FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12438754
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG231413318.60421871837002No Hit
CAACGGAATCCCAAAAGCAGCTG8941377.188316450345429No Hit
CCCCCACTGCTAAATTTGACTG5938584.774256328246382No Hit
TAGCAGCACGTAAATATTGGCG4397073.534976252444578No Hit
AAGCTGCCAGTTGAAGAACTGT3577502.876091930108112No Hit
TAGCTTATCAGACTGATGTTGAC3411572.7426943245280033No Hit
TTCAAGTAATCCAGGATAGGCT3312042.6626782714731716No Hit
AACATTCAACGCTGTCGGTGAGT2634542.1180095691256535No Hit
TAGCTTATCAGACTGATGTTGACT2489482.0013901713949807No Hit
AACATTCAACGCTGTCGGTGAG1666481.3397483381374051No Hit
TGTAAACATCCTTGACTGGAAGCT1628931.3095604270331256No Hit
TATTGCACTTGTCCCGGCCTGTAA1405281.1297594598301406No Hit
TATTGCACTTGTCCCGGCCTGTAT1291001.037885305875492No Hit
TAGCTTATCAGACTGATGTTGA1217510.9788038255278624No Hit
AGCAGCATTGTACAGGGCTATGA953700.7667166663156133No Hit
TGAGGTAGTAGGTTGTATAGTT875190.7035994119668256No Hit
TGTAAACATCCCCGACTGGAAGCT679960.5466463923958943No Hit
TATTGCACTTGTCCCGGCCTGT663440.5333653193880994No Hit
CATAAAGTAGAAAGCACTACT650630.5230668602337502No Hit
CAACGGAATCCCAAAAGCAGCTGT596430.4794933640459486No Hit
ACTGGACTTGGAGTCAGAAGGC585440.47065807395178005No Hit
TATTGCACTTGTCCCGGCCTGTA557030.44781816571016675No Hit
TTCAAGTAATTCAGGATAGGTT553690.4451330093030218No Hit
TGTAAACATCCTACACTCTCAGCT530660.4266182931184265No Hit
TGAGGTAGTAGATTGTATAGTT510300.410250094181459No Hit
CGCCTGTCTGAGCGTCGCT485180.3900551453947879No Hit
TAGCTTATCAGACTGATGTTGACA454740.3655832408937422No Hit
TGTCAGTTTGTCAAATACCCCAA437570.3517796075073114No Hit
ACCCCCCACTGCTAAATTTGACTG414910.3335623487690166No Hit
CCCATAAAGTAGAAAGCACTAC395980.31834378266504826No Hit
GCCTGTCTGAGCGTCGCT367350.2953270078337428No Hit
CATTGCACTTGTCTCGGTCTGA360920.29015767978046675No Hit
CCCCCCACTGCTAAATTTGACTGG354670.2851330607551206No Hit
CAAAGAATTCTCCTTTTGGGCT348790.280405899176075No Hit
CCCCCACTGCTAAATTTGACTGGCTT336020.2701395975834879No Hit
CTTCTCACTACTGCACTTGACTAG328570.2641502517052753No Hit
AACCGGGCGGAAACACCA318590.25612694004560266No Hit
CAAAGAATTCTCCTTTTGGGCTT306410.24633496248900816No Hit
CAACGGAATCCCAAAAGCAGCT287210.23089933284314493No Hit
TAGCAGCACGTAAATATTGGC284910.22905027304181755No Hit
TGTCAGTTTGTCAAATACCCCA275170.22121990675271816No Hit
CACCCGGCTGTGTGCACATGTGC268840.21613097260384762No Hit
AACATTCAACGCTGTCGGTGAGTT265150.21316443753128328No Hit
TTCTCACTACTGCACTTGACTAG240140.1930579220394583No Hit
CCCCCCACTGCTAAATTTGACTGGCT232060.18656209456349085No Hit
TTCAAGTAATCCAGGATAGGCTT228240.1834910474151993No Hit
TAGCAGCACGTAAATATTGGCGT227400.1828157386181928No Hit
CAACACCAGTCGATGGGCTGT223470.17965625817505515No Hit
CCCATAAAGTAGAAAGCACT214970.17282277630058443No Hit
CTACGCCTGTCTGAGCGTCGCT214370.17234041287415122No Hit
TACGCCTGTCTGAGCGTCGCT214150.17216354628445904No Hit
TGAGGTAGTAGTTTGTACAGTT210860.16951858682951684No Hit
TGTAAACATCCTACACTCAGCT203110.16328805923808767No Hit
CACTAGATTGTGAGCTCCTGGAA201650.16211430823376682No Hit
AACGGAATCCCAAAAGCAGCTG198410.1595095457310274No Hit
ATCACATTGCCAGGGATTTCCA195800.15741126482604287No Hit
CCCCCCACTGCTAAATTTGACTGT188280.15136564321474644No Hit
CGCCCCACTGCTAAATTTGACTG185460.14909853511051027No Hit
AGCAGCATTGTACAGGGCTATGT184830.14859205351275537No Hit
CTCCCACTGCTTCACTTGACTAG183620.14761928726944837No Hit
ACTGGACTTGGAGTCAGAAGGCA183510.14753085397460228No Hit
CACTAGATTGTGAGCTCCTGGA182430.14666259980702248No Hit
TGTAAACATCCCCGACTGGAAGC178320.14335841033595487No Hit
GCCCCCACTGCTAAATTTGACTG163400.13136363979864865No Hit
TCCTGTCTGAGCGTCGCT163120.13113853686631316No Hit
TTATAAAGCAATGAGACTGATT162060.13028636147961445No Hit
CAGTGCAATAGTATTGTCAAAGC156470.12579234222334487No Hit
TGAGGTAGTAGTTTGTGCTGTT152950.12296247678826996No Hit
TATTGCACTTGTCCCGGCCTGTAG152910.12293031922650773No Hit
TATTGCACTTGTCCCGGCCTGTT146640.11788962142028053No Hit
TATTGCACTTGTCCCGGCCTGTAAT146360.11766451848794501No Hit
GCGTTCGCGCTTTCCCCTG144610.1162576251608481No Hit
CAAAGTGCTGTTCGTGCAGGTAG141320.11361266570590592No Hit
ATCACATTGCCAGGGATTTCC139860.11243891470158505No Hit
TTCACAGTGGCTAAGTTCCGC136980.11012357025470558No Hit
TCGTTTCCCGGCCAATGCACCA135350.1088131496128953No Hit
TTCAAGTAATCCAGGATAGGC134650.10825039228205655No Hit
GGAATACCGGGTGCTGTAGGCTTT134250.10792881666443441No Hit
TGTAAACATCCTTGACTGGAAGC131390.10562955099843602No Hit
CTCCCACTGCTTCACTTGACTAGCCT129150.10382872753975199No Hit
TTTAAACATCCTTGACTGGAAGCT129100.1037885305875492No Hit
TCAGTGCACTACAGAACTTTGT128540.10333832472287818No Hit
TGTCAGTTTGTCAAATACCCC127370.1023977160413334No Hit
ACCACAGGGTAGAACCACGGAC127190.10225300701340342No Hit
ACTGGACTTGGAGTCAGAAGGCT124630.10019492306062167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)