FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872932
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8253299.301649105391544No Hit
CAACGGAATCCCAAAAGCAGCTG7279558.204221558330437No Hit
AAGCTGCCAGTTGAAGAACTGT3301683.7210698785925556No Hit
TTCAAGTAATCCAGGATAGGCT2832573.192372036661613No Hit
CCCCCACTGCTAAATTTGACTG2495312.812272200440621No Hit
TAGCAGCACGTAAATATTGGCG2481992.797260251740913No Hit
AACATTCAACGCTGTCGGTGAGT2341412.638823333707505No Hit
TAGCTTATCAGACTGATGTTGAC1769911.994729588821373No Hit
AACATTCAACGCTGTCGGTGAG1529091.7233198676604307No Hit
TAGCTTATCAGACTGATGTTGACT1233271.389923871838531No Hit
TGAGGTAGTAGGTTGTATAGTT989961.1157078629702109No Hit
TATTGCACTTGTCCCGGCCTGTAA971911.0953650946496605No Hit
GCCTGTCTGAGCGTCGCT903591.0183668712889944No Hit
TATTGCACTTGTCCCGGCCTGTAT890541.0036592188467126No Hit
TGTAAACATCCTTGACTGGAAGCT853360.9617564971759054No Hit
TAGCTTATCAGACTGATGTTGA840750.9475447349309113No Hit
TATTGCACTTGTCCCGGCCTGT828280.9334907559305087No Hit
AGCAGCATTGTACAGGGCTATGA657480.7409951975288439No Hit
CATAAAGTAGAAAGCACTACT615650.6938518180912465No Hit
CGCCTGTCTGAGCGTCGCT575760.6488948636144174No Hit
TTCAAGTAATTCAGGATAGGTT569410.6417382664490159No Hit
TGAGGTAGTAGATTGTATAGTT550890.6208657972359081No Hit
AACCGGGCGGAAACACCA521740.5880130716656006No Hit
CAACGGAATCCCAAAAGCAGCTGT482910.5442507617549645No Hit
TATTGCACTTGTCCCGGCCTGTA473120.5332172048653139No Hit
TGTAAACATCCCCGACTGGAAGCT413600.4661367854504013No Hit
CCCATAAAGTAGAAAGCACTAC404460.45583579362492577No Hit
TTAATGCTAATCGTGATAGGGGTT382910.43154844418958693No Hit
TGTAAACATCCTACACTCTCAGCT366500.4130539938771085No Hit
ACTGGACTTGGAGTCAGAAGGC363400.4095602220325818No Hit
CATTGCACTTGTCTCGGTCTGA325340.3666657199671991No Hit
TCGTACGACTCTTAGCGG321000.36177443938486176No Hit
CACCCGGCTGTGTGCACATGTGC271840.30636998006972216No Hit
CTACGCCTGTCTGAGCGTCGCT269340.3035524221305877No Hit
TAGCTTATCAGACTGATGTTGACA241620.2723113397014651No Hit
CCCATAAAGTAGAAAGCACT227650.25656682593758184No Hit
TGAGGTAGTAGTTTGTACAGTT226040.25475231862477926No Hit
ACCCCCCACTGCTAAATTTGACTG220490.2484973399999008No Hit
TACGCCTGTCTGAGCGTCGCT207430.23377841732586252No Hit
AACATTCAACGCTGTCGGTGAGTT202830.22859411071785518No Hit
CCCCCACTGCTAAATTTGACTGGCTT199340.22466079983482345No Hit
ACCGGGTGCTGTAGGCTT194100.2187551983943977No Hit
CTCCCACTGCTTCACTTGACTAG192970.21748166220590895No Hit
CAACACCAGTCGATGGGCTGT186560.21025744364996826No Hit
TTCTCACTACTGCACTTGACTAG185960.20958122974457596No Hit
CAACGGAATCCCAAAAGCAGCT180460.2033826022784802No Hit
TGAGAACTGAATTCCATAGGCTGT174970.197195245044141No Hit
TGTCAGTTTGTCAAATACCCCAA174910.19712762365360176No Hit
