FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7744912
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89700511.581861743554994No Hit
CAACGGAATCCCAAAAGCAGCTG5932997.66050020968605No Hit
TTCAAGTAATCCAGGATAGGCT3029973.9122071367628193No Hit
AAGCTGCCAGTTGAAGAACTGT2571653.320437985609133No Hit
CCCCCACTGCTAAATTTGACTG2424163.1300032847371284No Hit
TAGCAGCACGTAAATATTGGCG2185132.821374858745974No Hit
AACATTCAACGCTGTCGGTGAGT1943402.5092602730670146No Hit
TAGCTTATCAGACTGATGTTGAC1585372.0469825867614766No Hit
TAGCTTATCAGACTGATGTTGACT1407091.8167927537459432No Hit
AACATTCAACGCTGTCGGTGAG1103601.424935493134073No Hit
TGAGGTAGTAGGTTGTATAGTT966751.248238843772531No Hit
TATTGCACTTGTCCCGGCCTGTAA892871.1528471853521383No Hit
TGTAAACATCCTTGACTGGAAGCT883301.1404906860142503No Hit
TAGCTTATCAGACTGATGTTGA836381.0799089776617217No Hit
TATTGCACTTGTCCCGGCCTGTAT772600.9975581388142305No Hit
CCCATAAAGTAGAAAGCACTAC700290.9041936176937839No Hit
CATAAAGTAGAAAGCACTACT649020.8379953187331244No Hit
TGAGGTAGTAGATTGTATAGTT520000.6714085324662178No Hit
AGCAGCATTGTACAGGGCTATGA494440.6384062207549938No Hit
TATTGCACTTGTCCCGGCCTGT483700.6245390522190568No Hit
CAACGGAATCCCAAAAGCAGCTGT445710.5754874942413807No Hit
TGTAAACATCCCCGACTGGAAGCT426580.5507874072681523No Hit
CCCCCACTGCTAAATTTGACTGGCTT426280.5504000561917295No Hit
ACTGGACTTGGAGTCAGAAGGC417260.5387537004939501No Hit
TGTAAACATCCTACACTCTCAGCT371950.48025077625155715No Hit
TATTGCACTTGTCCCGGCCTGTA364400.4705024408282496No Hit
TTCAAGTAATTCAGGATAGGTT332280.4290300522459132No Hit
TGTCAGTTTGTCAAATACCCCAA293340.37875188252623143No Hit
CCCCCCACTGCTAAATTTGACTGGCT288060.37193450358118985No Hit
CAAAGAATTCTCCTTTTGGGCT261220.33727949394389506No Hit
CTTCTCACTACTGCACTTGACTAG257920.33301863210324406No Hit
GCCTGTCTGAGCGTCGCT248880.3213464530003698No Hit
TAGCTTATCAGACTGATGTTGACA244170.31526504110053155No Hit
TTCTCACTACTGCACTTGACTAG243190.3139996942508837No Hit
CTCCCACTGCTTCACTTGACTAG239720.3095193334669264No Hit
CCCCCCACTGCTAAATTTGACTGG236650.30555544078486624No Hit
AACCGGGCGGAAACACCA227420.2936379393335909No Hit
TGAGGTAGTAGTTTGTACAGTT221970.28660106144524305No Hit
CATTGCACTTGTCTCGGTCTGA216330.27931886120849403No Hit
ACCCCCCACTGCTAAATTTGACTG216240.2792026558855672No Hit
CCCATAAAGTAGAAAGCACT199420.25748517220079453No Hit
CTCCCACTGCTTCACTTGACTAGCCT195910.2529531646066476No Hit
CGCCTGTCTGAGCGTCGCT194900.2516490826493574No Hit
TGTCAGTTTGTCAAATACCCCA182160.2351995736039351No Hit
TCGTACGACTCTTAGCGG173970.22462488921759213No Hit
CTACGCCTGTCTGAGCGTCGCT168040.21696824960696776No Hit
AACATTCAACGCTGTCGGTGAGTT162660.2100217536364519No Hit
TTCACAGTGGCTAAGTTCCGC158250.20432769281303648No Hit
CAAAGAATTCTCCTTTTGGGCTT158210.20427604600284677No Hit
TAGCAGCACGTAAATATTGGC157730.20365628428057028No Hit
CAACGGAATCCCAAAAGCAGCT152020.1962837021259893No Hit
ATCACATTGCCAGGGATTTCCA149450.1929653945713005No Hit
TGAGGTAGTAGTTTGTGCTGTT145710.1881364178185627No Hit
TGTAAACATCCTACACTCAGCT145470.18782653695742443No Hit
TAGCAGCACGTAAATATTGGCGT145300.18760703801411815No Hit
ATCACATTGCCAGGGATTTCC142780.1843532889721665No Hit
CAACACCAGTCGATGGGCTGT139780.1804797782079383No Hit
CACCCGGCTGTGTGCACATGTGC138060.17825896536978084No Hit
AACGGAATCCCAAAAGCAGCTG130800.16888506932034864No Hit
TTCAAGTAATCCAGGATAGGCTT130600.1686268352694001No Hit
ACTGGACTTGGAGTCAGAAGGCA129810.16760681076815334No Hit
TTCAAGTAATCCAGGATAGGC128320.16568296708858668No Hit
TAGCAGCACATCATGGTTTACA128210.16554093836056497No Hit
TACGCCTGTCTGAGCGTCGCT118810.15340393796598334No Hit
TCCTGTCTGAGCGTCGCT112000.14461106853118538No Hit
TGTAAACATCCCCGACTGGAAGC110790.14304875252294666No Hit
TTCACAGTGGCTAAGTTCCG109790.14175758226820395No Hit
CACTAGATTGTGAGCTCCTGGAA108820.1405051471211035No Hit
TTCACAGTGGCTAAGTTCTGC107350.13860712684663168No Hit
CACTAGATTGTGAGCTCCTGGA106600.13763874915557467No Hit
ACTGGACTTGGAGTCAGAAGGCT100410.12964640527871718No Hit
AGCAGCATTGTACAGGGCTATGT100300.12950437655069547No Hit
TCGTTTCCCGGCCAATGCACCA97950.12647012645205008No Hit
TATTGCACTTGTCCCGGCCTGTT97380.1257341594068467No Hit
TATTGCACTTGTCCCGGCCTGTAG95680.12353916997378407No Hit
TGAGGTAGTAGGTTGTGTGGTT94680.12224799971904136No Hit
CCCATAAAGTAGAAAGCACTA92600.11956236558917648No Hit
TTATAAAGCAATGAGACTGATT90370.11668305592110019No Hit
TCAGTGCACTACAGAACTTTGT89610.11570176652749573No Hit
CAAAGTGCTGTTCGTGCAGGTAG88640.11444933138039527No Hit
CCCCCACTGCTAAATTTGACTGGCT86870.11216396002950066No Hit
TATTGCACTTGTCCCGGCCTGTAAT83180.1073995417895No Hit
AGAGGTAGTAGGTTGCATAGTT82850.10697345560543489No Hit
ACTGGACTTGGAGTCAGAAGG81700.10548860981248076No Hit
ATGCACCTGGGCAAGGATTCTGA80190.10353894272781924No Hit
CCCCCACTGCTAAATTTGACTGGCTA79430.10255765333421477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)