FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4714475
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG54286011.5147497865616No Hit
AACATTCAACGCTGTCGGTGAGT1726793.6627408141945814No Hit
CCCCCACTGCTAAATTTGACTG1458033.092666733835687No Hit
TAGCTTATCAGACTGATGTTGAC1280902.716951516340632No Hit
CAACGGAATCCCAAAAGCAGCTG1116742.3687473154486978No Hit
AAGCTGCCAGTTGAAGAACTGT1071782.2733814475630902No Hit
TAGCAGCACGTAAATATTGGCG910661.9316254726135997No Hit
TAGCTTATCAGACTGATGTTGACT832931.7667502744208No Hit
TTCAAGTAATCCAGGATAGGCT781411.6574698137120252No Hit
TATTGCACTTGTCCCGGCCTGTAA600601.2739488490234863No Hit
AACATTCAACGCTGTCGGTGAG559771.1873432354610005No Hit
TATTGCACTTGTCCCGGCCTGTAT527561.119021736248469No Hit
TAGCTTATCAGACTGATGTTGA480451.0190954454101464No Hit
TATTGCACTTGTCCCGGCCTGT472271.0017446269202828No Hit
CATAAAGTAGAAAGCACTACT459120.9738518074653063No Hit
CGCCTGTCTGAGCGTCGCT423450.898191208989336No Hit
TCCACCACGTTCCCGTGG361300.7663631687515577No Hit
ACTGGACTTGGAGTCAGAAGGC341800.725001193133912No Hit
TGAGGTAGTAGGTTGTATAGTT328620.6970447398702931No Hit
TGTAAACATCCTTGACTGGAAGCT314770.6676671315469909No Hit
CTCCCACTGCTTCACTTGACTAG263690.5593199666983067No Hit
TATTGCACTTGTCCCGGCCTGTA260100.5517051209307505No Hit
TGAGGTAGTAGATTGTATAGTT254810.5404843593401174No Hit
CTTCTCACTACTGCACTTGACTAG253830.5384056549244614No Hit
AGCAGCATTGTACAGGGCTATGA237600.5039797644488517No Hit
CTACGCCTGTCTGAGCGTCGCT212220.45014556233726977No Hit
TTCTCACTACTGCACTTGACTAG195880.4154863478966375No Hit
TACGCCTGTCTGAGCGTCGCT182300.3866814438511181No Hit
TGTAAACATCCCCGACTGGAAGCT181590.38517544371324486No Hit
CCCCCCACTGCTAAATTTGACTGGCT170780.3622460613323859No Hit
AACATTCAACGCTGTCGGTGAGTT169350.3592128497870919No Hit
GCGTTCGCGCTTTCCCCTG167410.3550978634948748No Hit
TCGTACGACTCTTAGCGG161150.3418196087581332No Hit
ACCGGGTGCTGTAGGCTT156030.33095943874980777No Hit
GTCAGGATGGCCGAGCGGTCTAAGG133290.2827250117987687No Hit
TGTAAACATCCTACACTCTCAGCT132490.2810281102349678No Hit
AGCTCGGTCTGAGGCCCCTCAGT131360.27863123677609913No Hit
CCCCCCACTGCTAAATTTGACTGG127280.26997703880071483No Hit
TAGCTTATCAGACTGATGTTGACA121710.2581623616627514No Hit
TGAGGTAGTAGTTTGTGCTGTT120840.256316981212118No Hit
GCCTGTCTGAGCGTCGCT120840.256316981212118No Hit
CCCCCACTGCTAAATTTGACTGGCTT109190.23160585218926816No Hit
ACCGGGTGCTGTAGGCTTT105780.22437280927356706No Hit
CATTGCACTTGTCTCGGTCTGA100430.21302478006564887No Hit
CCAAGTCCTTCTGATCGAGGCCC100010.21213390674465343No Hit
GGAATACCGGGTGCTGTAGGCTTT93320.19794356741736885No Hit
TAGCAGCACATCATGGTTTACA90450.1918559330572333No Hit
TCTCCCAACCCTTGTACCAGTG89000.18878029897284426No Hit
CTCCCACTGCTTCACTTGACTAGCCT88910.18858939754691667No Hit
ACGCCTGTCTGAGCGTCGCT87030.18460167887198467No Hit
ACCGGGTGCTGTAGGCTTA86440.18335021396868156No Hit
