FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9567599
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8877339.278534771367404No Hit
CAACGGAATCCCAAAAGCAGCTG7807478.160323190802625No Hit
TTCAAGTAATCCAGGATAGGCT3587893.7500421997201183No Hit
TAGCAGCACGTAAATATTGGCG3391153.544410671893753No Hit
AACATTCAACGCTGTCGGTGAGT3383293.536195444646039No Hit
CCCCCACTGCTAAATTTGACTG3069173.207879009143255No Hit
AAGCTGCCAGTTGAAGAACTGT2802922.9295960250842454No Hit
AACATTCAACGCTGTCGGTGAG2096922.191688844818852No Hit
TAGCTTATCAGACTGATGTTGAC1831241.9140016215144469No Hit
TAGCTTATCAGACTGATGTTGACT1588841.660646521661286No Hit
TGAGGTAGTAGGTTGTATAGTT1344561.4053264565122348No Hit
TAGCTTATCAGACTGATGTTGA1300001.3587525982223962No Hit
TATTGCACTTGTCCCGGCCTGTAA1025531.0718781169653955No Hit
TGTAAACATCCTTGACTGGAAGCT977931.0221268679843292No Hit
CATAAAGTAGAAAGCACTACT953880.9969899449172148No Hit
TATTGCACTTGTCCCGGCCTGTAT911860.9530708801654417No Hit
TATTGCACTTGTCCCGGCCTGT795400.8313475512508415No Hit
TGAGGTAGTAGATTGTATAGTT716200.7485681621899078No Hit
AGCAGCATTGTACAGGGCTATGA714400.746686812438523No Hit
TTCAAGTAATTCAGGATAGGTT671290.7016284858928556No Hit
TATTGCACTTGTCCCGGCCTGTA545450.5701012343849278No Hit
CGCCTGTCTGAGCGTCGCT541830.5663176309960315No Hit
CCCATAAAGTAGAAAGCACTAC516960.5403236485977307No Hit
TGTAAACATCCCCGACTGGAAGCT487760.5098039748530431No Hit
ACTGGACTTGGAGTCAGAAGGC463570.48452072458304324No Hit
CAACGGAATCCCAAAAGCAGCTGT454510.47505126416773946No Hit
TGTCAGTTTGTCAAATACCCCAA371260.38803883816618984No Hit
GCCTGTCTGAGCGTCGCT360990.3773046926402329No Hit
CATTGCACTTGTCTCGGTCTGA358420.3746185432729779No Hit
TGAGGTAGTAGTTTGTACAGTT338510.3538087246340487No Hit
CCCATAAAGTAGAAAGCACT329410.34429745644649196No Hit
CAACACCAGTCGATGGGCTGT329150.34402570592684745No Hit
CCCCCACTGCTAAATTTGACTGGCTT320400.33488025574650443No Hit
AACATTCAACGCTGTCGGTGAGTT286950.2999185061999359No Hit
TTCTCACTACTGCACTTGACTAG269680.28186800052970445No Hit
CACCCGGCTGTGTGCACATGTGC266530.27857563846478095No Hit
TGTAAACATCCTACACTCTCAGCT258390.2700677568112961No Hit
CTACGCCTGTCTGAGCGTCGCT250430.26174801013294974No Hit
TAGCTTATCAGACTGATGTTGACA244400.2554454884658105No Hit
TACGCCTGTCTGAGCGTCGCT236570.24726161704728636No Hit
TTCACAGTGGCTAAGTTCCG235660.24631049022853072No Hit
TGTCAGTTTGTCAAATACCCCA219970.2299113915622927No Hit
TAGCAGCACGTAAATATTGGC218690.2285735428501968No Hit
TGAGGTAGTAGTTTGTGCTGTT209510.21897865911813405No Hit
CAAAGAATTCTCCTTTTGGGCT201760.2108784032441159No Hit
CTTCTCACTACTGCACTTGACTAG199620.20864168742858058No Hit
