FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14173542
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG12642198.919569998804814No Hit
CAACGGAATCCCAAAAGCAGCTG9972717.036145234550403No Hit
CCCCCACTGCTAAATTTGACTG5417793.822467242133265No Hit
AACATTCAACGCTGTCGGTGAGT4709883.32300846182274No Hit
AAGCTGCCAGTTGAAGAACTGT4385103.0938631994740624No Hit
TAGCAGCACGTAAATATTGGCG4126702.9115516784724664No Hit
TTCAAGTAATCCAGGATAGGCT3476752.452985993197748No Hit
AACATTCAACGCTGTCGGTGAG2775351.9581202779093612No Hit
TAGCTTATCAGACTGATGTTGAC2343581.6534892971707424No Hit
CGCCTGTCTGAGCGTCGCT2223191.5685493435585827No Hit
TAGCTTATCAGACTGATGTTGACT1568021.1063007397868507No Hit
GCCTGTCTGAGCGTCGCT1507921.0638977892752568No Hit
TATTGCACTTGTCCCGGCCTGTAA1505951.062507875589602No Hit
TGAGGTAGTAGGTTGTATAGTT1427041.0068337187698035No Hit
ACTGGACTTGGAGTCAGAAGGC1348810.9516393291105356No Hit
TATTGCACTTGTCCCGGCCTGT1345670.9494239336927918No Hit
TATTGCACTTGTCCCGGCCTGTAT1333370.9407457924067251No Hit
AGCAGCATTGTACAGGGCTATGA1329410.9379518542365769No Hit
TGTAAACATCCTTGACTGGAAGCT1248110.8805914569555021No Hit
TAGCTTATCAGACTGATGTTGA983520.6939126437131946No Hit
TATTGCACTTGTCCCGGCCTGTA901020.6357055985017718No Hit
CATAAAGTAGAAAGCACTACT821790.5798056689005473No Hit
TGAGGTAGTAGATTGTATAGTT764910.5396745570020536No Hit
TACGCCTGTCTGAGCGTCGCT678330.4785889088274477No Hit
TGTAAACATCCCCGACTGGAAGCT675320.47646523360215814No Hit
TTAATGCTAATCGTGATAGGGGTT652630.460456532319162No Hit
TCCTGTCTGAGCGTCGCT627860.442980307956896No Hit
TTCAAGTAATTCAGGATAGGTT620800.43799919596668213No Hit
CAACGGAATCCCAAAAGCAGCTGT604160.42625901133252364No Hit
GCGTTCGCGCTTTCCCCTG555140.3916734433778091No Hit
CATTGCACTTGTCTCGGTCTGA546880.38584568345724735No Hit
CCCATAAAGTAGAAAGCACTAC466670.3292543247129052No Hit
AACATTCAACGCTGTCGGTGAGTT464000.327370533067881No Hit
TGTCAGTTTGTCAAATACCCCAA431800.30465214693687714No Hit
CTACGCCTGTCTGAGCGTCGCT413460.29171254440139244No Hit
TGTAAACATCCTACACTCTCAGCT402460.2839516050398694No Hit
CACCCGGCTGTGTGCACATGTGC383710.27072273112818235No Hit
ACGCCTGTCTGAGCGTCGCT346940.2447800274624367No Hit
CCCATAAAGTAGAAAGCACT343140.24209897568300145No Hit
TGAGGTAGTAGTTTGTACAGTT342920.241943756895771No Hit
CAACGGAATCCCAAAAGCAGCT339870.23979186007280326No Hit
ACTGGACTTGGAGTCAGAAGGCA335470.23668748432819406No Hit
TTCTCACTACTGCACTTGACTAG314880.22216041692330685No Hit
ACTGGACTTGGAGTCAGAAGGCT304140.2145829179466925No Hit
AACCGGGCGGAAACACCA303560.21417370478035766No Hit
TTATAAAGCAATGAGACTGATT299090.2110199412398115No Hit
AACGGAATCCCAAAAGCAGCTG294670.20790145469636312No Hit
CAACACCAGTCGATGGGCTGT283590.20008407213948357No Hit
ACTGGACTTGGAGTCAGAAGG281160.19836961008052892No Hit
CCCCCACTGCTAAATTTGACTGGCTT276200.19487013196842398No Hit
CCTCGACACAAGGGTTTGT273760.19314861451004978No Hit
TAGCAGCACGTAAATATTGGC270850.19109549327895597No Hit
TGAGGTAGTAGTTTGTGCTGTT268890.18971263499272095No Hit
TCGTTCGCGCTTTCCCCTG267290.18858377108559032No Hit
ACCACAGGGTAGAACCACGGAC265890.18759601516685104No Hit
AGCAGCATTGTACAGGGCTATGT261260.18432936523559176No Hit
TGTCAGTTTGTCAAATACCCCA260650.18389898587099823No Hit
CCCCCCACTGCTAAATTTGACTGG254260.1793905856418953No Hit
CTCCCACTGCTTCACTTGACTAG243230.17160848008211357No Hit
AGCAGCATTGTACAGGGCTATG229670.16204135846918152No Hit
TAGCTTATCAGACTGATGTTGACA228990.161561591308651No Hit
TATTGCACTTGTCCCGGCCTGTT228500.16121587673709226No Hit
TTCAAGTAATCCAGGATAGGCTT226710.15995296024098987No Hit
CACTAGATTGTGAGCTCCTGGA226480.15979068605433983No Hit
CTTCTCACTACTGCACTTGACTAG226290.1596566334653681No Hit
ACCGGGTGCTGTAGGCTT222200.15677097510276541No Hit
TGTAAACATCCTACACTCAGCT218740.15432980690359546No Hit
TTCACAGTGGCTAAGTTCCG208410.14704157930318335No Hit
TGAGGTAGTAGGTTGTGTGGTT207470.14637837175774412No Hit
TAGCAGCACGTAAATATTGGCGT197430.13929475074049943No Hit
ATAGGCGATAGAAATTGAAACCT194040.13690297033726642No Hit
CAAAGTGCTGTTCGTGCAGGTAG193610.13659958816222506No Hit
CTGCGTTCGCGCTTTCCCCTG193060.13621154119414894No Hit
CCCCCCACTGCTAAATTTGACTGGCT186640.1316819747667873No Hit
GGAATACCGGGTGCTGTAGGCTTT181510.12806255486454973No Hit
CACTAGATTGTGAGCTCCTGGAA180860.1276039539022779No Hit
CAAGTCCTTCTGATCGAGGCCC177060.1249229021228427No Hit
CCAAGTCCTTCTGATCGAGGCCC173050.12209368695559655No Hit
TGTAAACATCCCCGACTGGAAGC171000.12064733007458545No Hit
CTCGACACAAGGGTTTGT170820.12052033288503325No Hit
CCCCCCACTGCTAAATTTGACTGTA169270.11942674597500047No Hit
TACCTGGTTGATCCTGCC166650.11757823132707407No Hit
ATCCCGGACGAGCCCCCA160260.11306983109797114No Hit
CAAAGAATTCTCCTTTTGGGCTT157820.11134831363959694No Hit
CCCCCCACTGCTAAATTTGACTGT154270.10884364684565086No Hit
TTCACAGTGGCTAAGTTCCGC151590.10695279980120706No Hit
CAAAGAATTCTCCTTTTGGGCT144610.10202813100634973No Hit
CTCCCACTGCTTCACTTGACTAGCCT142420.10048299853346468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)