FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6990203
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4865326.960198437727774No Hit
CCCCCCACTGCTAAATTTGACTG3232874.624858534151297No Hit
AACATTCAACGCTGTCGGTGAGT2296653.2855269010070236No Hit
TAGCAGCACGTAAATATTGGCG2171303.106204497923737No Hit
TTCAAGTAATCCAGGATAGGCT1990672.847799985207869No Hit
AAGCTGCCAGTTGAAGAACTGT1927342.7572017579460857No Hit
TAGCTTATCAGACTGATGTTGAC1482372.1206394149068344No Hit
AACATTCAACGCTGTCGGTGAG1260931.803853192818578No Hit
TATTGCACTTGTCCCGGCCTGTAA1241801.7764863194960148No Hit
TAGCTTATCAGACTGATGTTGACT1099091.5723291583949708No Hit
TATTGCACTTGTCCCGGCCTGTAT962731.3772561397716203No Hit
TGTAAACATCCTTGACTGGAAGCT859991.2302790062033964No Hit
CCCCCACTGCTAAATTTGACTG751751.0754337177332332No Hit
CGCCTGTCTGAGCGTCGCT682300.9760803799260193No Hit
TGAGGTAGTAGGTTGTATAGTT646680.9251233476338241No Hit
ACTGGACTTGGAGTCAGAAGGC637170.9115185925215619No Hit
TAGCTTATCAGACTGATGTTGA601560.8605758659655521No Hit
AGCAGCATTGTACAGGGCTATGA591610.8463416584611348No Hit
TATTGCACTTGTCCCGGCCTGT558790.7993902323008358No Hit
TGTAAACATCCCCGACTGGAAGCT498160.7126545538090954No Hit
TATTGCACTTGTCCCGGCCTGTA433610.6203110267327No Hit
TGAGGTAGTAGATTGTATAGTT378690.541743923602791No Hit
CAACGGAATCCCAAAAGCAGCTGT322980.4620466673142396No Hit
GCCTGTCTGAGCGTCGCT322340.46113110019837766No Hit
TTCAAGTAATTCAGGATAGGTT299310.4281849897635305No Hit
TACGCCTGTCTGAGCGTCGCT290480.4155530247118717No Hit
CATAAAGTAGAAAGCACTACT283400.40542456349264816No Hit
CCCCCACTGCTAAATTTGACTGGCTT279080.3992444854605796No Hit
CTACGCCTGTCTGAGCGTCGCT252530.3612627558884914No Hit
CCCCCCACTGCTAAATTTGACTGGCT251360.35958898475480616No Hit
GCGTTCGCGCTTTCCCCTG242860.34742910899726376No Hit
AACATTCAACGCTGTCGGTGAGTT231020.3304911173538165No Hit
CCCATAAAGTAGAAAGCACTAC219930.31462605592427No Hit
CACCCGGCTGTGTGCACATGTGC214400.30671498381377477No Hit
TGTAAACATCCTACACTCTCAGCT204960.29321036885481006No Hit
AACCGGGCGGAAACACCA200240.2864580613753277No Hit
TAGCTTATCAGACTGATGTTGACA192560.27547125598498357No Hit
CGCGACCTCAGATCAGACGTGGCG189320.2708361974609321No Hit
CTTCTCACTACTGCACTTGACTAG181340.2594202199850276No Hit
CATTGCACTTGTCTCGGTCTGA175500.25106567005278674No Hit
TGTCAGTTTGTCAAATACCCCAA175030.2503933004520756No Hit
CTCCCACTGCTTCACTTGACTAG173180.24774673925778692No Hit
TGAGGTAGTAGTTTGTACAGTT165420.23664548797795998No Hit
ACTGGACTTGGAGTCAGAAGGCA161180.23057985583537416No Hit
TTAATGCTAATCGTGATAGGGGTT159110.22761856844500794No Hit
ACGCCTGTCTGAGCGTCGCT158970.22741828813841314No Hit
CTCCCACTGCTTCACTTGACTAGCCT158610.22690328163574078No Hit
TTCTCACTACTGCACTTGACTAG156960.224542835165159No Hit
CAAAGAATTCTCCTTTTGGGCT156360.2236844909940384No Hit
