FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8302320
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5770486.950442767804661No Hit
CAACGGAATCCCAAAAGCAGCTG4836175.825082627506529No Hit
AAGCTGCCAGTTGAAGAACTGT3834254.618287418456528No Hit
TTCAAGTAATCCAGGATAGGCT3135233.7763299896896285No Hit
TAGCAGCACGTAAATATTGGCG3004243.618554813594273No Hit
TAGCTTATCAGACTGATGTTGAC2805983.379754092831883No Hit
AACATTCAACGCTGTCGGTGAGT2262442.7250696190944215No Hit
TAGCTTATCAGACTGATGTTGACT2040862.4581803640428217No Hit
CCCCCACTGCTAAATTTGACTG1896002.283699014251438No Hit
TAGCTTATCAGACTGATGTTGA1625141.9574528565509401No Hit
TGTAAACATCCTTGACTGGAAGCT1235851.4885598242418985No Hit
AACATTCAACGCTGTCGGTGAG1217871.4669032270497884No Hit
CATAAAGTAGAAAGCACTACT1078791.2993837867005849No Hit
TGAGGTAGTAGGTTGTATAGTT1034841.246446776322763No Hit
TATTGCACTTGTCCCGGCCTGTAA996771.200592123647366No Hit
TATTGCACTTGTCCCGGCCTGTAT889621.071531812794496No Hit
TGAGGTAGTAGATTGTATAGTT792900.9550342554852138No Hit
TATTGCACTTGTCCCGGCCTGT688270.8290092407905261No Hit
TTCAAGTAATTCAGGATAGGTT599440.7220150512146003No Hit
AGCAGCATTGTACAGGGCTATGA597090.719184517098835No Hit
GCCTGTCTGAGCGTCGCT570400.6870368764393567No Hit
TGTCAGTTTGTCAAATACCCCAA491070.5914852715867372No Hit
TGTAAACATCCCCGACTGGAAGCT471270.5676365160581621No Hit
CCCATAAAGTAGAAAGCACTAC462750.55737432428526No Hit
TGTAAACATCCTACACTCTCAGCT454630.5475939255533393No Hit
TATTGCACTTGTCCCGGCCTGTA408540.49207932240626717No Hit
CGCCTGTCTGAGCGTCGCT405070.4878997677757542No Hit
TTCTCACTACTGCACTTGACTAG389150.4687244047446979No Hit
TGTCAGTTTGTCAAATACCCCA369120.4445986182175584No Hit
TGAGGTAGTAGTTTGTACAGTT367900.44312914944256543No Hit
TAGCTTATCAGACTGATGTTGACA366340.4412501565827383No Hit
ACTGGACTTGGAGTCAGAAGGC345460.41610055984351363No Hit
CAACGGAATCCCAAAAGCAGCTGT331280.399020996540726No Hit
CTTCTCACTACTGCACTTGACTAG303420.3654641112363773No Hit
CATTGCACTTGTCTCGGTCTGA286550.3451444897329903No Hit
CCCATAAAGTAGAAAGCACT262340.31598396592759614No Hit
CAAAGAATTCTCCTTTTGGGCT224630.2705629269890826No Hit
TGTAAACATCCTACACTCAGCT214570.2584458320084025No Hit
CACCCGGCTGTGTGCACATGTGC204890.24678644041665462No Hit
CCCCCACTGCTAAATTTGACTGGCTT200820.24188419622466975No Hit
CAACACCAGTCGATGGGCTGT199340.2401015619730389No Hit
TGTCAGTTTGTCAAATACCCC184910.22272087801963789No Hit
TTAATGCTAATCGTGATAGGGGTT184870.22267269871553974No Hit
TTATAAAGCAATGAGACTGATT181530.21864972682334574No Hit
CAAAGAATTCTCCTTTTGGGCTT180970.2179752165659719No Hit
AACATTCAACGCTGTCGGTGAGTT180970.2179752165659719No Hit
TCGTACGACTCTTAGCGG178040.2144460825407838No Hit
TGAGGTAGTAGTTTGTGCTGTT177650.21397633432582702No Hit
