FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7662752
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6602628.61651270979408No Hit
CCCCCCACTGCTAAATTTGACTG4386455.724379439658232No Hit
TTCAAGTAATCCAGGATAGGCT2326353.035919732231971No Hit
AACATTCAACGCTGTCGGTGAGT1870372.4408593674961687No Hit
CCCCCACTGCTAAATTTGACTG1755342.2907435866383254No Hit
TAGCAGCACGTAAATATTGGCG1676122.187360363482989No Hit
AAGCTGCCAGTTGAAGAACTGT1330461.736269162828185No Hit
AACATTCAACGCTGTCGGTGAG1132561.478006857066495No Hit
TCGTACGACTCTTAGCGG967421.2624968157654066No Hit
CATAAAGTAGAAAGCACTACT760440.9923849812704366No Hit
TATTGCACTTGTCCCGGCCTGT643070.8392154672368362No Hit
TATTGCACTTGTCCCGGCCTGTAA598420.7809465842036908No Hit
CGCCTGTCTGAGCGTCGCT570070.7439494322666321No Hit
TAGCTTATCAGACTGATGTTGAC547460.7144430617094224No Hit
TATTGCACTTGTCCCGGCCTGTAT539660.704263951123565No Hit
TAGCTTATCAGACTGATGTTGA505490.6596716166724436No Hit
ACTGGACTTGGAGTCAGAAGGC504710.6586537056138578No Hit
TGAGGTAGTAGGTTGTATAGTT472570.6167105499434146No Hit
ATCCCGGACGAGCCCCCA462250.6032428036298186No Hit
CCCATAAAGTAGAAAGCACTAC434550.5670939109082481No Hit
CAACGGAATCCCAAAAGCAGCTGT424110.5534695628933313No Hit
AGCAGCATTGTACAGGGCTATGA407830.5322239320807981No Hit
TAGCTTATCAGACTGATGTTGACT399670.521575016390978No Hit
TTCAAGTAATTCAGGATAGGTT385950.5036702218732905No Hit
GCCTGTCTGAGCGTCGCT364320.47544276521020123No Hit
CACCCGGCTGTGTGCACATGTGC361420.47165822409494657No Hit
CATTGCACTTGTCTCGGTCTGA351380.4585558817510994No Hit
CCCATAAAGTAGAAAGCACT351170.45828182877378776No Hit
TGAGGTAGTAGATTGTATAGTT346480.45216131228049666No Hit
TATTGCACTTGTCCCGGCCTGTA323140.42170228137358484No Hit
CTCCCACTGCTTCACTTGACTAG294050.38373941894504743No Hit
CAACGGAATCCCAAAAGCAGCT275210.35915295183766877No Hit
TGTAAACATCCTTGACTGGAAGCT249080.3250529313750465No Hit
CAACACCAGTCGATGGGCTGT248770.3246483769799675No Hit
TTCTCACTACTGCACTTGACTAG244180.31865836190444374No Hit
ACCGGGTGCTGTAGGCTTA243900.318292957934695No Hit
CTACGCCTGTCTGAGCGTCGCT238630.3114155332183529No Hit
ACCGGGTGCTGTAGGCTT237440.3098625663469208No Hit
TACGCCTGTCTGAGCGTCGCT198610.2591888658278384No Hit
CTTCTCACTACTGCACTTGACTAG191420.24980581389036208No Hit
ACCGGGTGCTGTAGGCTTT186510.24339819427798265No Hit
CACTAGATTGTGAGCTCCTGGA183990.2401095585502441No Hit
CCCCCACTGCTAAATTTGACTGGCTT178230.2325926768868417No Hit
TGTAAACATCCCCGACTGGAAGCT169260.22088669971310568No Hit
AGCTCGGTCTGAGGCCCCTCAGT164130.21419197698163794No Hit
CCCCCCACTGCTAAATTTGACTGGCT162550.21213005458091297No Hit
TGTAAACATCCTACACTCTCAGCT161930.21132094579075508No Hit
