FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12753601
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG11026318.645644473274647No Hit
CCCCCCACTGCTAAATTTGACTG8716166.834273708264826No Hit
AACATTCAACGCTGTCGGTGAGT5386264.223324847625388No Hit
AAGCTGCCAGTTGAAGAACTGT5095893.995647974246646No Hit
TAGCAGCACGTAAATATTGGCG4122163.2321538050312224No Hit
TTCAAGTAATCCAGGATAGGCT4078473.1978968136136614No Hit
CCCCCACTGCTAAATTTGACTG3721672.918132690524033No Hit
AACATTCAACGCTGTCGGTGAG2759342.1635771732234685No Hit
TATTGCACTTGTCCCGGCCTGTAA1643271.288475309836022No Hit
TAGCTTATCAGACTGATGTTGAC1623841.2732403969670998No Hit
TGAGGTAGTAGGTTGTATAGTT1551111.2162133659348446No Hit
TATTGCACTTGTCCCGGCCTGTAT1486821.1658040736886783No Hit
TAGCTTATCAGACTGATGTTGACT1485571.1648239583471367No Hit
TATTGCACTTGTCCCGGCCTGT1447851.135247997800778No Hit
TGTAAACATCCTTGACTGGAAGCT1346771.0559919508223599No Hit
AGCAGCATTGTACAGGGCTATGA1001490.7852605707203792No Hit
CATAAAGTAGAAAGCACTACT983580.7712174781067715No Hit
ACTGGACTTGGAGTCAGAAGGC974260.7639097381202375No Hit
TAGCTTATCAGACTGATGTTGA932800.7314012724719866No Hit
TGTAAACATCCCCGACTGGAAGCT828490.649612607451025No Hit
CGCCTGTCTGAGCGTCGCT825900.6475818084633509No Hit
TTAATGCTAATCGTGATAGGGGTT820530.6433712329560882No Hit
TGAGGTAGTAGATTGTATAGTT817840.6412620247410907No Hit
TATTGCACTTGTCCCGGCCTGTA793440.6221301732741992No Hit
CCCATAAAGTAGAAAGCACTAC778260.6102276525665182No Hit
CAACGGAATCCCAAAAGCAGCTGT768220.6023553661432564No Hit
GCCTGTCTGAGCGTCGCT765310.6000736576281476No Hit
TTCAAGTAATTCAGGATAGGTT575230.45103339833196915No Hit
CCCATAAAGTAGAAAGCACT488190.382786006869746No Hit
TGTAAACATCCTACACTCTCAGCT473860.37154996459431344No Hit
CATTGCACTTGTCTCGGTCTGA446250.34990117693034306No Hit
AACATTCAACGCTGTCGGTGAGTT441410.34610617032789404No Hit
TGTCAGTTTGTCAAATACCCCAA426680.3345564911431681No Hit
TCGTACGACTCTTAGCGG422610.33136523559110875No Hit
TGAGGTAGTAGTTTGTACAGTT407080.3191882825877962No Hit
CACCCGGCTGTGTGCACATGTGC380680.29848824657443807No Hit
GGAATACCGGGTGCTGTAGGCTTT363610.2851037914703463No Hit
AACCGGGCGGAAACACCA350700.27498116022290486No Hit
TACGCCTGTCTGAGCGTCGCT341530.2677910340773559No Hit
CTACGCCTGTCTGAGCGTCGCT339620.2662934178354804No Hit
TTCTCACTACTGCACTTGACTAG319470.25049395852983014No Hit
AACGGAATCCCAAAAGCAGCTG293040.22977039974827504No Hit
CCCCCACTGCTAAATTTGACTGGCTT287490.2254186876318304No Hit
GGAATACCGGGTGCTGTAGGCTT276110.2164957175624359No Hit
CAACGGAATCCCAAAAGCAGCT272300.21350832600141714No Hit
TGTCAGTTTGTCAAATACCCCA267910.21006616092192318No Hit
ACTGGACTTGGAGTCAGAAGGCA257580.20196648773942355No Hit
TATTGCACTTGTCCCGGCCTGTT257400.20182535113024155No Hit
CAAAGAATTCTCCTTTTGGGCT254610.1996377336879208No Hit
TGAGGTAGTAGTTTGTGCTGTT253840.19903398263753116No Hit
