FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6155690
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3305895.370462125285711No Hit
AACATTCAACGCTGTCGGTGAGT1912743.107271483781672No Hit
TAGCAGCACGTAAATATTGGCG1811252.9423996335098095No Hit
CCCCCCACTGCTAAATTTGACTG1745672.8358640542327507No Hit
AAGCTGCCAGTTGAAGAACTGT1720682.795267467984905No Hit
TAGCTTATCAGACTGATGTTGAC1506992.448125230477818No Hit
TTCAAGTAATCCAGGATAGGCT1463312.3771664914899873No Hit
CGCCTGTCTGAGCGTCGCT1330192.160911286955646No Hit
AACATTCAACGCTGTCGGTGAG1096551.7813600100070017No Hit
TATTGCACTTGTCCCGGCCTGTAA945401.5358148314811173No Hit
TAGCTTATCAGACTGATGTTGACT869941.4132290612425251No Hit
TGAGGTAGTAGGTTGTATAGTT794301.2903508786179942No Hit
TATTGCACTTGTCCCGGCCTGTAT766031.2444258888930404No Hit
GCCTGTCTGAGCGTCGCT663071.0771660041360107No Hit
TAGCTTATCAGACTGATGTTGA660231.0725523864911977No Hit
TATTGCACTTGTCCCGGCCTGT610040.9910180662119112No Hit
AGCAGCATTGTACAGGGCTATGA577110.9375228447176513No Hit
ACTGGACTTGGAGTCAGAAGGC573670.9319345191197087No Hit
TGTAAACATCCTTGACTGGAAGCT544590.8846936736580302No Hit
TGAGGTAGTAGATTGTATAGTT528250.8581491270678023No Hit
CCCCCACTGCTAAATTTGACTG526320.8550138164852357No Hit
TATTGCACTTGTCCCGGCCTGTA442360.7186196835773082No Hit
TACGCCTGTCTGAGCGTCGCT396370.6439083189699286No Hit
CATAAAGTAGAAAGCACTACT360020.5848572621428304No Hit
TTCAAGTAATTCAGGATAGGTT337300.5479483209843251No Hit
GCGTTCGCGCTTTCCCCTG305530.4963375348661157No Hit
TGTAAACATCCCCGACTGGAAGCT284450.46209279544616444No Hit
CTACGCCTGTCTGAGCGTCGCT260300.4228608003327003No Hit
TCCTGTCTGAGCGTCGCT258450.4198554508105509No Hit
TCGTACGACTCTTAGCGG228100.37055147351474815No Hit
ACGCCTGTCTGAGCGTCGCT225370.3661165523280087No Hit
CATTGCACTTGTCTCGGTCTGA223850.3636472921800805No Hit
TGAGGTAGTAGTTTGTACAGTT211460.3435195729479555No Hit
CTCGTACGACTCTTAGCGG200500.32571490767078914No Hit
CAACGGAATCCCAAAAGCAGCTGT181210.29437804697767433No Hit
TGTCAGTTTGTCAAATACCCCAA181140.2942643310498092No Hit
AACATTCAACGCTGTCGGTGAGTT179150.29103154967192957No Hit
TTATAAAGCAATGAGACTGATT169140.2747701719872183No Hit
CCCATAAAGTAGAAAGCACTAC169120.27473768172211405No Hit
CACCCGGCTGTGTGCACATGTGC168240.273308110057524No Hit
CAACACCAGTCGATGGGCTGT162890.2646169641421189No Hit
TAGCTTATCAGACTGATGTTGACA156780.25469118815274977No Hit
ACCGGGTGCTGTAGGCTT156550.25431755010405005No Hit
TGAGGTAGTAGTTTGTGCTGTT149670.24314089890816465No Hit
TGTAAACATCCTACACTCTCAGCT140580.22837407341825205No Hit
AACCGGGCGGAAACACCA139610.22679829556069264No Hit
TCGTTCGCGCTTTCCCCTG139570.226733315030484No Hit
CCTCGACACAAGGGTTTGT134450.21841580716377854No Hit
ACTGGACTTGGAGTCAGAAGGCA133130.21627144966689357No Hit
CCCATAAAGTAGAAAGCACT128000.20793769666763595No Hit
CAACGGAATCCCAAAAGCAGCT123020.19984762065666076No Hit
