FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6713460
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG115579717.216115088195952No Hit
CCCCCCACTGCTAAATTTGACTG4618856.879984389569612No Hit
TCGTACGACTCTTAGCGG2665793.970813857533969No Hit
CCCCCACTGCTAAATTTGACTG2344833.49272953141897No Hit
TTCAAGTAATCCAGGATAGGCT1886192.8095646656120685No Hit
TAGCAGCACGTAAATATTGGCG1622482.4167567841321764No Hit
TAGCTTATCAGACTGATGTTGAC1493762.2250225666049994No Hit
TGAGAACTGAATTCCATGGGTT1248181.8592201338802943No Hit
AAGCTGCCAGTTGAAGAACTGT1130471.683885805530978No Hit
TGAGGTAGTAGATTGTATAGTT991971.4775838390338216No Hit
AACATTCAACGCTGTCGGTGAG887241.3215838032847445No Hit
TTAATGCTAATCGTGATAGGGGTT822261.2247931766927933No Hit
TAGCTTATCAGACTGATGTTGA795641.1851414918685745No Hit
TATTGCACTTGTCCCGGCCTGT679261.0117882582155848No Hit
TAGCTTATCAGACTGATGTTGACT610710.9096799563861258No Hit
TGTAAACATCCTTGACTGGAAGCT527240.7853476448805833No Hit
CAACGGAATCCCAAAAGCAGCTGT517010.7701096007125983No Hit
CAACGGAATCCCAAAAGCAGCT494460.7365203635681155No Hit
CATAAAGTAGAAAGCACTACT485880.7237400684594828No Hit
AGCAGCATTGTACAGGGCTATGA451370.6723358744969061No Hit
TATTGCACTTGTCCCGGCCTGTAA436490.6501714466162009No Hit
TGAGAACTGAATTCCATAGGCTGT391880.5837228493206186No Hit
CATTGCACTTGTCTCGGTCTGA391430.5830525541226134No Hit
TTCAAGTAATTCAGGATAGGTT383980.5719554447334162No Hit
TATTGCACTTGTCCCGGCCTGTAT383460.571180881393499No Hit
TGAGGTAGTAGGTTGTATAGTT351580.5236941904770417No Hit
TATTGCACTTGTCCCGGCCTGTA346500.5161273024640052No Hit
TGAGGTAGTAGTTTGTACAGTT331720.4941118290717454No Hit
TCGTTTCCCGGCCAATGCACCA288910.4303444125681839No Hit
ACTGGACTTGGAGTCAGAAGGC286760.4271418910666035No Hit
AACGGAATCCCAAAAGCAGCTG274460.4088204889877946No Hit
CCCATAAAGTAGAAAGCACTAC262570.3911098003116128No Hit
CCCATAAAGTAGAAAGCACT243320.3624360612858347No Hit
TGTAAACATCCCCGACTGGAAGCT242800.36166149794591756No Hit
CCCCCACTGCTAAATTTGACTGGCTT237440.35367753736523344No Hit
TTCTCACTACTGCACTTGACTAG230190.3428783369529274No Hit
TAGCTTATCAGACTGATGTTGACA222900.33201955474524314No Hit
CACCCGGCTGTGTGCACATGTGC214160.31900093245509764No Hit
TGTCAGTTTGTCAAATACCCCA212450.3164538107026779No Hit
AACATTCAACGCTGTCGGTGAGT199340.29692587726745967No Hit
CAAAGAATTCTCCTTTTGGGCT181700.27065030550565583No Hit
TAGCAGCACGTAAATATTGGC178170.26539221206352615No Hit
TGAGAACTGAATTCCATGGGTTG177570.2644984851328525No Hit
CTCCCACTGCTTCACTTGACTAGCCT175650.261638558954697No Hit
CTCCCACTGCTTCACTTGACTAG173940.25909143720227723No Hit
CCCATAAAGTAGAAAGCACTA171950.2561272428822098No Hit
TGTAAACATCCTACACTCTCAGCT156780.2335308469850122No Hit
