FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9365123
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8924009.528972550600777No Hit
CAACGGAATCCCAAAAGCAGCTG8328198.892771616560722No Hit
TTCAAGTAATCCAGGATAGGCT3592703.8362550070084502No Hit
TAGCAGCACGTAAATATTGGCG3233533.452736285471104No Hit
AAGCTGCCAGTTGAAGAACTGT3149243.362732128558269No Hit
AACATTCAACGCTGTCGGTGAGT2901963.0986886130593265No Hit
CCCCCACTGCTAAATTTGACTG2634402.812990283202901No Hit
AACATTCAACGCTGTCGGTGAG1815751.9388426612229226No Hit
TATTGCACTTGTCCCGGCCTGTAT1665241.7781293422414208No Hit
TATTGCACTTGTCCCGGCCTGTAA1648111.759838071534138No Hit
TAGCTTATCAGACTGATGTTGACT1634381.7451772923858022No Hit
TAGCTTATCAGACTGATGTTGAC1631981.7426145924618397No Hit
TATTGCACTTGTCCCGGCCTGT1201651.283111818179003No Hit
TAGCTTATCAGACTGATGTTGA1179741.2597165034564948No Hit
TGAGGTAGTAGGTTGTATAGTT1032781.1027938447791876No Hit
CATAAAGTAGAAAGCACTACT759310.810784866360004No Hit
TGTAAACATCCTTGACTGGAAGCT744640.795120363074783No Hit
TCGTACGACTCTTAGCGG734700.7845065142230381No Hit
TATTGCACTTGTCCCGGCCTGTA714530.7629691569454027No Hit
AGCAGCATTGTACAGGGCTATGA658750.7034077395459729No Hit
CCCATAAAGTAGAAAGCACTAC640590.6840166434546562No Hit
TGAGGTAGTAGATTGTATAGTT561900.5999921196977338No Hit
TTCAAGTAATTCAGGATAGGTT550370.5876804821463637No Hit
ACTGGACTTGGAGTCAGAAGGC541540.5782518820094514No Hit
CAACGGAATCCCAAAAGCAGCTGT516880.5519201402907361No Hit
TGTAAACATCCCCGACTGGAAGCT458530.4896144983893965No Hit
CACCCGGCTGTGTGCACATGTGC448430.4788298028760541No Hit
CATTGCACTTGTCTCGGTCTGA409460.4372179628607121No Hit
CCCATAAAGTAGAAAGCACT404290.4316974801078427No Hit
TTCTCACTACTGCACTTGACTAG332300.3548271603053158No Hit
CTCCCACTGCTTCACTTGACTAG331550.35402631657907746No Hit
CTTCTCACTACTGCACTTGACTAG330980.35341767534713636No Hit
CAACACCAGTCGATGGGCTGT330880.353310896183638No Hit
CAACGGAATCCCAAAAGCAGCT317310.3388209636968997No Hit
CGCCTGTCTGAGCGTCGCT316020.33744351248776977No Hit
CCCCCCACTGCTAAATTTGACTGGCT265770.2837869828298037No Hit
TGAGGTAGTAGTTTGTACAGTT257060.2744865176890896No Hit
TGTAAACATCCTACACTCTCAGCT256030.2733866923050557No Hit
CCCCCACTGCTAAATTTGACTGGCTT253870.2710802623734894No Hit
CACTAGATTGTGAGCTCCTGGA243710.2602314993620479No Hit
ATCCCGGACGAGCCCCCA233410.24923324552170856No Hit
AGCTCGGTCTGAGGCCCCTCAGT225950.24126751992472498No Hit
TAGCTTATCAGACTGATGTTGACA217790.23255434018325227No Hit
TATTGCACTTGTCCCGGCCTGTT212270.2266601303581384No Hit
CAAAGAATTCTCCTTTTGGGCT211790.22614759037334586No Hit
CACTAGATTGTGAGCTCCTGGAA211400.22573115163570193No Hit
AACCGGGCGGAAACACCA205240.21915355516419804No Hit