CAACACCAGTCGATGGGCTGT248200.19461170221649557No Hit
TGTAAACATCCCCGACTGGAAGC243930.19126362820978954No Hit
ACTGGACTTGGAGTCAGAAGGCT241120.18906032892200408No Hit
TGAGAACTGAATTCCATGGGTT239740.18797828158494218No Hit
CTCCCACTGCTTCACTTGACTAG237310.18607293736098535No Hit
TTCAAGTAATCCAGGATAGGCTT225030.1764442842456809No Hit
TAGCAGCACGTAAATATTGGC221840.17394302989406676No Hit
TCCTGTCTGAGCGTCGCT215680.1691130214909499No Hit
CTTCTCACTACTGCACTTGACTAG215470.16894836211357092No Hit
ACGCCTGTCTGAGCGTCGCT211340.16571006102511754No Hit
ACCACAGGGTAGAACCACGGAC210730.16523176473844525No Hit
TGAGGTAGTAGGTTGTGTGGTT203530.15958630037116575No Hit
TAGCTTATCAGACTGATGTTGACA201630.15809652505202257No Hit
CACTAGATTGTGAGCTCCTGGA198390.1555560660867468No Hit
AGCTCGGTCTGAGGCCCCTCAGT195620.15338413048989066No Hit
CAAAGAATTCTCCTTTTGGGCTT192990.15132196781128718No Hit
ACTGGACTTGGAGTCAGAAGG192940.1512827631976255No Hit
CCCCCCACTGCTAAATTTGACTGG191970.15052219369258926No Hit
TATTGCACTTGTCCCGGCCTGTAG187520.14703298307670123No Hit
AGCAGCATTGTACAGGGCTATGT187360.14690752831298393No Hit
TTCACAGTGGCTAAGTTCCG181530.142336270360034No Hit
CCCCCCACTGCTAAATTTGACTGGCT181490.14230490666910467No Hit
TTCAAGTAATCCAGGATAGGC180200.14129342763663377No Hit
CACTAGATTGTGAGCTCCTGGAA174840.13709069305210347No Hit
TGAGAACTGAATTCCATAGGCTGT173870.13633012354706722No Hit
TGTAAACATCCTACACTCAGCT173330.13590671371952126No Hit
GCGTTCGCGCTTTCCCCTG164470.12895965617867455No Hit
ATCCCGGACGAGCCCCCA160870.12613692399503482No Hit
TTATAAAGCAATGAGACTGATT159200.12482748989873527No Hit
TAGCAGCACGTAAATATTGGCGT159150.1247882852850736No Hit
CGCGACCTCAGATCAGACGTGGCG157360.12338476011598608No Hit
ACCGGGTGCTGTAGGCTT155960.12228703093345951No Hit
TCGTTTCCCGGCCAATGCACCA152250.1193780485997641No Hit
CAAAGTGCTGTTCGTGCAGGTAG152090.11925259383604678No Hit
AGCAGCATTGTACAGGGCTATG150020.11762952283045393No Hit
AACATTCAACGCTGTCGGTGA146990.11525372324255713No Hit
ACCCCCCACTGCTAAATTTGACTG146010.11448531281478855No Hit
CTCCCACTGCTTCACTTGACTAGCCT142790.11196053569497745No Hit
CCCCCCACTGCTAAATTTGACTGTA142730.11191349015858346No Hit
TTCACAGTGGCTAAGTTCTGC142630.11183508093126013No Hit
TATTGCACTTGTCCCGGCCTGTTT135400.10616609379578365No Hit
CCCATAAAGTAGAAAGCACTA134770.10567211566364669No Hit
AGAGGTAGTAGGTTGCATAGTT134520.10547609259533837No Hit
TGAGGTAGTAGGTTGTATAGT131960.10346881637586121No Hit
CAAAGTGCTGTTCGTGCAGGT131870.10339824807127022No Hit
TTCACAGTGGCTAAGTTCCGC131330.10297483824372428No Hit
TACCACCCTGAACGCGCCCGA130980.10270040594809263No Hit
CCCCCCACTGCTAAATTTGACTGT130060.10197904105671801No Hit
ATCACATTGCCAGGGATTTCC129510.10154779030643972No Hit
AACATTCAACGCTGTCGGTGAGA127780.10019131067374619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)