001021426329627No Hit
TTAATGCTAATCGTGATAGGGGTT98730.1388290494548081No Hit
ATCACATTGCCAGGGATTTCCA97740.13743696235908986No Hit
TTCACAGTGGCTAAGTTCTGC97690.13736665493001318No Hit
TGAGAACTGAATTCCATGGGTT97020.13642453538038568No Hit
ACTGGACTTGGAGTCAGAAGG96360.13549647731657355No Hit
CAAAGTGCTGTTCGTGCAGGTAG96210.1352855550293435No Hit
ACCCCCCACTGCTAAATTTGACTG95180.13383722199036394No Hit
ACGCCTGTCTGAGCGTCGCT92610.1302234201358227No Hit
TGATGTAGTAGGTTGTATAGTT91870.12918287018548788No Hit
TGAGGTAGTAGGTTGTGTGGTT89460.125794052103992No Hit
AGCAGCATTGTACAGGGCTATG87340.12281301711114084No Hit
TAGCAGCACATCATGGTTTACA86120.12109751584166992No Hit
CACTAGATTGTGAGCTCCTGGA83400.11727279169989864No Hit
TCGTTTCCCGGCCAATGCACCA83400.11727279169989864No Hit
GGAATACCGGGTGCTGTAGGCTT82930.11661190186657787No Hit
AGAGGTAGTAGGTTGCATAGTT80340.11296997704040596No Hit
TATTGCACTTGTCCCGGCCTGTAG79130.11126853725675036No Hit
CCCCCCACTGCTAAATTTGACTGTA78130.10986238867521679No Hit
TGTCAGTTTGTCAAATACCCC77690.10924368329934203No Hit
GGGCTACGCCTGTCTGAGCGTCGCT77660.10920149884189603No Hit
TGAGAACTGAATTCCATAGGCTGT76570.10766879688802444No Hit
CACTAGATTGTGAGCTCCTGGAA75240.10579861927458482No Hit
CAAAGTGCTGTTCGTGCAGGT73740.1036893964022845No Hit
GCGTTCGCGCTTTCCCCTG71240.10017402494845058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)