FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11416054
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG199111817.441385613627965No Hit
CCCCCCACTGCTAAATTTGACTG133402511.68551760529514No Hit
CCCCCACTGCTAAATTTGACTG4458013.9050358381276054No Hit
TTCAAGTAATCCAGGATAGGCT3059852.68030442042408No Hit
TCGTACGACTCTTAGCGG2812862.463951204155131No Hit
AACATTCAACGCTGTCGGTGAG2793462.446957591476004No Hit
TAGCAGCACGTAAATATTGGCG1933591.6937463680532696No Hit
TATTGCACTTGTCCCGGCCTGTAA1555691.3627213045768705No Hit
AAGCTGCCAGTTGAAGAACTGT1548051.3560289746351935No Hit
TGAGGTAGTAGATTGTATAGTT1409541.2346998358627246No Hit
TATTGCACTTGTCCCGGCCTGT1370711.2006863317219767No Hit
TATTGCACTTGTCCCGGCCTGTAT1349201.1818444446741405No Hit
TAGCTTATCAGACTGATGTTGAC1337741.171805949761625No Hit
TGAGGTAGTAGGTTGTATAGTT1080690.9466405817631908No Hit
TGAGAACTGAATTCCATGGGTT964120.8445299925876315No Hit
CACCCGGCTGTGTGCACATGTGC962840.8434087645345756No Hit
TAGCTTATCAGACTGATGTTGACT929160.8139064513885446No Hit
GCCTGTCTGAGCGTCGCT926080.811208496385879No Hit
TAGCTTATCAGACTGATGTTGA856080.7498913372343894No Hit
AACATTCAACGCTGTCGGTGAGT836130.7324159468762148No Hit
CAACGGAATCCCAAAAGCAGCTGT749760.6567593320774412No Hit
TATTGCACTTGTCCCGGCCTGTA746230.6536671953373732No Hit
TTAATGCTAATCGTGATAGGGGTT712630.6242349589446581No Hit
GGAATACCGGGTGCTGTAGGCTTT647090.5668245787905348No Hit
GGAATACCGGGTGCTGTAGGCTT543270.4758824721747112No Hit
CAACGGAATCCCAAAAGCAGCT534540.4682353464691039No Hit
AGCAGCATTGTACAGGGCTATGA529940.46420593315343467No Hit
TGAGAACTGAATTCCATAGGCTGT513700.44998035223028904No Hit
TGAGGTAGTAGTTTGTACAGTT468240.41015923715847874No Hit
TGTAAACATCCTTGACTGGAAGCT461670.4044041837924032No Hit
AACGGAATCCCAAAAGCAGCTG458010.40119817232819677No Hit
CCCCCACTGCTAAATTTGACTGGCTT457690.40091786531493284No Hit
TTCAAGTAATTCAGGATAGGTT447910.3923509822220532No Hit
TTCTCACTACTGCACTTGACTAG419210.3672109469699425No Hit
CATTGCACTTGTCTCGGTCTGA418270.36638754511847965No Hit
ACTGGACTTGGAGTCAGAAGGC387410.33935543752683717No Hit
TCGTTTCCCGGCCAATGCACCA381500.3341785173756186No Hit
CTCCCACTGCTTCACTTGACTAG361080.3162914260917126No Hit
ACCCCCCACTGCTAAATTTGACTG338600.2965998584099199No Hit
CCCATAAAGTAGAAAGCACTAC327880.2872095734655775No Hit
TGTAAACATCCCCGACTGGAAGCT322370.2823830370809388No Hit
CTTCTCACTACTGCACTTGACTAG312590.27381615398805925No Hit
CATAAAGTAGAAAGCACTACT288830.2530033582532108No Hit
CCCATAAAGTAGAAAGCACT266370.2333293097597471No Hit
CCCCCCACTGCTAAATTTGACTGG265750.2327862149215482No Hit
CACTAGATTGTGAGCTCCTGGA249400.21846427846259311No Hit
CTCCCACTGCTTCACTTGACTAGCCT229580.20110276282855705No Hit
CCCCCCACTGCTAAATTTGACTGGCT227710.19946471871979582No Hit
CAACACCAGTCGATGGGCTGT220130.19282494634310596No Hit
CACTAGATTGTGAGCTCCTGGAA219080.1919051889558336No Hit
ACCGGGTGCTGTAGGCTT217320.19036350038288186No Hit
TGATGTAGTAGATTGTATAGTT203970.17866944217327635No Hit
AACCGGGCGGAAACACCA198800.17414073199023059No Hit
TATTGCACTTGTCCCGGCCTGTAG193270.16929667641726293No Hit
TAGCAGCACGTAAATATTGGC192030.1682104867408651No Hit
TAGCTTATCAGACTGATGTTGACA189370.1658804346931085No Hit
TCGTTTCCCGGCCAACGCACCA188850.16542493579655457No Hit
AGCTCGGTCTGAGGCCCCTCAGT188050.16472416826339467No Hit
TATTGCACTTGTCCCGGCCTGTT181300.15881144220235818No Hit
CCCATAAAGTAGAAAGCACTA179380.15712960012277447No Hit
CAAAGAATTCTCCTTTTGGGCT172380.1509978842076255No Hit
TCGTTTCCCGGGCGGCGCACCA165200.14470849559751559No Hit
TTCACAGTGGCTAAGTTCCGC162370.14222953044896247No Hit
TGATGTAGTAGGTTGTATAGTT159200.13945273909881645No Hit
ACCGGGTGCTGTAGGCTTT155860.13652703464787397No Hit
TATTGCACTTGTCCCGGCCTGTAC154150.1350291440457447No Hit
TGAGGTAGTAGATTGTATAGT148370.12996609861866457No Hit
TGTAAACATCCTACACTCTCAGCT143150.12539359046479634No Hit
AATCCTTGGAACCTAGGTGTGAGT142720.1250169279157229No Hit
GACCCGCGGGCGCTCTCCAGTCCT137960.1208473610934216No Hit
TCGATTCCCGGCCAACGCACCA134520.1178340607008341No Hit
ATGCACCTGGGCAAGGATTCTGA133380.11683546696608128No Hit
AATGCACCCGGGCAAGGATTCT131540.11522370163981355No Hit
TGATAACTGAATTCCATGGGTT128860.11287613040372793No Hit
ACTGGACTTGGAGTCAGAAGGCT128380.11245566988383202No Hit
TGAGAACTGAATTCCATGGGTTG126830.11109793278833474No Hit
GAATACCGGGTGCTGTAGGCTTT126820.11108917319417025No Hit
TTCAAGTAATCCAGGATAGGC125800.11019569458939141No Hit
CAAAGTGCTGTTCGTGCAGGTAG125510.10994166635862095No Hit
TTCACAGTGGCTAAGTTCTGC122900.10765541228168682No Hit
ACTGGACTTGGAGTCAGAAGGCA120870.10587721466629363No Hit
TGTAAACATCCCCGACTGGA118370.103687316125169No Hit
TGAGGTAGTAGTTTGTGCTGTT115020.10075285208006197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)