FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9855520
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5717625.801439193467214No Hit
CAACGGAATCCCAAAAGCAGCTG5612145.694412877250516No Hit
TTCAAGTAATCCAGGATAGGCT4115994.176329610208289No Hit
AAGCTGCCAGTTGAAGAACTGT3631703.6849400133123362No Hit
TAGCAGCACGTAAATATTGGCG3369783.4191803172232413No Hit
TAGCTTATCAGACTGATGTTGAC3208003.255028653992889No Hit
TAGCTTATCAGACTGATGTTGA2497752.5343665275906297No Hit
TAGCTTATCAGACTGATGTTGACT2392702.4277765150900206No Hit
CCCCCACTGCTAAATTTGACTG2265102.2983059239898047No Hit
GGAATACCGGGTGCTGTAGGCTTT2199212.231449989447538No Hit
AACATTCAACGCTGTCGGTGAGT2166722.1984836923876165No Hit
TGAGGTAGTAGGTTGTATAGTT1803991.8304361413705215No Hit
CATAAAGTAGAAAGCACTACT1479151.5008340503595956No Hit
GGAATACCGGGTGCTGTAGGCTT1429361.450314138675585No Hit
AACATTCAACGCTGTCGGTGAG1288501.3073891585629172No Hit
TGAGGTAGTAGATTGTATAGTT1193901.2114023410231018No Hit
TGTAAACATCCTTGACTGGAAGCT1191761.2092309690407No Hit
TGAATACCGGGTGCTGTAGGCTTT1042801.058087244508661No Hit
TTCAAGTAATTCAGGATAGGTT1012251.027089387470169No Hit
TATTGCACTTGTCCCGGCCTGTAA811890.8237921489682939No Hit
TATTGCACTTGTCCCGGCCTGT788700.800262188093576No Hit
TATTGCACTTGTCCCGGCCTGTAT722820.7334164001493579No Hit
TGAATACCGGGTGCTGTAGGCTT676740.6866608763413803No Hit
AGCAGCATTGTACAGGGCTATGA638030.6473833952952255No Hit
TGAGGTAGTAGTTTGTACAGTT469570.4764538045684043No Hit
CCCATAAAGTAGAAAGCACTAC448080.4546487653619495No Hit
CATTGCACTTGTCTCGGTCTGA426900.4331582706950014No Hit
TATTGCACTTGTCCCGGCCTGTA419920.42607594525707426No Hit
ACTGGACTTGGAGTCAGAAGGC415430.42152012273325No Hit
TGTAAACATCCCCGACTGGAAGCT411970.4180093998084322No Hit
TGTAAACATCCTACACTCTCAGCT401110.4069901943276459No Hit
TAGCTTATCAGACTGATGTTGACA365310.3706653733136354No Hit
TTCTCACTACTGCACTTGACTAG355920.3611377177459941No Hit
CAACGGAATCCCAAAAGCAGCTGT346610.3516912349627417No Hit
GGAATACCGGGTGCTGTAGGCTTA344910.34996631329447864No Hit
AACCGGGCGGAAACACCA323370.3281105410977807No Hit
TTATAAAGCAATGAGACTGATT301150.3055648002337776No Hit
TGAGGTAGTAGTTTGTGCTGTT278520.28260304884978166No Hit
CCCATAAAGTAGAAAGCACT267560.27148237738850917No Hit
CGCCTGTCTGAGCGTCGCT265300.26918924622952417No Hit
GCCTGTCTGAGCGTCGCT261250.2650798740198386No Hit
CAACACCAGTCGATGGGCTGT259070.2628679156452424No Hit
TCGTACGACTCTTAGCGG251690.25537972628537103No Hit
CTTCTCACTACTGCACTTGACTAG238480.24197607026316217No Hit
TGTCAGTTTGTCAAATACCCCA230490.23386893842232576No Hit
TTCAAGTAATCCAGGATAGGCTT227470.23080466581164666No Hit
CACCCGGCTGTGTGCACATGTGC217630.22082041333181812No Hit
TCAGTGCACTACAGAACTTTGT216380.21955208857574235No Hit
