FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908663
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG120015015.175131371762838No Hit
CCCCCCACTGCTAAATTTGACTG3775304.773626085723971No Hit
TTCAAGTAATCCAGGATAGGCT2948783.728544255836922No Hit
TAGCAGCACGTAAATATTGGCG2142062.708498263233621No Hit
AACATTCAACGCTGTCGGTGAG1934712.4463174116788133No Hit
TGAGGTAGTAGATTGTATAGTT1458821.8445848558726046No Hit
CCCCCACTGCTAAATTTGACTG1411011.7841321598859379No Hit
TAGCTTATCAGACTGATGTTGAC1401141.7716521743308573No Hit
AAGCTGCCAGTTGAAGAACTGT1225401.549440151894195No Hit
TATTGCACTTGTCCCGGCCTGTAA1071971.3554377016696755No Hit
TGAGGTAGTAGGTTGTATAGTT974491.2321804583151412No Hit
GCCTGTCTGAGCGTCGCT885821.1200628981156486No Hit
TATTGCACTTGTCCCGGCCTGT843151.0661094043329447No Hit
TAGCTTATCAGACTGATGTTGA830741.0504177507626764No Hit
TATTGCACTTGTCCCGGCCTGTAT809011.0229415515618758No Hit
TAGCTTATCAGACTGATGTTGACT802261.0144066070333253No Hit
AACATTCAACGCTGTCGGTGAGT692200.8752427559500259No Hit
CCCCCACTGCTAAATTTGACTGGCTT643870.8141325531256042No Hit
TGAGAACTGAATTCCATGGGTT569620.7202481633115484No Hit
CCCATAAAGTAGAAAGCACTAC556650.7038484254544669No Hit
TGTAAACATCCTTGACTGGAAGCT555600.7025207674166923No Hit
CAACGGAATCCCAAAAGCAGCTGT555020.7017873944053502No Hit
CATAAAGTAGAAAGCACTACT546950.691583394057883No Hit
TATTGCACTTGTCCCGGCCTGTA534230.6754997652574145No Hit
TGAGGTAGTAGTTTGTACAGTT494260.6249602492861309No Hit
TTAATGCTAATCGTGATAGGGGTT458670.5799589639867068No Hit
AGCAGCATTGTACAGGGCTATGA443400.5606510228087858No Hit
TTCAAGTAATTCAGGATAGGTT439730.5560105418577072No Hit
TTCTCACTACTGCACTTGACTAG355920.45003814171877093No Hit
CACCCGGCTGTGTGCACATGTGC348640.4408330459902009No Hit
ACTGGACTTGGAGTCAGAAGGC347830.43980885264677483No Hit
CTCCCACTGCTTCACTTGACTAG325860.4120291887516259No Hit
CATTGCACTTGTCTCGGTCTGA315420.3988284745474678No Hit
TGTAAACATCCCCGACTGGAAGCT314230.39732379543799No Hit
GGAATACCGGGTGCTGTAGGCTTT310420.3925062934152081No Hit
TCGTACGACTCTTAGCGG301920.3817585854903667No Hit
CTCCCACTGCTTCACTTGACTAGCCT298180.3770295940034365No Hit
TTCACAGTGGCTAAGTTCCGC275890.34884531051582296No Hit
AACGGAATCCCAAAAGCAGCTG270900.34253577374582783No Hit
CAACGGAATCCCAAAAGCAGCT264040.3338617412323676No Hit
TGAGAACTGAATTCCATAGGCTGT262750.3322306185002446No Hit
CCCATAAAGTAGAAAGCACT244070.30861094979012255No Hit
GGAATACCGGGTGCTGTAGGCTT233420.29514470397840953No Hit
CTTCTCACTACTGCACTTGACTAG229290.28992258236316304No Hit
CCCCCCACTGCTAAATTTGACTGGCT223180.2821968770195417No Hit