TTAATGCTAATCGTGATAGGGGTTT136480.20329308583055533No Hit
TTCACAGTGGCTAAGTTCCGC131630.1960687931409437No Hit
GCCTGTCTGAGCGTCGCT131590.19600921134556548No Hit
CTTCTCACTACTGCACTTGACTAG125970.18763796909492272No Hit
CACTAGATTGTGAGCTCCTGGA123180.18348213886729048No Hit
TTAATGCTAATCGTGATAGGGGT111060.16542885486768374No Hit
GGAATACCGGGTGCTGTAGGCTTT109640.16331370113175622No Hit
CACTAGATTGTGAGCTCCTGGAA107570.16023034322093227No Hit
TCGTTTCCCGGCCAACGCACCA106260.1582790394222949No Hit
GGAATACCGGGTGCTGTAGGCTT106170.15814498038269387No Hit
CAACACCAGTCGATGGGCTGT104510.15567233587449691No Hit
ACCCCCCACTGCTAAATTTGACTG103430.15406362739928442No Hit
AATGCACCCGGGCAAGGATTCT101760.15157608744224288No Hit
TACCACCCTGAACGCGCCCGA101100.15059298781850194No Hit
TGAGGTAGTAGATTGTATAGT100590.14983331992742938No Hit
TATTGCACTTGTCCCGGCCTGTT98270.14637757579549143No Hit
CAAAGTGCTGTTCGTGCAGGTAG94430.1406577234391804No Hit
ACTGGACTTGGAGTCAGAAGGCT93600.13942140118508192No Hit
CCCCCCACTGCTAAATTTGACTGG92810.13824466072636166No Hit
TGAGGTAGTAGTTTGTGCTGTT91220.13587628436007662No Hit
TGATAACTGAATTCCATGGGTT88710.13213752670009205No Hit
ACTGGACTTGGAGTCAGAAGGCA86810.12930739141962566No Hit
TTCACAGTGGCTAAGTTCTGC85810.12781784653516964No Hit
ATCACATTGCCAGGGATTTCC84660.12610486991804523No Hit
CTCGTACGACTCTTAGCGG84600.12601549722497787No Hit
AGCTACATTGTCTGCTGGGTTT83090.1237662844494493No Hit
TCAGTGCACTACAGAACTTTGT82050.12221715776961506No Hit
CAAAGAATTCTCCTTTTGGGCTT81650.12162133981583267No Hit
TTCAAGTAATCCAGGATAGGC80610.12007221313599843No Hit
ACCGGGTGCTGTAGGCTT80070.1192678588983922No Hit
CCCCCCACTGCTAAATTTGACTGGCT79480.11838902741656314No Hit
CAAAGAATTCTCCTTTTGGGC78980.11764425497433516No Hit
TGAGAACTGAATTCCATGGGTTT78880.11749530048588955No Hit
AATCCTTGGAACCTAGGTGTGAGT78620.11710801881593098No Hit
TTCAAGTAATCCAGGATAGGCTT77680.11570784662454234No Hit
AGCAGCATTGTACAGGGCTATG76810.11441194257506562No Hit
TGATGTAGTAGATTGTATAGTT75940.11311603852558891No Hit
AGCAGCATTGTACAGGGCTATGT75170.11196908896455778No Hit
TGTCAGTTTGTCAAATACCCCAA73020.10876656746297735No Hit
ACTGGACTTGGAGTCAGAAGG72630.10818564495803952No Hit
TCGATTCCCGGCCAACGCACCA72240.10760472245310167No Hit
TTCACAGTGGCTAAGTTCCG72090.10738129072043329No Hit
TGTAAACATCCCCGACTGGA71900.10709827719238664No Hit
TTATAAAGCAATGAGACTGATT71220.10608538667095654No Hit
CACCCGTAGAACCGACCTTGCG69530.1035680558162259No Hit
AGCTCGGTCTGAGGCCCCTCAGT69380.10334462408355752No Hit
TTCACAGTGGCTAAGTTCTG69190.10306161055551087No Hit
TCCCACTGCTTCACTTGACTAG68980.10274880612977512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)