FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9516950
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7916248.318043070521544No Hit
CCCCCCACTGCTAAATTTGACTG5842306.138836496986954No Hit
AACATTCAACGCTGTCGGTGAGT3096803.2539836817467784No Hit
TTCAAGTAATCCAGGATAGGCT2963973.11441165499451No Hit
CCCCCACTGCTAAATTTGACTG2864123.009493587756582No Hit
AAGCTGCCAGTTGAAGAACTGT2600972.7329869338390975No Hit
TAGCAGCACGTAAATATTGGCG2525012.6531714467345107No Hit
AACATTCAACGCTGTCGGTGAG1904332.0009877113991354No Hit
TCGTACGACTCTTAGCGG1879381.974771329049748No Hit
TATTGCACTTGTCCCGGCCTGT1371181.440776719432171No Hit
TATTGCACTTGTCCCGGCCTGTAT1129021.186325450906015No Hit
TATTGCACTTGTCCCGGCCTGTAA1078801.1335564440288117No Hit
CATAAAGTAGAAAGCACTACT945880.9938898491638603No Hit
TAGCTTATCAGACTGATGTTGAC774340.8136430263897573No Hit
ATCCCGGACGAGCCCCCA751600.7897488165851455No Hit
TAGCTTATCAGACTGATGTTGACT737640.7750802515511798No Hit
GCCTGTCTGAGCGTCGCT723490.7602120427237717No Hit
TGAGGTAGTAGGTTGTATAGTT722390.7590562102354221No Hit
TAGCTTATCAGACTGATGTTGA717310.7537183656528614No Hit
TATTGCACTTGTCCCGGCCTGTA664810.6985536332543514No Hit
CGCCTGTCTGAGCGTCGCT654950.6881931711315075No Hit
ACTGGACTTGGAGTCAGAAGGC483310.5078412726766454No Hit
AGCAGCATTGTACAGGGCTATGA471420.4953477742343923No Hit
CAACGGAATCCCAAAAGCAGCTGT465100.48870699121041933No Hit
CCCATAAAGTAGAAAGCACT458990.48228686711604035No Hit
CCCATAAAGTAGAAAGCACTAC456680.4798596188905059No Hit
TTCAAGTAATTCAGGATAGGTT448500.4712644282044143No Hit
CATTGCACTTGTCTCGGTCTGA441640.46405623650434225No Hit
TGAGGTAGTAGATTGTATAGTT415520.436610468690074No Hit
TGTAAACATCCTTGACTGGAAGCT412780.4337313950372756No Hit
CACCCGGCTGTGTGCACATGTGC366100.3846820672589432No Hit
CAACACCAGTCGATGGGCTGT362530.38093086545584454No Hit
CTACGCCTGTCTGAGCGTCGCT340240.3575094962146486No Hit
TCCTGTCTGAGCGTCGCT340120.3573834053977377No Hit
TGTAAACATCCCCGACTGGAAGCT314340.3302948948980503No Hit
ACCGGGTGCTGTAGGCTT311890.3277205407194532No Hit
CAACGGAATCCCAAAAGCAGCT293910.308827933318973No Hit
CTCCCACTGCTTCACTTGACTAG282170.2964920483978586No Hit
ACCGGGTGCTGTAGGCTTA275940.2899458334865687No Hit
TTCTCACTACTGCACTTGACTAG253720.26659801722190407No Hit
ACGCCTGTCTGAGCGTCGCT252950.26578893448005925No Hit
AACATTCAACGCTGTCGGTGAGTT240510.2527175197936314No Hit
TATTGCACTTGTCCCGGCCTGTT232690.24450060155827236No Hit
TACGCCTGTCTGAGCGTCGCT232280.2440697912671602No Hit
TTCACAGTGGCTAAGTTCCG224480.23587388816795296No Hit
CACTAGATTGTGAGCTCCTGGA221300.23253248151981465No Hit
AACCGGGCGGAAACACCA220730.23193355013948797No Hit