CACTAGATTGTGAGCTCCTGGA84270.17874736847687175No Hit
CGCGACCTCAGATCAGACGTGGCG83760.17766559372994872No Hit
TTCAAGTAATTCAGGATAGGTT83500.17711410072171344No Hit
ACTGGACTTGGAGTCAGAAGGCA82220.17439905821963209No Hit
CCCATAAAGTAGAAAGCACTAC80640.17104767763112544No Hit
TATTGCACTTGTCCCGGCCTGTT80180.17007195923193993No Hit
CAAAGAATTCTCCTTTTGGGCT79100.1677811421208088No Hit
TCGTTCGCGCTTTCCCCTG78090.16563880389651023No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC78000.16544790247058264No Hit
TGAGGTAGTAGTTTGTACAGTT76990.16330556424628406No Hit
TATTGCACTTGTCCCGGCCTGTAG76300.16184198664750582No Hit
CCCCCCACTGCTAAATTTGACTGT73130.15511801420094495No Hit
CCTCGACACAAGGGTTTGT70960.15051516870913517No Hit
CTGCGTTCGCGCTTTCCCCTG70860.15030305601366006No Hit
GGAATACCGGGTGCTGTAGGCTT70670.14990004189225736No Hit
CACTAGATTGTGAGCTCCTGGAA69350.14710015431198598No Hit
TTAATGCTAATCGTGATAGGGGTT69200.14678198526877329No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT68790.14591232321732536No Hit
CAGTGCAATAGTATTGTCAAAGC68640.1455941541741127No Hit
AACATTCAACGCTGTCGGTGA68330.14493660481813989No Hit
ACCCCCCACTGCTAAATTTGACTG68170.1445972245053797No Hit
TTCACAGTGGCTAAGTTCCG68150.1445548019662847No Hit
CAAGTCCTTCTGATCGAGGCCC66680.14143674534280062No Hit
CAACGGAATCCCAAAAGCAGCTGT66500.14105494249094544No Hit
GGGCTACGCCTGTCTGAGCGTCGCT65360.13863685776252924No Hit
CCCATAAAGTAGAAAGCACT64620.13706722381601344No Hit
CCGATGGTAGTGGGTTATCAGAACT64410.13662178715551573No Hit
CAAAGAATTCTCCTTTTGGGCTT64250.13628240684275555No Hit
ACTGGACTTGGAGTCAGAAGGCT63090.13382189957524435No Hit
TGTAAACATCCTACACTCAGCT62150.13182804023777833No Hit
TCAGGCTCAGTCCCCTCCCGAT61860.13121291342090052No Hit
TTATAAAGCAATGAGACTGATT61660.1307886880299503No Hit
AACCGGGCGGAAACACCA60620.1285827159970092No Hit
ACTGGACTTGGAGTCAGAAGG60500.12832818076243907No Hit
TTCAAGTAATCCAGGATAGGCTT60320.12794637791058389No Hit
CCCCCCACTGCTAAATTTGACTGA60080.12743730744144363No Hit
TGAGGTAGTAGGTTGTGTGGTT58230.12351322257515417No Hit
CCCCCCACTGCTAAATTTGACTGTA57070.12105271530764294No Hit
TTCAGGATGGCCGAGCGGTCTAAGG56620.12009820817800497No Hit
TCCTGTCTGAGCGTCGCT56280.1193770250133896No Hit
CACCCGGCTGTGTGCACATGTGC55440.11759527837139873No Hit
TCCCACTGCTTCACTTGACTAG55170.11702257409361594No Hit
GAATACCGGGTGCTGTAGGCTTT54470.1155377852252902No Hit
AACATTCAACGCTGTCGGTGAGA53600.11369240477465677No Hit
TATTGCACTTGTCCCGGCCTGTTT53100.11263184129728125No Hit
CCCCCACTGCTAAATTTGACTGGCT52790.11197429194130842No Hit
AACATTCATTGCTGTCGGTGGGT51380.10898350293510942No Hit
TGTAAACATCCCCGACTGGAAGC49660.10533516457293761No Hit
TGAGGGGCAGAGAGCGAGACTTT48740.10338372777456663No Hit
CATAAAGTAGAAAGCACTACTA47390.1005202063856527No Hit
GTCAGGATGGCCGAGTGGTCTAAGG47360.10045657257701016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)