FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5569352
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4403827.907239477770483No Hit
AAGCTGCCAGTTGAAGAACTGT2091143.754727659519456No Hit
CCCCCCACTGCTAAATTTGACTG1980093.555332828666603No Hit
AACATTCAACGCTGTCGGTGAGT1949913.5011434005248727No Hit
TAGCAGCACGTAAATATTGGCG1775703.1883421985178884No Hit
TTCAAGTAATCCAGGATAGGCT1708633.0679152619550716No Hit
AACATTCAACGCTGTCGGTGAG1029671.8488147274584188No Hit
TAGCTTATCAGACTGATGTTGAC949021.7040043437728483No Hit
TATTGCACTTGTCCCGGCCTGTAA853621.532709729964994No Hit
GCCTGTCTGAGCGTCGCT726701.3048196630415891No Hit
TGAGGTAGTAGGTTGTATAGTT706341.2682624477677116No Hit
TATTGCACTTGTCCCGGCCTGTAT689541.238097358543687No Hit
TAGCTTATCAGACTGATGTTGACT670151.203281818064292No Hit
CGCCTGTCTGAGCGTCGCT609811.0949388726013367No Hit
TAGCTTATCAGACTGATGTTGA594441.0673414070434046No Hit
CCCCCACTGCTAAATTTGACTG592521.0638939682749446No Hit
TATTGCACTTGTCCCGGCCTGT588771.0571606894302965No Hit
TGTAAACATCCTTGACTGGAAGCT582231.0454178511252297No Hit
AGCAGCATTGTACAGGGCTATGA489980.8797791915468801No Hit
TGAGGTAGTAGATTGTATAGTT446700.802068175974512No Hit
CATAAAGTAGAAAGCACTACT380320.6828801627191098No Hit
ACTGGACTTGGAGTCAGAAGGC373520.6706704837474808No Hit
TATTGCACTTGTCCCGGCCTGTA365100.6555520283149638No Hit
TGTAAACATCCCCGACTGGAAGCT335390.6022065044550964No Hit
TTCAAGTAATTCAGGATAGGTT332670.5973226328664448No Hit
CAACGGAATCCCAAAAGCAGCTGT278880.5007404811188088No Hit
CCCATAAAGTAGAAAGCACTAC262800.4718681814329566No Hit
GGAATACCGGGTGCTGTAGGCTTT228780.4107838757543068No Hit
TTAATGCTAATCGTGATAGGGGTT208960.37519625263405865No Hit
CTACGCCTGTCTGAGCGTCGCT206010.36989940660960197No Hit
TGTAAACATCCTACACTCTCAGCT202500.36359705761101113No Hit
TTCTCACTACTGCACTTGACTAG202460.3635252359700015No Hit
TGAGGTAGTAGTTTGTACAGTT196390.3526263019467974No Hit
TACGCCTGTCTGAGCGTCGCT187260.3362330123863602No Hit
CATTGCACTTGTCTCGGTCTGA178920.32125820023586227No Hit
GGAATACCGGGTGCTGTAGGCTT176300.3165538827497346No Hit
CCCCCACTGCTAAATTTGACTGGCTT174870.313986259083642No Hit
CTCCCACTGCTTCACTTGACTAG173630.311759788212345No Hit
TGTCAGTTTGTCAAATACCCCAA173160.31091588393048236No Hit
CACCCGGCTGTGTGCACATGTGC165910.29789821149749557No Hit
CCCATAAAGTAGAAAGCACT165680.2974852370616905No Hit
CTTCTCACTACTGCACTTGACTAG163420.2934273143446491No Hit
AACATTCAACGCTGTCGGTGAGTT158140.28394685773138423No Hit
GCGTTCGCGCTTTCCCCTG152700.274179114554081No Hit
ACGCCTGTCTGAGCGTCGCT149700.2687924914783623No Hit
CCCCCCACTGCTAAATTTGACTGGCT149480.2683974724528096No Hit
CTCCCACTGCTTCACTTGACTAGCCT139080.24972384579031817No Hit
ACCGGGTGCTGTAGGCTT131290.23573658120370197No Hit
TGAGGTAGTAGTTTGTGCTGTT130950.23512609725512054No Hit
TGTCAGTTTGTCAAATACCCCA119580.21471079579814673No Hit
GGGCTACGCCTGTCTGAGCGTCGCT117310.21063491767085293No Hit