CCCCCCACTGCTAAATTTGACTGGCT172940.20830322126827197No Hit
CTACGCCTGTCTGAGCGTCGCT171370.2064121835824203No Hit
TTCAAGTAATCCAGGATAGGCTT168880.20341302190231164No Hit
TAGCAGCACGTAAATATTGGC168710.20320825985989457No Hit
CTCCCACTGCTTCACTTGACTAG157490.18969396506036867No Hit
AACCGGGCGGAAACACCA157100.18922421684541188No Hit
TGTAAACATCCCCGACTGGAAGC154130.18564690351612562No Hit
TTCACAGTGGCTAAGTTCCGC153170.18449060021777045No Hit
TAGCAGCACGTAAATATTGGCGT153010.18429788300137795No Hit
CCCCCCACTGCTAAATTTGACTGG151850.1829006831825321No Hit
TACGCCTGTCTGAGCGTCGCT151520.18250320392372255No Hit
TTCAAGTAATCCAGGATAGGC145640.1754208462212972No Hit
TGAGAACTGAATTCCATAGGCTGT139030.16745921621908094No Hit
TTCACAGTGGCTAAGTTCCG134620.1621474479422619No Hit
ACCCCCCACTGCTAAATTTGACTG123710.14900654274949654No Hit
TTCACAGTGGCTAAGTTCTGC121960.14689869819520326No Hit
TCAGTGCACTACAGAACTTTGT118550.14279141252083755No Hit
TGATGTAGTAGGTTGTATAGTT117950.1420687229593656No Hit
AGCAGCATTGTACAGGGCTATGT116550.14038244731593097No Hit
TAGCAGCACATCATGGTTTACA115250.1388166199327417No Hit
TATTGCACTTGTCCCGGCCTGTT114060.13738328563582228No Hit
AACGGAATCCCAAAAGCAGCTG113990.13729897185365056No Hit
ACGCCTGTCTGAGCGTCGCT110150.13267375866022993No Hit
CAACGGAATCCCAAAAGCAGCT109750.1321919656192486No Hit
CAAAGTGCTGTTCGTGCAGGTAG108500.13068636236618197No Hit
TATTGCACTTGTCCCGGCCTGTAG108410.1305779589319612No Hit
ATCACATTGCCAGGGATTTCCA108300.1304454658456913No Hit
ATCACATTGCCAGGGATTTCC104790.1262177319110803No Hit
TAGCAGCACATAATGGTTTGT104060.1253384596112894No Hit
AGCTCGGTCTGAGGCCCCTCAGT99770.12017122924676475No Hit
TGTAAACATCCTTGACTGGAAGC99600.11996646720434771No Hit
AGAGGTAGTAGGTTGCATAGTT98790.11899083629636054No Hit
TGTCAGTTTGTCAAATACCC95930.11554601605334412No Hit
TGAGGTAGTAGGTTGTATAGT93810.11299251293614314No Hit
GGAATACCGGGTGCTGTAGGCTTT93230.11229391302672025No Hit
TGAGGTAGTAGGTTGTGTGGTT91980.11078830977365364No Hit
TGAGAACTGAATTCCATGGGTT91870.11065581668738378No Hit
TGATGTAGTAGATTGTATAGTT91610.1103426512107459No Hit
GCGTTCGCGCTTTCCCCTG91030.109644051301323No Hit
ACTGGACTTGGAGTCAGAAGGCA90800.10936702030275874No Hit
ATCCCACCGCTGCCACCA90410.10889727208780198No Hit
CAAAGTGCTGTTCGTGCAGGT90150.1085841066111641No Hit
AGCAGCATTGTACAGGGCTATG86390.10405525202593974No Hit
CAGTGCAATAGTATTGTCAAAGC86380.10404320719991521No Hit
CTCCCACTGCTTCACTTGACTAGCCT86310.10395889341774348No Hit
CACTAGATTGTGAGCTCCTGGAA85520.10300735216180537No Hit
CACTAGATTGTGAGCTCCTGGA85440.10291099355360911No Hit
ACCACAGGGTAGAACCACGGAC83890.1010440455198065No Hit
ACCGGGTGCTGTAGGCTT83700.10081519382534039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)