ACCGGGTGCTGTAGGCTT198950.20794140724334284No Hit
AACCGGGCGGAAACACCA196900.20579875891537677No Hit
AACGGAATCCCAAAAGCAGCTG196450.20532842147753058No Hit
TCGTACGACTCTTAGCGG194910.20371882224579022No Hit
ACCCCCCACTGCTAAATTTGACTG193770.2025273007365798No Hit
CAACGGAATCCCAAAAGCAGCT189170.19771940692748513No Hit
CTCCCACTGCTTCACTTGACTAG179030.18712113666135044No Hit
TTCAAGTAATCCAGGATAGGCTT175900.18384967848255346No Hit
CCCCCCACTGCTAAATTTGACTGGCT174920.18282538806235504No Hit
TATTGCACTTGTCCCGGCCTGTT162360.16969774757491404No Hit
TTATAAAGCAATGAGACTGATT161790.16910198682030883No Hit
TTCAAGTAATCCAGGATAGGC154130.16109579843385993No Hit
TTCACAGTGGCTAAGTTCCGC152250.15913083313796908No Hit
CCCCCCACTGCTAAATTTGACTGG151300.15813789854696042No Hit
ACGCCTGTCTGAGCGTCGCT147160.15381079411877524No Hit
AGCAGCATTGTACAGGGCTATGT146210.15281785952776658No Hit
TGTAAACATCCCCGACTGGAAGC145870.1524624934636161No Hit
CAAAGAATTCTCCTTTTGGGCTT144050.15056023982610475No Hit
TAGCAGCACGTAAATATTGGCGT139800.14611816402422384No Hit
ACCGGGTGCTGTAGGCTTT136720.14289896556074308No Hit
TGAGGTAGTAGGTTGTGTGGTT131600.1375475707123595No Hit
ACTGGACTTGGAGTCAGAAGGCA131460.13740124350947402No Hit
ACTGGACTTGGAGTCAGAAGGCT129150.13498684466186345No Hit
ACCGGGTGCTGTAGGCTTA128640.13445379556563775No Hit
TTCACAGTGGCTAAGTTCTGC127930.13371170760814705No Hit
TCGTTTCCCGGCCAATGCACCA124760.13039844165709705No Hit
TTCACAGTGGCTAAGTTCTG122990.12854844773490193No Hit
TGAGGTAGTAGGTTGTATAGT121240.12671935769883333No Hit
AGCAGCATTGTACAGGGCTATG120630.12608178917197513No Hit
ACCACAGGGTAGAACCACGGAC120550.12599817362746912No Hit
CACTAGATTGTGAGCTCCTGGA119640.12504704680871345No Hit
ACTGGACTTGGAGTCAGAAGG119080.12446173799717149No Hit
CAAAGTGCTGTTCGTGCAGGTAG118630.12399140055932528No Hit
CAAAGTGCTGTTCGTGCAGGT117970.12330157231715085No Hit
ATCACATTGCCAGGGATTTCC112920.11802334107020998No Hit
AGAGGTAGTAGGTTGCATAGTT111120.11614199131882513No Hit
GGAATACCGGGTGCTGTAGGCTTT109980.11495046980961472No Hit
CCCATAAAGTAGAAAGCACTA106990.1118253388337032No Hit
TGTAAACATCCTACACTCAGCT106820.11164765580162798No Hit
CACTAGATTGTGAGCTCCTGGAA106300.11110415476233901No Hit
CTCCCACTGCTTCACTTGACTAGCCT104880.10961997884735764No Hit
TATTGCACTTGTCCCGGCCTGTAG103580.10826122624913524No Hit
TCCTGTCTGAGCGTCGCT101510.10609767403504265No Hit
AACATTCAACGCTGTCGGTGA100890.1054496535651212No Hit
GACCCGCGGGCGCTCTCCAGTCCT100430.10496886418421174No Hit
TAGCAGCACATCATGGTTTACA96190.10053724032539407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)