TAGCTTATCAGACTGATGTTGACA116960.21000647831201907No Hit
CAAAGAATTCTCCTTTTGGGCT111070.19943074167335806No Hit
CAACACCAGTCGATGGGCTGT106150.1905966798291794No Hit
AGCTCGGTCTGAGGCCCCTCAGT106040.19039917031640305No Hit
AACCGGGCGGAAACACCA104680.18795723452207724No Hit
TGATGTAGTAGGTTGTATAGTT103310.18549734331749904No Hit
AACGGAATCCCAAAAGCAGCTG102730.1844559295228601No Hit
TGTAAACATCCCCGACTGGAAGC101660.18253470062585378No Hit
TCGTACGACTCTTAGCGG101430.18212172619004868No Hit
CCCCCCACTGCTAAATTTGACTGG100670.1807571150108666No Hit
TGTAAACATCCTACACTCAGCT99660.17894361857537466No Hit
TAGCAGCACGTAAATATTGGC96460.17319788729460805No Hit
TATTGCACTTGTCCCGGCCTGTAG93330.16757784388560823No Hit
TGAGGTAGTAGGTTGTGTGGTT93120.16720078027030794No Hit
TATTGCACTTGTCCCGGCCTGTT92060.165297506783554No Hit
ACTGGACTTGGAGTCAGAAGGCA91910.16502817562976804No Hit
ATCCCGGACGAGCCCCCA90890.16319672378402372No Hit
CAAAGAATTCTCCTTTTGGGCTT90670.162801704758471No Hit
CAACGGAATCCCAAAAGCAGCT89870.16136527193827935No Hit
TTCAAGTAATCCAGGATAGGCTT89790.1612216286562602No Hit
AGCAGCATTGTACAGGGCTATGT89370.16046750142565958No Hit
ATCCCACCGCTGCCACCA84940.15251325468384833No Hit
TAGCAGCACGTAAATATTGGCGT81490.14631863814677182No Hit
TTATAAAGCAATGAGACTGATT80970.14538495681364727No Hit
ACCGGGTGCTGTAGGCTTT79850.14337395086537896No Hit
TTCACAGTGGCTAAGTTCCG79500.1427455115065451No Hit
ACCGGGTGCTGTAGGCTTA77780.13965718094313306No Hit
ACCACAGGGTAGAACCACGGAC76610.13755639794360278No Hit
TTCAAGTAATCCAGGATAGGC74060.1329777683292419No Hit
CAAAGTGCTGTTCGTGCAGGTAG74020.1329059466882323No Hit
ACTGGACTTGGAGTCAGAAGG73770.1324570614319224No Hit
TTCACAGTGGCTAAGTTCCGC71350.1281118521508427No Hit
AGCAGCATTGTACAGGGCTATG71130.12771683312528997No Hit
GAATACCGGGTGCTGTAGGCTTT69510.1248080566644019No Hit
CACTAGATTGTGAGCTCCTGGA68440.12288682776739555No Hit
TGAGGTAGTAGGTTGTATAGT67990.12207883430603776No Hit
ACTGGACTTGGAGTCAGAAGGCT67810.12175563692149464No Hit
GTCAGGATGGCCGAGCGGTCTAAGG65480.11757202633268646No Hit
TTCACAGTGGCTAAGTTCTGC65350.11733860599940531No Hit
TGAGAACTGAATTCCATGGGTT65250.11715905189688136No Hit
TGATGTAGTAGATTGTATAGTT65170.11701540861486219No Hit
AGAGGTAGTAGGTTGCATAGTT65040.11678198828158105No Hit
TTCACCACCTTCTCCACCCAGC64690.11615354892274721No Hit
AACATTCAACGCTGTCGGTGA63910.11475302692306034No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT63270.11360388066690703No Hit
AACATTCAACGCTGTCGGTGAGA63220.11351410361564505No Hit
CAAAGTGCTGTTCGTGCAGGT62660.11250860064151091No Hit
CACTAGATTGTGAGCTCCTGGAA61370.11019235271895186No Hit
TATTGCACTTGTCCCGGCCTGTAAT59310.10649353820695835No Hit
CGCGACCTCAGATCAGACGTGGCG59060.10604465295064847No Hit
ACCCCCCACTGCTAAATTTGACTG58920.10579327720711493No Hit
GACCCGCGGGCGCTCTCCAGTCCT57620.1034590738743035No Hit
CCAAGTCCTTCTGATCGAGGCCC57120.10256130336168373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)