FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6272375
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG105840116.874007054744016No Hit
CCCCCCACTGCTAAATTTGACTG92972414.822519380617392No Hit
CCCCCACTGCTAAATTTGACTG3098784.940361505809203No Hit
TTCAAGTAATCCAGGATAGGCT1891763.016018653221467No Hit
TAGCAGCACGTAAATATTGGCG1269052.0232368122122804No Hit
AACATTCAACGCTGTCGGTGAG1152861.8379959744116066No Hit
TAGCTTATCAGACTGATGTTGAC1110421.7703342035512863No Hit
TGAGGTAGTAGATTGTATAGTT858631.3689073118236714No Hit
TCGTACGACTCTTAGCGG786551.2539907132465773No Hit
AAGCTGCCAGTTGAAGAACTGT783071.2484425755794257No Hit
TATTGCACTTGTCCCGGCCTGTAA744471.1869028876621694No Hit
TGAGAACTGAATTCCATAGGCTGT737831.176316785906455No Hit
TAGCTTATCAGACTGATGTTGACT714771.1395524023994101No Hit
TTAATGCTAATCGTGATAGGGGTT696901.1110623966201No Hit
TATTGCACTTGTCCCGGCCTGTAT644491.0275055302018772No Hit
TGAGGTAGTAGGTTGTATAGTT630841.005743438490205No Hit
TGAGAACTGAATTCCATGGGTT606710.9672731620797544No Hit
TAGCTTATCAGACTGATGTTGA570650.9097829769425457No Hit
TATTGCACTTGTCCCGGCCTGT505500.8059148249267623No Hit
CAACGGAATCCCAAAAGCAGCTGT465810.7426373582574384No Hit
TGTAAACATCCTTGACTGGAAGCT419180.6682955021024731No Hit
TATTGCACTTGTCCCGGCCTGTA382590.6099603419757269No Hit
CAACGGAATCCCAAAAGCAGCT359250.572749556587417No Hit
CCCCCACTGCTAAATTTGACTGGCTT342940.5467466470037267No Hit
AACATTCAACGCTGTCGGTGAGT337810.5385679268219774No Hit
TTCAAGTAATTCAGGATAGGTT324430.5172362940672393No Hit
AGCAGCATTGTACAGGGCTATGA323020.514988341736583No Hit
CACCCGGCTGTGTGCACATGTGC300050.4783674445485163No Hit
TGAGGTAGTAGTTTGTACAGTT257590.41067378783953445No Hit
ACCCCCCACTGCTAAATTTGACTG248840.396723729050001No Hit
TGTAAACATCCCCGACTGGAAGCT228200.3638175332310329No Hit
CAAAGAATTCTCCTTTTGGGCT226720.3614579804300604No Hit
AACGGAATCCCAAAAGCAGCTG216280.3448135674286056No Hit
CATTGCACTTGTCTCGGTCTGA214240.34156121086510294No Hit
TCGTTTCCCGGCCAATGCACCA206570.3293329879033062No Hit
CATAAAGTAGAAAGCACTACT204110.3254110285179059No Hit
TTCTCACTACTGCACTTGACTAG201680.32153689790549833No Hit
TAGCTTATCAGACTGATGTTGACA199230.31763088144442897No Hit
CCCATAAAGTAGAAAGCACTAC194790.3105522230415114No Hit
TTAATGCTAATCGTGATAGGGGTTT162630.2592797783933518No Hit
ACTGGACTTGGAGTCAGAAGGC157350.250861914346639No Hit
CTTCTCACTACTGCACTTGACTAG156810.2500009964327707No Hit
CACTAGATTGTGAGCTCCTGGAA145130.23137966081428485No Hit
TAGCAGCACGTAAATATTGGC144550.23045497120309294No Hit
CCCCCCACTGCTAAATTTGACTGG135720.21637736901891227No Hit
CACTAGATTGTGAGCTCCTGGA128920.20553618047390343No Hit
AATCCTTGGAACCTAGGTGTGAGT128410.20472309133302774No Hit
CTCCCACTGCTTCACTTGACTAG128190.20437234699774803No Hit
CCCATAAAGTAGAAAGCACT125390.19990832818509735No Hit
CCCATAAAGTAGAAAGCACTA122750.19569939616174095No Hit
TGTAAACATCCTACACTCTCAGCT122580.19542836644811573No Hit
CCCCCCACTGCTAAATTTGACTGGCT106040.16905876960481475No Hit
CTCCCACTGCTTCACTTGACTAGCCT103310.16470635126248032No Hit
ATGCACCTGGGCAAGGATTCTGA100030.15947707208194664No Hit
TCGTTTCCCGGCCAACGCACCA95720.1526056716953307No Hit
TGAGAACTGAATTCCATGGGTTG94330.15038960521333625No Hit
CAAAGAATTCTCCTTTTGGGC94020.1498953745590785No Hit
TTCACAGTGGCTAAGTTCCGC93930.14975188824010044No Hit
TATTGCACTTGTCCCGGCCTGTT93270.14869965523426135No Hit
GGAATACCGGGTGCTGTAGGCTTT92650.14771119392574583No Hit
AATGCACCCGGGCAAGGATTCT92560.14756770760676777No Hit
AGCTCGGTCTGAGGCCCCTCAGT91620.1460690727196636No Hit
TATTGCACTTGTCCCGGCCTGTAG91490.14586181470336196No Hit
CAACACCAGTCGATGGGCTGT90300.14396460670798542No Hit
CAAAGAATTCTCCTTTTGGGCTT87850.14005859024691603No Hit
GCCTGTCTGAGCGTCGCT85820.1368221766077443No Hit
AGCTACATTGTCTGCTGGGTTT82660.13178421251918135No Hit
CACCCGTAGAACCGACCTTGCG81600.13009426254010642No Hit
TTAATGCTAATCGTGATAGGGGT81380.12974351820482674No Hit
ATCACATTGCCAGGGATTTCC80780.128786942744973No Hit
TGTCAGTTTGTCAAATACCCCA80100.1277028238904721No Hit
CAAAGTGCTGTTCGTGCAGGTAG78400.12499252675421989No Hit
TGAGGTAGTAGATTGTATAGT75720.12071982303353992No Hit
CCCCCCACTGCTAAATTTGACTGT74100.11813706929193488No Hit
GGAATACCGGGTGCTGTAGGCTT71780.11443831084716714No Hit
TGATGTAGTAGATTGTATAGTT70910.11305127643037925No Hit
TATTGCACTTGTCCCGGCCTGTAAA69050.1100858925048327No Hit
TAGCAGCACATCATGGTTTACA68910.10986269156420016No Hit
TTCACAGTGGCTAAGTTCTGC68570.10932063213694972No Hit
TTCAAGTAATCCAGGATAGGC67170.10708862273062436No Hit
TGAGGTAGTAGTTTGTGCTGTT67160.10707267980629347No Hit
TATTGCACTTGTCCCGGCCTGTAAT67080.1069451364116463No Hit
TGAGGTAGTAAGTTGTATTGTT64130.10224197373403217No Hit
ACCGGGTGCTGTAGGCTT63400.1010781402578768No Hit
ACTGGACTTGGAGTCAGAAGGCA63270.10087088224157517No Hit
TTCACAGTGGCTAAGTTCTG63130.10064768130094263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)