AACGGAATCCCAAAAGCAGCTG198200.21163630205390788No Hit
TTCAAGTAATCCAGGATAGGCTT198110.21154020080675928No Hit
TATTGCACTTGTCCCGGCCTGTAG192830.20590226097404168No Hit
AACATTCAACGCTGTCGGTGAGTT191080.20403362561281896No Hit
GCCTGTCTGAGCGTCGCT186320.19895093743029324No Hit
CCCCCCACTGCTAAATTTGACTGG182040.19438078923256No Hit
TAGCAGCACGTAAATATTGGC180550.19278977969643324No Hit
CAAAGAATTCTCCTTTTGGGCTT171800.18344660289031978No Hit
TGAGGTAGTAGTTTGTGCTGTT168960.18041407464696405No Hit
TTCAAGTAATCCAGGATAGGC165730.17696510766596446No Hit
CTACGCCTGTCTGAGCGTCGCT162950.17399664692070782No Hit
TATTGCACTTGTCCCGGCCTGTTT162940.17398596900435798No Hit
TGTCAGTTTGTCAAATACCCCAA156120.16670363005376437No Hit
TCGTTTCCCGGGCGGCGCACCA155590.16613770048722265No Hit
ACCCCCCACTGCTAAATTTGACTG154350.164813638859842No Hit
CTCCCACTGCTTCACTTGACTAGCCT139180.14861523975712865No Hit
AGCAGCATTGTACAGGGCTATGT138210.1475794818711938No Hit
ACCGGGTGCTGTAGGCTTA135130.14429068363544184No Hit
TGTAAACATCCCCGACTGGAAGC134780.1439169565631973No Hit
ACTGGACTTGGAGTCAGAAGGCT134470.14358594115635215No Hit
ACCGGGTGCTGTAGGCTT133650.14271035201566495No Hit
TCGTTTCCCGGCCAATGCACCA131930.14087375040349176No Hit
CCCATAAAGTAGAAAGCACTA131080.13996612751375503No Hit
TTCACAGTGGCTAAGTTCCGC130280.13911189420576753No Hit
ACTGGACTTGGAGTCAGAAGGCA128000.13667732927800308No Hit
TACGCCTGTCTGAGCGTCGCT127450.13609004387876167No Hit
TTCACAGTGGCTAAGTTCTGC121790.13004634322474995No Hit
TATTGCACTTGTCCCGGCCTGA120590.12876499326276866No Hit
TCAGTGCACTACAGAACTTTGT120270.12842329993957366No Hit
TATTGCACTTGTCCCGGCCTGTAAT119060.12713127206124256No Hit
TGTCAGTTTGTCAAATACCCCA116530.12442975922473201No Hit
CAAAGTGCTGTTCGTGCAGGT114580.12234756553651245No Hit
ACCACAGGGTAGAACCACGGAC114450.12220875262396447No Hit
TTATAAAGCAATGAGACTGATT112750.12039350684449099No Hit
TTCACAGTGGCTAAGTTCCG109860.11730758901938607No Hit
CAAAGTGCTGTTCGTGCAGGTAG108290.11563115615246056No Hit
ATCCCACCGCTGCCACCA107130.11439251785587867No Hit
CCCCCCACTGCTAAATTTGACTGT105550.11270540707260332No Hit
TGAGGTAGTAGGTTGTGTGGTT104360.11143473502697188No Hit
TAGCAGCACGTAAATATTGGCGT101200.10806051346042118No Hit
AACATTCAACGCTGTCGGTGA101110.10796441221327259No Hit
ACCGGGTGCTGTAGGCTTT99400.10613848851744925No Hit
AGAGGTAGTAGGTTGCATAGTT98510.10518815396231315No Hit
AGCAGCATTGTACAGGGCTATG98030.10467561397752065No Hit
TTCACCACCTTCTCCACCCAGC97640.10425917523987673No Hit
ACTGGACTTGGAGTCAGAAGG96490.10303121485964466No Hit
ATCACATTGCCAGGGATTTCC95980.10248664112580262No Hit
ACGCCTGTCTGAGCGTCGCT94740.10116257949842197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)