FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357315
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG147296214.221465698397703No Hit
CAACGGAATCCCAAAAGCAGCTG8925238.617320222470786No Hit
CCCCCACTGCTAAATTTGACTG4190784.046203094141677No Hit
AACATTCAACGCTGTCGGTGAGT3570273.447099948200861No Hit
TAGCAGCACGTAAATATTGGCG3079512.973270582192393No Hit
AAGCTGCCAGTTGAAGAACTGT3025262.920892142413357No Hit
TTCAAGTAATCCAGGATAGGCT3009142.9053282631647295No Hit
AACATTCAACGCTGTCGGTGAG2276982.1984269089044797No Hit
TATTGCACTTGTCCCGGCCTGTAT1867141.8027258995212563No Hit
TAGCTTATCAGACTGATGTTGACT1804871.7426041401656704No Hit
TATTGCACTTGTCCCGGCCTGTAA1728301.668675713734689No Hit
TAGCTTATCAGACTGATGTTGAC1714021.6548883566831751No Hit
TAGCTTATCAGACTGATGTTGA1054671.0182851443641523No Hit
TATTGCACTTGTCCCGGCCTGT1042591.0066218899396224No Hit
TGTAAACATCCTTGACTGGAAGCT894940.8640656386331786No Hit
TGAGGTAGTAGGTTGTATAGTT857810.8282165792968544No Hit
TATTGCACTTGTCCCGGCCTGTA781960.7549833137256132No Hit
CATAAAGTAGAAAGCACTACT777600.7507737285194087No Hit
AGCAGCATTGTACAGGGCTATGA685320.661677278329374No Hit
TGAGGTAGTAGATTGTATAGTT585570.5653685342195347No Hit
TGTAAACATCCCCGACTGGAAGCT572470.5527204685770395No Hit
CAACGGAATCCCAAAAGCAGCTGT558650.5393772420748041No Hit
CCCATAAAGTAGAAAGCACTAC528960.510711511622462No Hit
TTCAAGTAATTCAGGATAGGTT508620.4910732173348016No Hit
ACTGGACTTGGAGTCAGAAGGC496350.47922651768339575No Hit
TCGTACGACTCTTAGCGG439630.42446328995497384No Hit
CACCCGGCTGTGTGCACATGTGC420190.40569394674198866No Hit
CATTGCACTTGTCTCGGTCTGA382080.36889869623546256No Hit
CCCATAAAGTAGAAAGCACT361140.34868110123135193No Hit
TTAATGCTAATCGTGATAGGGGTT356690.3443846209176799No Hit
CAACGGAATCCCAAAAGCAGCT317360.3064114589543719No Hit
TGTAAACATCCTACACTCTCAGCT316390.30547492279611077No Hit
CTCCCACTGCTTCACTTGACTAG297250.2869952299413506No Hit
CCCCCACTGCTAAATTTGACTGGCTT297210.2869566098935873No Hit
CAAAGAATTCTCCTTTTGGGCT278270.2686700172776439No Hit
ACCCCCCACTGCTAAATTTGACTG276170.2666424647700683No Hit
TTCTCACTACTGCACTTGACTAG275330.2658314437670381No Hit
CTTCTCACTACTGCACTTGACTAG267310.2580881241904876No Hit
AACATTCAACGCTGTCGGTGAGTT262800.25373371380517057No Hit
CACTAGATTGTGAGCTCCTGGA260880.25187995151253006No Hit
CACTAGATTGTGAGCTCCTGGAA253970.24520833826141233No Hit
TGAGGTAGTAGTTTGTACAGTT247880.23932843598944323No Hit
TGAGAACTGAATTCCATAGGCTGT243930.23551470627281296No Hit
CAACACCAGTCGATGGGCTGT243810.23539884612952294No Hit
TAGCTTATCAGACTGATGTTGACA242590.23422093467274094No Hit
ATCCCGGACGAGCCCCCA241160.2328402679652014No Hit
TATTGCACTTGTCCCGGCCTGTT227070.21923635614056347No Hit
CCCCCCACTGCTAAATTTGACTGG225410.21763362415838466No Hit
TATTGCACTTGTCCCGGCCTGTAG222450.21477574062389723No Hit
AGCTCGGTCTGAGGCCCCTCAGT216460.20899238847133642No Hit
TGTCAGTTTGTCAAATACCCCAA215920.20847101782653132No Hit
CCCCCCACTGCTAAATTTGACTGGCT215130.20770827188320526No Hit
CGCCTGTCTGAGCGTCGCT211920.2046090130501969No Hit
AACGGAATCCCAAAAGCAGCTG201480.1945291805839641No Hit
CAAAGAATTCTCCTTTTGGGCTT198880.19201887747934673No Hit
TATTGCACTTGTCCCGGCCTGTTT191770.1851541639894123No Hit
AACCGGGCGGAAACACCA190020.18346453689976602No Hit
ACCGGGTGCTGTAGGCTTA185340.17894599131145475No Hit
TTCAAGTAATCCAGGATAGGCTT181960.17568259727545218No Hit
CCCCCCACTGCTAAATTTGACTGT177740.17160818223641938No Hit
TGAGAACTGAATTCCATGGGTT176420.17033372066022903No Hit
TAGCAGCACGTAAATATTGGC175850.16978338497960135No Hit
TCGTTTCCCGGGCGGCGCACCA174800.1687696087258136No Hit
TGAGGTAGTAGTTTGTGCTGTT162630.15701945919381616No Hit
TGTCAGTTTGTCAAATACCCCA161280.15571603258180328No Hit
ACCGGGTGCTGTAGGCTT160040.15451881110113963No Hit
TGTAAACATCCCCGACTGGAAGC159600.15409399057574286No Hit
ACTGGACTTGGAGTCAGAAGGCA153860.14855201372170299No Hit
TCGTTTCCCGGCCAATGCACCA151580.14635067099919236No Hit
TATTGCACTTGTCCCGGCCTGTAAT151010.1458003353185647No Hit
ACTGGACTTGGAGTCAGAAGGCT149790.14462242386178273No Hit
AGCAGCATTGTACAGGGCTATGT145260.140248703452584No Hit
CTCCCACTGCTTCACTTGACTAGCCT136700.13198401323122835No Hit
GCCTGTCTGAGCGTCGCT135130.1304681763565171No Hit
ACCGGGTGCTGTAGGCTTT134590.12994680571171197No Hit
TTCACAGTGGCTAAGTTCCG134230.12959922528184187No Hit
TTCAAGTAATCCAGGATAGGC134230.12959922528184187No Hit
CCCATAAAGTAGAAAGCACTA129840.12536067503981485No Hit
CTACGCCTGTCTGAGCGTCGCT127580.1231786423411859No Hit
GACCCGCGGGCGCTCTCCAGTCCT126270.12191383577693639No Hit
TCAGTGCACTACAGAACTTTGT124800.12049454902163349No Hit
CCCCCCACTGCTAAATTTGACTGA119030.11492360713177112No Hit
CAAAGTGCTGTTCGTGCAGGTAG118080.11400638099739169No Hit
TATTGCACTTGTCCCGGCCTGTAAA117480.11342708028094155No Hit
TTATAAAGCAATGAGACTGATT116780.11275122944508302No Hit
TTCACAGTGGCTAAGTTCCGC114660.11070436691362577No Hit
ACCACAGGGTAGAACCACGGAC113640.1097195556956605No Hit
ATCACATTGCCAGGGATTTCC110410.10660098683877048No Hit
TTCACAGTGGCTAAGTTCTGC107720.10400378862668559No Hit
ACTGGACTTGGAGTCAGAAGG104960.10133900533101485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)