CCCCCACTGCTAAATTTGACTGGCTT205880.20889816062470576No Hit
TTAATGCTAATCGTGATAGGGGTT202330.20529611831745054No Hit
CAAAGAATTCTCCTTTTGGGCT193510.19634681883857982No Hit
CCCCCCACTGCTAAATTTGACTGGCT192890.19571772975956622No Hit
CTCCCACTGCTTCACTTGACTAG192560.19538289202396222No Hit
GGAATACCGGGTGCTGTAGGCT191560.19436823221910157No Hit
TGTCAGTTTGTCAAATACCCCAA191490.19429720603276135No Hit
CTACGCCTGTCTGAGCGTCGCT186560.18929493319479845No Hit
TGTAAACATCCTACACTCAGCT182660.185337759955842No Hit
TAGCAGCACGTAAATATTGGC181440.18409987499391206No Hit
TTCAAGTAATCCAGGATAGGC179980.18261847167881554No Hit
TTCACAGTGGCTAAGTTCCGC168460.1709295907268211No Hit
TGAATACCGGGTGCTGTAGGCTTA168290.1707570985599948No Hit
AACATTCAACGCTGTCGGTGAGTT167510.16996566391220352No Hit
CAACGGAATCCCAAAAGCAGCT164090.16649552737958018No Hit
TTCACAGTGGCTAAGTTCCG163230.16562291994740005No Hit
AACGGAATCCCAAAAGCAGCTG162770.16515617643716415No Hit
TGTCAGTTTGTCAAATACCCC160840.16319788301378313No Hit
CCCCCCACTGCTAAATTTGACTGG156760.15905807100995178No Hit
CAAAGAATTCTCCTTTTGGGCTT155580.15786077244021623No Hit
TACGCCTGTCTGAGCGTCGCT152820.1550603113788009No Hit
TGAGGTAGTAGGTTGTATAGT146080.1482215042940403No Hit
TGAGGTAGTAAGTTGTATTGTT143560.14566456158579152No Hit
AGCAGCATTGTACAGGGCTATGT138640.14067243534587723No Hit
AGCTCGGTCTGAGGCCCCTCAGT135110.13709068623471923No Hit
TTCACAGTGGCTAAGTTCTGC134550.13652247674399726No Hit
CACTAGATTGTGAGCTCCTGGA130840.13275808886796434No Hit
TATTGCACTTGTCCCGGCCTGTT129940.1318448950435898No Hit
AGAGGTAGTAGGTTGCATAGTT129090.13098243420945824No Hit
TAGCAGCACGTAAATATTGGCGT126270.1281210935597513No Hit
TCAGTGCACTACAGAACTTTGTC121580.12336233907495496No Hit
AGAATTACTCTGAGACCT117860.11958780460087343No Hit
CAAAGTGCTGTTCGTGCAGGTAG116480.11818757407016575No Hit
TGAGGTAGTAGGTTGTGTGGTT114830.11651338539214573No Hit
ACCGGGTGCTGTAGGCTT112880.1145347987726675No Hit
TCCTGTCTGAGCGTCGCT112700.1143521600077926No Hit
TGTAAACATCCCCGACTGGAAGC112480.11412893485072326No Hit
TAGCTTATCAGACTGATGTTG111330.11296207607513352No Hit
TGTAAACATCCTTGACTGGAAGC110690.11231269380002273No Hit
CAAAGTGCTGTTCGTGCAGGT110190.11180536389759242No Hit
TGTCAGTTTGTCAAATACCC109210.110810997288829No Hit
TGTCAGTTTGTCAAATACC106650.1082134681883858No Hit
TGAGAACTGAATTCCATAGGCTGT105890.10744232673669171No Hit
CACTAGATTGTGAGCTCCTGGAA104950.10648854652012274No Hit
AGCAGCATTGTACAGGGCTATG104030.10555505949965097No Hit
TAGCAGCACATAATGGTTTGT104030.10555505949965097No Hit
TTCACAGTGGCTAAGTTCTG103090.10460127928308197No Hit
ATCACATTGCCAGGGATTTCC102630.10413453577284608No Hit
ATACCGGGTGCTGTAGGCTTT98890.10033970810266733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)