GGAATACCGGGTGCTGTAGGCTTT153050.21894929231668953No Hit
CAACGGAATCCCAAAAGCAGCT150630.21548730415983627No Hit
CAAAGAATTCTCCTTTTGGGCTT138890.19869236987824246No Hit
TATTGCACTTGTCCCGGCCTGTAG136060.1946438465377901No Hit
CCCCCCACTGCTAAATTTGACTGG135550.19391425399233755No Hit
TTCAAGTAATCCAGGATAGGCTT128430.18372856982837266No Hit
CCAAGTCCTTCTGATCGAGGCCC127550.1824696650440624No Hit
CCCATAAAGTAGAAAGCACT126930.18158270940057106No Hit
TGTAAACATCCCCGACTGGAAGC123750.17703348529363166No Hit
CACTAGATTGTGAGCTCCTGGAA123240.17630389274817912No Hit
TAGCAGCACGTAAATATTGGC123060.17604638949684293No Hit
ACTGGACTTGGAGTCAGAAGGCT122680.17550277152179988No Hit
TGAGGTAGTAGTTTGTGCTGTT121810.17425817247367492No Hit
CACTAGATTGTGAGCTCCTGGA116970.16733419615996845No Hit
AACGGAATCCCAAAAGCAGCTG116520.16669043803162797No Hit
TAGCAGCACGTAAATATTGGCGT113140.16185509920098173No Hit
GGAATACCGGGTGCTGTAGGCTT110190.15763490702630525No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT106680.1526135936252495No Hit
TGTCAGTTTGTCAAATACCCCA104720.14980966933292209No Hit
AGCAGCATTGTACAGGGCTATGT104700.1497810578605514No Hit
GGGCTACGCCTGTCTGAGCGTCGCT103430.14796422936501272No Hit
ACTGGACTTGGAGTCAGAAGG99440.14225624062706047No Hit
TATTGCACTTGTCCCGGCCTGTAAT99360.1421417947375777No Hit
TATTGCACTTGTCCCGGCCTGTT98710.14121192188553036No Hit
CAACACCAGTCGATGGGCTGT97740.13982426547555202No Hit
ACCACAGGGTAGAACCACGGAC96400.1379072968267159No Hit
CTGCGTTCGCGCTTTCCCCTG95610.13677714366807375No Hit
AGCTCGGTCTGAGGCCCCTCAGT95600.1367628379318884No Hit
TCGTACGACTCTTAGCGG93850.13425933409945318No Hit
ACCCCCCACTGCTAAATTTGACTG91910.13148402127949646No Hit
CCTCGACACAAGGGTTTGT91670.1311406836110482No Hit
TTATAAAGCAATGAGACTGATT91110.13033956238466896No Hit
CAAAGTGCTGTTCGTGCAGGTAG90310.12919510348984142No Hit
TATTGCACTTGTCCCGGCCTGTAAA89520.12806495033119925No Hit
TGAGGTAGTAGGTTGTGTGGTT89040.12737827499430274No Hit
TTCAAGTAATCCAGGATAGGC84870.12141278300501429No Hit
CAAGTCCTTCTGATCGAGGCCC84620.12105513960038071No Hit
GTCAGGATGGCCGAGCGGTCTAAGG83780.1198534577608118No Hit
TGTAAACATCCTACACTCAGCT81880.11713536788559645No Hit
ATCACATTGCCAGGGATTTCCA81490.11657744417436804No Hit
ATAGGCGATAGAAATTGAAACCT79090.1131440674898855No Hit
ACCGGGTGCTGTAGGCTT78780.11270058966813984No Hit
AACATTCAACGCTGTCGGTGAGA77920.11147029635620025No Hit
TTCACAGTGGCTAAGTTCCG77880.11141307341145887No Hit
TCCTGTCTGAGCGTCGCT76390.10928151871984261No Hit
GAATACCGGGTGCTGTAGGCTTT75150.10750760743285996No Hit
ATGCACCTGGGCAAGGATTCTGA74210.10616286823143763No Hit
AGCAGCATTGTACAGGGCTATG71650.10250059976798957No Hit
CCCCCACTGCTAAATTTGACTGGCT70970.10152780970738619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)