FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13123678
Sequences flagged as poor quality0
Sequence length18-26
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT215608016.428930974990394No Hit
CAACGGAATCCCAAAAGCAGCTG11843209.024299438008155No Hit
CCCCCCACTGCTAAATTTGACTG10694788.149224630473256No Hit
TCCTGTCTGAGCGTCGCT5407464.120384544637563No Hit
CCCCCACTGCTAAATTTGACTG5062113.8572342296115463No Hit
AACCGGGCGGAAACACCA2442111.8608426692578104No Hit
TTCAAGTAATCCAGGATAGGCT2064141.5728365173238783No Hit
CGCCTGTCTGAGCGTCGCT1772341.3504903122432599No Hit
AACATTCAACGCTGTCGGTGAGT1331471.0145555232306065No Hit
TAGCAGCACGTAAATATTGGCG982010.7482734641919743No Hit
CAACGGAATCCCAAAAGCAGCTGT853030.6499930888276899No Hit
GGAATACCGGGTGCTGTAGGCTTT812680.6192471348352192No Hit
ACTGGACTTGGAGTCAGAAGGC702950.5356349035689538No Hit
AACATTCAACGCTGTCGGTGAG634140.48320295575676275No Hit
GGAATACCGGGTGCTGTAGGCTT545680.41579807124191864No Hit
AAGCTGCCAGTTGAAGAACTGT532680.40589231159130845No Hit
CCCATAAAGTAGAAAGCACT523610.3989811392812289No Hit
CTCCCACTGCTTCACTTGACTAG514270.3918642319630214No Hit
CCCCCCACTGCTAAATTTGACTGG507120.38641606415518576No Hit
CAACACCAGTCGATGGGCTGT487540.3714964661583437No Hit
CATTGCACTTGTCTCGGTCTGA469700.3579027159916603No Hit
CCCATAAAGTAGAAAGCACTAC467040.3558758451708431No Hit
TCGTACGACTCTTAGCGG455300.3469301822248306No Hit
CCCCCACTGCTAAATTTGACTGGCTT426940.32532038655626877No Hit
CACCCGGCTGTGTGCACATGTGC410930.3131210625557866No Hit
TACGCCTGTCTGAGCGTCGCT398880.3039391853411825No Hit
AGCAGCATTGTACAGGGCTATGA379850.2894386771757125No Hit
CTACGCCTGTCTGAGCGTCGCT369650.28166646575754145No Hit
CCCCCCACTGCTAAATTTGACTGGCT368420.2807292284982914No Hit
CGCGACCTCAGATCAGACGT362840.2764773716636449No Hit
CATAAAGTAGAAAGCACTACT341540.260247165466876No Hit
CGCGACCTCAGATCAGACGTGGC310290.23643524322983236No Hit
ACCGGGTGCTGTAGGCTT308610.23515511429036892No Hit
ACGCCTGTCTGAGCGTCGCT299450.2281753636442467No Hit
CTTCTCACTACTGCACTTGACTAG276760.21088600314637407No Hit
TGTAAACATCCTACACTCTCAGCT251590.19170692849976964No Hit
GCCCCCACTGCTAAATTTGACTG238980.1820983416386778No Hit
AACGGAATCCCAAAAGCAGCTG234360.1785779870551533No Hit
TAGCTTATCAGACTGATGTTGAC233640.1780293603668118No Hit
TAGCTTATCAGACTGATGTTGA233280.17775504702264106No Hit
TTCTCACTACTGCACTTGACTAG229380.17478331912745804No Hit
GCCTGTCTGAGCGTCGCTT228090.17380036297751286No Hit
CCCTGTCTGAGCGTCGCT223510.17031048765445175No Hit
CGCGACCTCAGATCAGACGTGGCG221240.1685807896231529No Hit
AGCTCGGTCTGAGGCCCCTCAGT220460.16798644404411628No Hit
ACTGGACTTGGAGTCAGAAGGCT219120.16696538881859185No Hit
TGAATACCGGGTGCTGTAGGCTTT212260.16173819564911607No Hit
GGGGATGTAGCTCAGTGG210840.16065618190266479No Hit
TTCAAGTAATTCAGGATAGGTT209720.15980276260968915No Hit
ACCGGGTGCTGTAGGCTTA206650.15746347936912197No Hit
ACCGGGTGCTGTAGGCTTT199190.15177909729269493No Hit
GCCTGTCTGAGCGTCGCA195270.14879212976728018No Hit
ACTGGACTTGGAGTCAGAAGG194160.1479463302894204No Hit
GAACGGAATCCCAAAAGCAGCTG193480.14742818286154233No Hit
TCAAACGAGAACTTTGAAGGCCGAA187260.14268865785948118No Hit
GCGTTCGCGCTTTCCCCTG184590.14065416722354815No Hit
CAACGGAATCCCAAAAGCAGCT182620.13915306364572494No Hit
ATCCCGGACGAGCCCCCA180760.13773577803417608No Hit
TGAGGTAGTAGGTTGTATAGTT175390.13364393731696253No Hit
GCCGGTCTGAGCGTCGCT170700.130070244027627No Hit
CAAAGAATTCTCCTTTTGGGCT161790.12328098875940113No Hit
TCCTTCTGATCGAGGCCC158220.12056071476304127No Hit
CACTAGATTGTGAGCTCCTGGA157950.12035497975491322No Hit
CCCCCACTGCTAAATTTGACTGG154960.11807665503527288No Hit
AACATTCAACGCTGTCGGTGAGTT150150.11441152396454712No Hit
GCCTGTCTGAGCGTCGCG146970.11198842275770557No Hit
ACCACAGGGTAGAACCACGGAC143740.10952722247528474No Hit
TACCACCCTGAACGCGCCCGA141770.10802611889746151No Hit
TGAATACCGGGTGCTGTAGGCTT141520.10783562351956517No Hit
ATAGGCGATAGAAATTGAAACCT140080.1067383701428822No Hit
CCCCCCACTGCTAAATTTGACTGT139270.10612116511849803No Hit
CGCGACCTCAGATCAGACGTGG137430.1047191191371809No Hit
ACTGGACTTGGAGTCAGAAGGCA136460.10397999707094308No Hit
AGCCTCTGGCATGTTGGAAC136300.1038580800290894No Hit
CCCATAAAGTAGAAAGCACTA134280.10231887737568691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)