TGATGTAGTAGGTTGTATAGTT171150.19289001651314358No Hit
TAGCAGCACGTAAATATTGGC169870.19144742684830673No Hit
ACGCCTGTCTGAGCGTCGCT168530.18993721579293069No Hit
TGTCAGTTTGTCAAATACCCCA167610.1889003544713292No Hit
TGAGGTAGTAGTTTGTGCTGTT166930.18813397871188464No Hit
CAAAGAATTCTCCTTTTGGGCT165450.18646598441191706No Hit
CCCCCCACTGCTAAATTTGACTGG161040.1814958122072839No Hit
TTCAAGTAATCCAGGATAGGCTT160650.18105627316877893No Hit
AACGGAATCCCAAAAGCAGCTG158830.17900509098908907No Hit
TCGTTTCCCGGCCAATGCACCA155160.1748689159344397No Hit
AGCAGCATTGTACAGGGCTATGT149510.16850123499199587No Hit
CTTCTCACTACTGCACTTGACTAG148770.1676672378420121No Hit
CCCCCCACTGCTAAATTTGACTGGCT144800.16319295583466661No Hit
TATTGCACTTGTCCCGGCCTGTT143290.16149115083942941No Hit
CAAAGTGCTGTTCGTGCAGGTAG142120.1601725337239145No Hit
TGTAAACATCCTACACTCAGCT142010.16004856117459257No Hit
GGAATACCGGGTGCTGTAGGCTTT141650.15964283283135722No Hit
ACCGGGTGCTGTAGGCTTT140830.15871867382732113No Hit
TTCAAGTAATCCAGGATAGGC140430.1582678645570596No Hit
AGCTCGGTCTGAGGCCCCTCAGT138220.15577714333886475No Hit
TGAGGTAGTAGGTTGTGTGGTT131590.14830497968428025No Hit
TTCACAGTGGCTAAGTTCCGC125950.14194856897359295No Hit
GGGCTACGCCTGTCTGAGCGTCGCT124330.14012279142903383No Hit
ATCACATTGCCAGGGATTTCC120640.13596407591087142No Hit
GCGTTCGCGCTTTCCCCTG118080.13307889658119773No Hit
CAAAGAATTCTCCTTTTGGGCTT117130.13200822456432665No Hit
TTCACAGTGGCTAAGTTCCG114220.12872858712317417No Hit
ATCACATTGCCAGGGATTTCCA112130.12637310868605778No Hit
CCACCCACTGCTAAATTTGACTG111910.12612516358741394No Hit
ACCGGGTGCTGTAGGCTTA111900.1261138933556574No Hit
CCCACCACTGCTAAATTTGACTG111650.12583213756174397No Hit
ACCACAGGGTAGAACCACGGAC110530.12456987160501173No Hit
GACCCGCGGGCGCTCTCCAGTCCT110300.12431065627461137No Hit
GGAATACCGGGTGCTGTAGGCTT110270.12427684557934175No Hit
TGTAAACATCCCCGACTGGAAGC110220.12422049442055907No Hit
TTATAAAGCAATGAGACTGATT108880.12271028336518301No Hit
TAGCAGCACGTAAATATTGGCGT108690.1224961489618088No Hit
TATTGCACTTGTCCCGGCCTGTAG107090.12069291188076275No Hit
TTCACAGTGGCTAAGTTCTGC104330.11758232791595834No Hit
AGCAGCATTGTACAGGGCTATG100560.1133334505437436No Hit
CACCCCACTGCTAAATTTGACTG99810.11248818316200326No Hit
AGAGGTAGTAGGTTGCATAGTT98110.11057224376339184No Hit
ATCCCGGACGAGCCCCCA96350.10858868297424121No Hit
TGATGTAGTAGATTGTATAGTT95420.1075405514208832No Hit
CACTAGATTGTGAGCTCCTGGA93210.10504983020268835No Hit
TCGTTTCCCGGGCGGCGCACCA90230.1016913011392401No Hit
TGAGGTAGTAGGTTGTATAGT88980.10028252216967289No Hit
CACTAGATTGTGAGCTCCTGGAA88800.10007965799805521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)