FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170162
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG108671411.8505430983662No Hit
CCCCCCACTGCTAAATTTGACTG7788768.493590407672187No Hit
TTCAAGTAATCCAGGATAGGCT3110333.3917939508593196No Hit
CCCCCACTGCTAAATTTGACTG2371102.5856686065088055No Hit
AACATTCAACGCTGTCGGTGAGT1664771.8154204909357108No Hit
TAGCAGCACGTAAATATTGGCG1466851.5995900617677201No Hit
AACATTCAACGCTGTCGGTGAG1001131.091725533311189No Hit
AAGCTGCCAGTTGAAGAACTGT888980.9694267124179485No Hit
CATAAAGTAGAAAGCACTACT864500.9427314370236861No Hit
AACCGGGCGGAAACACCA785830.8569423310078927No Hit
CTCCCACTGCTTCACTTGACTAG741260.8083390457006102No Hit
ACTGGACTTGGAGTCAGAAGGC696750.7598011899898824No Hit
CCCATAAAGTAGAAAGCACTAC668590.7290928993402733No Hit
TCGTACGACTCTTAGCGG657810.7173373818259698No Hit
CAACGGAATCCCAAAAGCAGCTGT620150.6762694050552215No Hit
CGCCTGTCTGAGCGTCGCT614520.6701299279118514No Hit
CCCCCACTGCTAAATTTGACTGGCTT575490.6275679753531072No Hit
GCCTGTCTGAGCGTCGCT569990.6215702623356054No Hit
AGCAGCATTGTACAGGGCTATGA544860.5941661663120019No Hit
TAGCTTATCAGACTGATGTTGA491780.5362827832267303No Hit
CCCCCCACTGCTAAATTTGACTGGCT491440.5359120155129212No Hit
CACCCGGCTGTGTGCACATGTGC487190.5312774190903061No Hit
CCCATAAAGTAGAAAGCACT483300.5270354002470186No Hit
CTACGCCTGTCTGAGCGTCGCT471860.5145601571706149No Hit
CAACGGAATCCCAAAAGCAGCT421800.4599700637785897No Hit
CATTGCACTTGTCTCGGTCTGA419050.4569712072698388No Hit
TTCAAGTAATTCAGGATAGGTT409430.44648066195559033No Hit
TAGCTTATCAGACTGATGTTGAC374300.40817163317289273No Hit
CTTCTCACTACTGCACTTGACTAG348080.37957889947854795No Hit
CAACACCAGTCGATGGGCTGT345220.376460088709447No Hit
TATTGCACTTGTCCCGGCCTGTAA316610.34526107608567874No Hit
CACTAGATTGTGAGCTCCTGGA316220.34483578370807405No Hit
CCCCCCACTGCTAAATTTGACTGG312800.3411062967044639No Hit
CACTAGATTGTGAGCTCCTGGAA304440.3319897729178612No Hit
ACCGGGTGCTGTAGGCTTA287270.3132660033704966No Hit
TACGCCTGTCTGAGCGTCGCT285090.3108887280290141No Hit
CAAAGAATTCTCCTTTTGGGCT277800.3029390320476345No Hit
TGAGGTAGTAGGTTGTATAGTT275280.30019098899234276No Hit
TTCTCACTACTGCACTTGACTAG273260.29798819257500575No Hit
AGCTCGGTCTGAGGCCCCTCAGT271060.2955891073680051No Hit
ACCGGGTGCTGTAGGCTT260530.284106213172679No Hit
TATTGCACTTGTCCCGGCCTGT241310.26314693240970005No Hit
TATTGCACTTGTCCCGGCCTGTAT229280.2500282983005099No Hit
ATCCCGGACGAGCCCCCA207760.2265608830029393No Hit
TGAGGTAGTAGATTGTATAGTT205770.22439080138387957No Hit
CAAAGAATTCTCCTTTTGGGCTT203870.22231886415965169No Hit
TAGCTTATCAGACTGATGTTGACT201080.21927638791986442No Hit
CGCGACCTCAGATCAGACGT200670.2188292856767416No Hit
TGTAAACATCCTACACTCTCAGCT199880.21796779598877314No Hit
AACGGAATCCCAAAAGCAGCTG198180.21611395741972717No Hit
ACGCCTGTCTGAGCGTCGCT197170.21501255921105863No Hit
ACTGGACTTGGAGTCAGAAGGCT188430.2054816479796104No Hit
ACCGGGTGCTGTAGGCTTT187430.20439115470370098No Hit
TTCACAGTGGCTAAGTTCCG183890.20053080850698166No Hit
CTCCCACTGCTTCACTTGACTAGCCT179790.19605978607575308No Hit
TCCTGTCTGAGCGTCGCT176500.19247206319801113No Hit
TTCAAGTAATCCAGGATAGGCTT170820.18627806139084566No Hit
ACTGGACTTGGAGTCAGAAGG160840.17539493849726973No Hit
CCCCCACTGCTAAATTTGACTGGCT160110.17459887840585586No Hit
CGCGACCTCAGATCAGACGTGGC159040.1734320506006328No Hit
GGAATACCGGGTGCTGTAGGCTTT157750.17202531427470963No Hit
CCCATAAAGTAGAAAGCACTA155080.16911369722803152No Hit
TATTGCACTTGTCCCGGCCTGTA151180.16486077345198483No Hit
ACTGGACTTGGAGTCAGAAGGCA144990.15811062007410556No Hit
GACCCGCGGGCGCTCTCCAGTCCT141750.15457742186015905No Hit
ACCACAGGGTAGAACCACGGAC135000.14721659224777053No Hit
AACATTCAACGCTGTCGGTGAGTT134920.14712935278569778No Hit
ACCCCCCACTGCTAAATTTGACTG134570.1467476801391295No Hit
TTCAAGTAATCCAGGATAGGC130580.14239661196825093No Hit
CAACGGAATCCCAAAAGCAGCTGA128400.14001933662676844No Hit
TTCACAGTGGCTAAGTTCCGC124040.1352647859438034No Hit
TCGTTTCCCGGGCGGCGCACCA122300.13336732764372103No Hit
CCCCCACTGCTAAATTTGACTGGCTA120890.13182973212468876No Hit
AGCAGCATTGTACAGGGCTATGT116630.12718423076931465No Hit
GGAATACCGGGTGCTGTAGGCTT115340.1257774944433915No Hit
TCCCACTGCTTCACTTGACTAG114060.12438166305022748No Hit
ATCCCACCGCTGCCACCA111190.12125194734836746No Hit
CGCGACCTCAGATCAGACGTGGCG108400.11820947110858021No Hit
CCCCCCACTGCTAAATTTGACTGGC104730.11420736078599267No Hit
TCGTTTCCCGGCCAATGCACCA100820.10994353207718686No Hit
TGTAAACATCCTTGACTGGAAGCT99390.10838412669263639No Hit
CAAAGTGCTGTTCGTGCAGGT96130.10482911861317172No Hit
CAGTGCAATAGTATTGTCAAAGC93820.10231007914582098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)