AGCTCGGTCTGAGGCCCCTCAGT207040.21754868944357172No Hit
ACCGGGTGCTGTAGGCTTT205270.21568884989413625No Hit
AACGGAATCCCAAAAGCAGCTG180160.18930434645553462No Hit
TGAGGTAGTAGTTTGTACAGTT171030.1797109368022318No Hit
ATCCCACCGCTGCCACCA169670.1782819075439085No Hit
CTTCTCACTACTGCACTTGACTAG165960.17438359978774712No Hit
TGTAAACATCCTACACTCTCAGCT159770.1678794151487609No Hit
TTCAAGTAATCCAGGATAGGC159440.16753266540225598No Hit
CACTAGATTGTGAGCTCCTGGAA157080.165052879336342No Hit
ACCACAGGGTAGAACCACGGAC153050.16081832940175161No Hit
CCCCCACTGCTAAATTTGACTGGCTT153040.1608078218336757No Hit
TTCACAGTGGCTAAGTTCTG149310.15688849894136253No Hit
TCGTTTCCCGGCCAATGCACCA142260.14948066344784833No Hit
TTCAAGTAATCCAGGATAGGCTT140130.1472425514476802No Hit
CCCATAAAGTAGAAAGCACTA138840.14588707516588825No Hit
ACTGGACTTGGAGTCAGAAGG137040.14399571291222504No Hit
TATTGCACTTGTCCCGGCCTGTAG131920.13861583805736083No Hit
TAGCAGCACGTAAATATTGGC131070.13772269477090876No Hit
TATTGCACTTGTCCCGGCCTGA130920.13756508124977015No Hit
ACTGGACTTGGAGTCAGAAGGCT128160.13466499246081992No Hit
TTAATGCTAATCGTGATAGGGGTT125420.13178591880802148No Hit
TGAGGTAGTAGTTTGTGCTGTT125200.13155475231035155No Hit
CAAAGTGCTGTTCGTGCAGGT124660.13098734363425257No Hit
ATACCGGGTGCTGTAGGCTTA122550.12877024677023627No Hit
ATACCGGGTGCTGTAGGCTT120420.12653213477006814No Hit
TATTGCACTTGTCCCGGCCTGTTT120120.12621690772779093No Hit
TGATGTAGTAGGTTGTATAGTT119770.1258491428451342No Hit
TCGTTTCCCGGGCGGCGCACCA118230.12423097736144458No Hit
TATTGCACTTGTCCCGGCCTG117960.12394727302339509No Hit
TACCACCCTGAACGCGCCCGA117340.12329580380268888No Hit
CAAAGAATTCTCCTTTTGGGCT116350.12225555456317413No Hit
CCCCCCACTGCTAAATTTGACTGG113510.11927140522961664No Hit
TTCACAGTGGCTAAGTTCTGC111330.11698075538906898No Hit
TTATAAAGCAATGAGACTGATT111010.11664451321063997No Hit
CTCGACACAAGGGTTTGT109230.11477416609312857No Hit
ATACCGGGTGCTGTAGGCTTT108670.1141857422808778No Hit
AATGACACGATCACTCCCGTTG108080.11356579576439932No Hit
TTCACCACCTTCTCCACCCAGC107920.1133976746751848No Hit
CCCCCCACTGCTAAATTTGACTGGCT107390.11284077356716175No Hit
ACTGGACTTGGAGTCAGAAGGCA103880.1091526171725185No Hit
AGCAGCATTGTACAGGGCTATG99820.1048865445337004No Hit
TGAGAACTGAATTCCATGGGTT99630.10468690074025817No Hit
ACTTGACTTGGAGTCAGAAGGC97630.10258538712507684No Hit
CCCCCCACTGCTAAATTTGACTGA96520.1014190470686512No Hit
TTCACAGTGGCTAAGTTCCGC96020.10089366866485586No Hit
GGAATACCGGGTGCTGTAGGCTTT95960.10083062325640041No Hit
TGTCAGTTTGTCAAATACCCCAA95850.10071504000756545No Hit
AGCAGCATTGTACAGGGCTATGT95300.10013712376339058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)