ACGCCTGTCTGAGCGTCGCT161800.21115129394765744No Hit
TTCACAGTGGCTAAGTTCCG161490.2107467395525785No Hit
CCCCCCACTGCTAAATTTGACTGG158670.20706659957153775No Hit
AACGGAATCCCAAAAGCAGCTG158390.20670119560178904No Hit
CGCGACCTCAGATCAGACGTGGCG155040.20232939810658104No Hit
CACTAGATTGTGAGCTCCTGGAA151710.19798370089492653No Hit
AACATTCAACGCTGTCGGTGAGTT147130.19200673596117948No Hit
CAAAGAATTCTCCTTTTGGGCT142600.18609502173631615No Hit
TTAATGCTAATCGTGATAGGGGTT142150.18550776535636282No Hit
AACCGGGCGGAAACACCA140850.1838112469253866No Hit
TACCACCCTGAACGCGCCCGA139380.1818928760842058No Hit
ATCCCACCGCTGCCACCA136920.17868254120712768No Hit
ACTGGACTTGGAGTCAGAAGGCT129470.16896018558345618No Hit
TTCAAGTAATCCAGGATAGGCTT122430.15977288577263105No Hit
ACTGGACTTGGAGTCAGAAGG120840.15769791323012933No Hit
TCGTTTCCCGGGCGGCGCACCA119950.15653645061199944No Hit
TCGTTTCCCGGCCAATGCACCA117510.15335221601847485No Hit
GGAATACCGGGTGCTGTAGGCTTT116150.15157739673683815No Hit
TCCTGTCTGAGCGTCGCT112570.14690544598076513No Hit
ACTGGACTTGGAGTCAGAAGGCA111870.14599193605639332No Hit
ACCACAGGGTAGAACCACGGAC111780.14587448478040266No Hit
CCCATAAAGTAGAAAGCACTA108610.141737589837176No Hit
CAAAGAATTCTCCTTTTGGGCTT106890.13949296545157666No Hit
TTCAAGTAATCCAGGATAGGC101680.13269384158589498No Hit
CCCCCCACTGCTAAATTTGACTGT100350.13095817272958854No Hit
CGCGACCTCAGATCAGACGTGGC100250.13082767131182113No Hit
CGCGACCTCAGATCAGACGT100100.13063191918517003No Hit
TGAGGTAGTAGTTTGTACAGTT99900.1303709163496352No Hit
TAGCAGCACGTAAATATTGGC99310.12960095798480756No Hit
TTCACAGTGGCTAAGTTCCGC99100.129326905007496No Hit
TATTGCACTTGTCCCGGCCTGTT97800.12763038657651976No Hit
CAAAGTGCTGTTCGTGCAGGT93740.12233202901516323No Hit
ACCCCCCACTGCTAAATTTGACTG92970.12132716809835423No Hit
GGAATACCGGGTGCTGTAGGCTT92290.12043975845753589No Hit
TACCACCCTGAACGCGCCCGAT91220.11904339328742466No Hit
TGTCAGTTTGTCAAATACCCCAA90480.1180776827959459No Hit
AGCAGCATTGTACAGGGCTATGT85350.11138296006447813No Hit
CTCCCACTGCTTCACTTGACTAGCCT84720.1105608011325435No Hit
TGAGGTAGTAGTTTGTGCTGTT83560.10904698468644164No Hit
CGCGACCTCAGATCAGACGTG83260.10865548043313943No Hit
TTCACAGTGGCTAAGTTCTGC83170.10853802915714876No Hit
CAAAGTGCTGTTCGTGCAGGTAG82130.10718081441236776No Hit
CGCGACCTCAGATCAGACGTGG81810.10676320987551208No Hit
TGTCAGTTTGTCAAATACCCCA80410.10493619002676846No Hit
CAACGGAATCCCAAAAGCAGCTGA77520.10116469905329052No Hit
TTCACCACCTTCTCCACCCAGC77000.10048609168090003No Hit
TTCACAGTGGCTAAGTTCTG76960.10043389111379306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)