FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8569517
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG128951415.047685884747064No Hit
CCCCCCACTGCTAAATTTGACTG7115688.303478480759184No Hit
CCCCCACTGCTAAATTTGACTG3217433.754505650668526No Hit
TTCAAGTAATCCAGGATAGGCT3062123.5732702321496066No Hit
TAGCAGCACGTAAATATTGGCG2256442.63310055864292No Hit
AACATTCAACGCTGTCGGTGAG1775522.07190206869302No Hit
TAGCTTATCAGACTGATGTTGAC1610591.8794408132920444No Hit
TCGTACGACTCTTAGCGG1344661.5691199398985964No Hit
AAGCTGCCAGTTGAAGAACTGT1201451.4020043370005568No Hit
TGAGGTAGTAGATTGTATAGTT1184751.3825166575899202No Hit
TAGCTTATCAGACTGATGTTGACT902451.0530931906664052No Hit
TATTGCACTTGTCCCGGCCTGT900521.0508410217285291No Hit
TGAGGTAGTAGGTTGTATAGTT870591.0159148992877893No Hit
TAGCTTATCAGACTGATGTTGA852980.9953653163883098No Hit
TTAATGCTAATCGTGATAGGGGTT812920.9486182243409984No Hit
TATTGCACTTGTCCCGGCCTGTAA803670.9378241504159452No Hit
TATTGCACTTGTCCCGGCCTGTAT690470.8057280241115106No Hit
TGAGAACTGAATTCCATGGGTT684670.7989598480287746No Hit
AACATTCAACGCTGTCGGTGAGT636930.743250757306392No Hit
TGTAAACATCCTTGACTGGAAGCT634740.7406951873717036No Hit
CAACGGAATCCCAAAAGCAGCTGT618390.721615932379853No Hit
TTCTCACTACTGCACTTGACTAG559200.6525455285286207No Hit
TATTGCACTTGTCCCGGCCTGTA551710.6438052459666046No Hit
CCCCCACTGCTAAATTTGACTGGCTT531350.6200466140623795No Hit
AGCAGCATTGTACAGGGCTATGA473030.5519914366235577No Hit
TTCAAGTAATTCAGGATAGGTT440670.5142296817895338No Hit
CATAAAGTAGAAAGCACTACT410590.47912852031217157No Hit
TGAGGTAGTAGTTTGTACAGTT385430.4497686392360269No Hit
CACCCGGCTGTGTGCACATGTGC383460.4474697932217183No Hit
CAACGGAATCCCAAAAGCAGCT380380.44387565833640336No Hit
CATTGCACTTGTCTCGGTCTGA374790.4373525369049388No Hit
CTTCTCACTACTGCACTTGACTAG342950.4001975840645395No Hit
TGTAAACATCCCCGACTGGAAGCT333110.3887150232621045No Hit
ACTGGACTTGGAGTCAGAAGGC332440.3879331822318574No Hit
CCCATAAAGTAGAAAGCACTAC321660.3753537101332549No Hit
CTCCCACTGCTTCACTTGACTAG302000.3524119270666013No Hit
CTCCCACTGCTTCACTTGACTAGCCT284630.33214240662571765No Hit
AACGGAATCCCAAAAGCAGCTG269040.31395001608608747No Hit
TGAGAACTGAATTCCATAGGCTGT241720.28206957288257906No Hit
TAGCTTATCAGACTGATGTTGACA240780.2809726615864115No Hit
CCCCCCACTGCTAAATTTGACTGG237160.2767483861692555No Hit
CAAAGAATTCTCCTTTTGGGCT233060.2719639858349076No Hit
TAGCAGCACGTAAATATTGGC231420.27005022570116843No Hit
CCCATAAAGTAGAAAGCACT223880.2612515967936116No Hit
CCCCCCACTGCTAAATTTGACTGGCT200490.23395717634961222No Hit
ACCCCCCACTGCTAAATTTGACTG199410.23269689528593035No Hit
TGTCAGTTTGTCAAATACCCCA190770.22261464677647527No Hit
TCGTTTCCCGGCCAATGCACCA188740.22024578514751764No Hit
GGAATACCGGGTGCTGTAGGCTTT164300.1917260914471609No Hit
TGTAAACATCCTACACTCTCAGCT160620.18743180041535598No Hit
TGATGTAGTAGATTGTATAGTT158700.18519130074658816No Hit
TTCACAGTGGCTAAGTTCCGC156280.18236733762241208No Hit
TATTGCACTTGTCCCGGCCTGTT149400.17433888047599416No Hit
CAAAGTGCTGTTCGTGCAGGTAG139260.16250624160031424No Hit
CCCATAAAGTAGAAAGCACTA139070.16228452548725908No Hit
TTCACAGTGGCTAAGTTCTGC138810.1619811244904468No Hit
GCCTGTCTGAGCGTCGCT138420.16152602299522834No Hit
ACCGGGTGCTGTAGGCTT138110.1611642756528752No Hit
CAACACCAGTCGATGGGCTGT135050.1575934793057765No Hit
TTAATGCTAATCGTGATAGGGGTTT134860.15737176319272136No Hit
GGAATACCGGGTGCTGTAGGCTT134100.15648489874050078No Hit
CACTAGATTGTGAGCTCCTGGA132910.15509625571662908No Hit
CAAAGAATTCTCCTTTTGGGCTT120910.14109313278683036No Hit
ACTGGACTTGGAGTCAGAAGGCT119560.13951778145722799No Hit
AGCTCGGTCTGAGGCCCCTCAGT118920.13877094823430539No Hit
CACTAGATTGTGAGCTCCTGGAA118620.13842087016106042No Hit
TTCAAGTAATCCAGGATAGGC116810.13630873245248243No Hit
TGATGTAGTAGGTTGTATAGTT116730.13621537829961713No Hit
TACCACCCTGAACGCGCCCGA114180.13323971467703488No Hit
AATGCACCCGGGCAAGGATTCT112340.1310925691611324No Hit
TGAGGTAGTAGATTGTATAGT110840.12934217879490756No Hit
ATGCACCTGGGCAAGGATTCTGA110350.12877038460860746No Hit
TGAGGTAGTAAGTTGTATTGTT106320.12406766915801673No Hit
TTCAAGTAATCCAGGATAGGCTT103170.12039184938894457No Hit
TGAGAACTGAATTCCATGGGTTG100530.11731116234438885No Hit
ATCACATTGCCAGGGATTTCC99310.11588751151319263No Hit
ACCGGGTGCTGTAGGCTTT99000.1155257641708395No Hit
TCCCACTGCTTCACTTGACTAGCCT98320.11473225387148424No Hit
TTAATGCTAATCGTGATAGGGGT98010.11437050652913111No Hit
ACTGGACTTGGAGTCAGAAGGCA97700.11400875918677797No Hit
TATTGCACTTGTCCCGGCCTGTAG97540.11382205088104733No Hit
TTCACAGTGGCTAAGTTCTG97360.11361200403710034No Hit
TGTAAACATCCCCGACTGGA96330.11241006931895928No Hit
AGCTACATTGTCTGCTGGGTTT95840.11183827513265916No Hit
AATCCTTGGAACCTAGGTGTGAGT95570.11152320486673871No Hit
CAAAGAATTCTCCTTTTGGGC92190.10757899190817873No Hit
TGAGGTAGTAGTTTGTGCTGTT89580.1045333126709475No Hit
TCCCACTGCTTCACTTGACTAG88580.10336638576013094No Hit
AGCAGCATTGTACAGGGCTATGT86520.10096251632384881No Hit
TTCACAGTGGCTAAGTTCCG86080.10044906848308954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)