CTTCTCACTACTGCACTTGACTAG122370.1987916870407704No Hit
TTCTCACTACTGCACTTGACTAG117230.19044168890896065No Hit
CTCCCACTGCTTCACTTGACTAG113820.1849020987086744No Hit
AGCAGCATTGTACAGGGCTATGT110310.17920005718286658No Hit
ACCGGGTGCTGTAGGCTTT109410.17773799525317227No Hit
CCCCCACTGCTAAATTTGACTGGCTT108620.17645462978155171No Hit
ACCACAGGGTAGAACCACGGAC104980.1705414015325658No Hit
TAGCAGCACGTAAATATTGGC104920.17044393073725286No Hit
ACCGGGTGCTGTAGGCTTA104080.1690793396028715No Hit
CTGCGTTCGCGCTTTCCCCTG103890.16877068208438045No Hit
ACTGGACTTGGAGTCAGAAGGCT103680.1684295343007851No Hit
ACTGGACTTGGAGTCAGAAGG101750.16529422371821845No Hit
TATTGCACTTGTCCCGGCCTGTAG101230.16444947682550615No Hit
TGTCAGTTTGTCAAATACCCCA100400.16310113082367697No Hit
CGCGACCTCAGATCAGACGTGGCG98860.16059938041064448No Hit
TAGCAGCACGTAAATATTGGCGT97150.15782146274422526No Hit
TGAGGTAGTAGGTTGTGTGGTT96510.15678177426088707No Hit
AACGGAATCCCAAAAGCAGCTG95250.15473488755931503No Hit
CCCCCCACTGCTAAATTTGACTGGCT94680.15380891500384197No Hit
TTCAAGTAATCCAGGATAGGCTT94100.15286669731581676No Hit
GGAATACCGGGTGCTGTAGGCTTT92280.1499100831913238No Hit
AGCAGCATTGTACAGGGCTATG91160.14809062834548198No Hit
TATTGCACTTGTCCCGGCCTGTT90780.14747331330849994No Hit
CCAAGTCCTTCTGATCGAGGCCC86420.14039043551575858No Hit
TACCTGGTTGATCCTGCC85520.13892837358606427No Hit
TGTAAACATCCTACACTCAGCT83800.13613421078709292No Hit
CAAAGTGCTGTTCGTGCAGGTAG83000.1348346001829202No Hit
CTCCCACTGCTTCACTTGACTAGCCT82450.13394111789255145No Hit
CACTAGATTGTGAGCTCCTGGA80700.1310982196959236No Hit
TTCACAGTGGCTAAGTTCCG80130.13017224714045053No Hit
CTCGACACAAGGGTTTGT80090.1301072666102419No Hit
CAAGTCCTTCTGATCGAGGCCC78550.12760551619720942No Hit
ATCCCACCGCTGCCACCA77640.12612720913496295No Hit
ATAGGCGATAGAAATTGAAACCT75310.12234209325030988No Hit
CAAAGAATTCTCCTTTTGGGCTT75240.12222837732244476No Hit
GGGCTACGCCTGTCTGAGCGTCGCT74630.12123742423676305No Hit
GGAATACCGGGTGCTGTAGGCTT74490.12100999238103284No Hit
TGAGGTAGTAGGTTGTATAGT73690.11971038177686011No Hit
TGTAAACATCCCCGACTGGAAGC73550.11948294992112987No Hit
CACTAGATTGTGAGCTCCTGGAA71740.1165425809291891No Hit
TTCACAGTGGCTAAGTTCCGC71680.11644511013387614No Hit
CAAAGAATTCTCCTTTTGGGCT70440.11443071369740843No Hit
TTCACAGTGGCTAAGTTCTGC69120.11228635620052342No Hit
TAGTGGGTTATCAGAACT69060.11218888540521045No Hit
CCCCCCACTGCTAAATTTGACTGG68320.1109867455963507No Hit
TATTGCACTTGTCCCGGCCTGTAAT65910.10707166865128036No Hit
AGAGGTAGTAGGTTGCATAGTT65450.10632439255388104No Hit
ATCCCGGACGAGCCCCCA64940.10549589079372093No Hit
TTCAAGTAATCCAGGATAGGC63850.10372517134553559No Hit
CAAAGTGCTGTTCGTGCAGGT62690.10184073596948515No Hit
GAATACCGGGTGCTGTAGGCTTT62420.10140211739057683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)