CAAAGAATTCTCCTTTTGGGCT211970.2680225469210156No Hit
TAGCTTATCAGACTGATGTTGACA207360.2621934959170722No Hit
TAGCAGCACGTAAATATTGGC199700.2525079144224504No Hit
ACCGGGTGCTGTAGGCTT190440.24079923496550557No Hit
TGTAAACATCCTACACTCTCAGCT175830.22232582169704287No Hit
TCCTGTCTGAGCGTCGCT172170.2176979851082288No Hit
TGATGTAGTAGATTGTATAGTT167310.21155282504767242No Hit
CCCCCCACTGCTAAATTTGACTGG157620.19930043801335318No Hit
TCGTTTCCCGGCCAATGCACCA152700.1930794117792097No Hit
TGTAAACATCCCCGACTGGA152120.1923460387678676No Hit
TGTCAGTTTGTCAAATACCCCA149480.1890079271300345No Hit
CCCATAAAGTAGAAAGCACTA149130.1885653744507763No Hit
TGAGGTAGTAGATTGTATAGT142070.1796384546920257No Hit
TTCACAGTGGCTAAGTTCTGC138790.17549110386926337No Hit
CAACACCAGTCGATGGGCTGT134880.17054715822383631No Hit
ACCGGGTGCTGTAGGCTTT130900.16551470204255764No Hit
TGAGGTAGTAAGTTGTATTGTT129890.1642376214538412No Hit
TATTGCACTTGTCCCGGCCTGTT128140.16202485805755032No Hit
ACCCCCCACTGCTAAATTTGACTG127580.16131677377073725No Hit
AACCGGGCGGAAACACCA127270.16092479854053712No Hit
TATTGCACTTGTCCCGGCCTGTAG120540.15241514273651563No Hit
TTCAAGTAATCCAGGATAGGC119200.15072079819307005No Hit
CACTAGATTGTGAGCTCCTGGA118260.14953222814020523No Hit
CAAAGTGCTGTTCGTGCAGGTAG117420.14847010170998562No Hit
ATCACATTGCCAGGGATTTCC117310.1483310137250759No Hit
TGAGGTAGTAGTTTGTGCTGTT114580.14487910282686214No Hit
TCCCACTGCTTCACTTGACTAG112720.14252725144566156No Hit
TGATGTAGTAGGTTGTATAGTT111420.14088348435127404No Hit
TGGCTCAGTTCAGCAGGAAC110630.139884579732377No Hit
AGCTCGGTCTGAGGCCCCTCAGT110190.13932822779273818No Hit
CACTAGATTGTGAGCTCCTGGAA109740.1387592314908348No Hit
ACTGGACTTGGAGTCAGAAGGCT105210.13303133538500755No Hit
TAGCAGCACATCATGGTTTACA103640.13104617050947803No Hit
CAAAGAATTCTCCTTTTGGGCTT102680.12983231173208418No Hit
TCCACCACGTTCCCGTGG102050.12903571690941945No Hit
AGCTACATTGTCTGCTGGGTTT101900.12884605147545167No Hit
ACCGGGTGCTGTAGGCTTA95790.12112034613183037No Hit
ACTGGACTTGGAGTCAGAAGGCA94470.11945129031291384No Hit
GTCAGGATGGCCGAGCGGTCTAAGG90440.11435561232031255No Hit
TGTAAACATCCCCGACTGGAAGC87870.1111060112183311No Hit
ATGCACCTGGGCAAGGATTCTGA87280.11035999384472445No Hit
TTCACAGTGGCTAAGTTCCG86770.10971513136923397No Hit
TGAGGTAGTAGATTGTATAGTTA86730.10966455392017588No Hit
TCCCACTGCTTCACTTGACTAGCCT85090.10759087850879473No Hit
TTCAAGTAATCCAGGATAGGCTT80840.10221702454637402No Hit
CAAAGAATTCTCCTTTTGGGC80800.10216644709731594No Hit
TATTGCACTTGTCCCGGCCTGTAAA79570.10061119053878007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)