FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9431676
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8197938.69191223277814No Hit
CAACGGAATCCCAAAAGCAGCTG6969667.389630432597557No Hit
AAGCTGCCAGTTGAAGAACTGT3609393.826880821605831No Hit
AACATTCAACGCTGTCGGTGAGT3173883.3651283186572565No Hit
TTCAAGTAATCCAGGATAGGCT2744922.9103204987109397No Hit
TAGCAGCACGTAAATATTGGCG2741442.9066308045356943No Hit
CCCCCACTGCTAAATTTGACTG2474212.6232983406130574No Hit
AACATTCAACGCTGTCGGTGAG1585301.6808253379356968No Hit
GCCTGTCTGAGCGTCGCT1183611.2549307249316028No Hit
TATTGCACTTGTCCCGGCCTGT1128941.196966477644058No Hit
TAGCTTATCAGACTGATGTTGAC1125861.1937008862475769No Hit
TATTGCACTTGTCCCGGCCTGTAA929660.9856784732639247No Hit
TATTGCACTTGTCCCGGCCTGTAT870790.9232611467993599No Hit
TAGCTTATCAGACTGATGTTGA867100.9193487986652636No Hit
CGCCTGTCTGAGCGTCGCT865300.9174403361608265No Hit
TAGCTTATCAGACTGATGTTGACT754020.7994549431087328No Hit
TGAGGTAGTAGGTTGTATAGTT745170.7900716691285833No Hit
AACCGGGCGGAAACACCA632710.6708351728791362No Hit
CATAAAGTAGAAAGCACTACT617020.6541997413821254No Hit
TGTAAACATCCTTGACTGGAAGCT611810.6486758026887268No Hit
TATTGCACTTGTCCCGGCCTGTA574600.6092236416942227No Hit
AGCAGCATTGTACAGGGCTATGA564840.5988755338923856No Hit
TCGTACGACTCTTAGCGG485250.5144896834878552No Hit
CAACGGAATCCCAAAAGCAGCTGT433480.4596001813463482No Hit
CTCCCACTGCTTCACTTGACTAG411290.4360730796944255No Hit
ACTGGACTTGGAGTCAGAAGGC410040.43474775851078856No Hit
CCCATAAAGTAGAAAGCACTAC398020.4220034700089358No Hit
TTCAAGTAATTCAGGATAGGTT395060.41886510944608357No Hit
TCCTGTCTGAGCGTCGCT394310.41806991673590144No Hit
TGAGGTAGTAGATTGTATAGTT387670.41102981060842203No Hit
TGTAAACATCCCCGACTGGAAGCT366900.3890082738211109No Hit
TTCTCACTACTGCACTTGACTAG358440.3800385000502562No Hit
CATTGCACTTGTCTCGGTCTGA355670.37710158830731677No Hit
TTAATGCTAATCGTGATAGGGGTT322310.34173141655841444No Hit
CTACGCCTGTCTGAGCGTCGCT314290.3332281558441999No Hit
CTTCTCACTACTGCACTTGACTAG280540.29744448388600286No Hit
CCCATAAAGTAGAAAGCACT279470.2963100089528097No Hit
TGTAAACATCCTACACTCTCAGCT278980.295790483048824No Hit
ACGCCTGTCTGAGCGTCGCT253740.2690295977088272No Hit
AACATTCAACGCTGTCGGTGAGTT248310.2632724024871083No Hit
TACGCCTGTCTGAGCGTCGCT235880.25009340863702273No Hit
TGATGTAGTAGGTTGTATAGTT230170.24403934147016926No Hit
CACCCGGCTGTGTGCACATGTGC217490.23059528338335625No Hit
CCCCCCACTGCTAAATTTGACTGG213680.2265557044156309No Hit
ACCGGGTGCTGTAGGCTT210340.22301444621295302No Hit
ATCCCGGACGAGCCCCCA210330.22300384364348394No Hit
TGTCAGTTTGTCAAATACCCCAA209470.22209202266914174No Hit
CCCCCCACTGCTAAATTTGACTGGCT197410.20930532388941264No Hit
CAACACCAGTCGATGGGCTGT194330.20603973249293125No Hit
GCGTTCGCGCTTTCCCCTG193400.20505369353230538No Hit
CTCCCACTGCTTCACTTGACTAGCCT188970.20035675525749613No Hit
CCCCCACTGCTAAATTTGACTGGCTT183980.1950660730924175No Hit
TGAGGTAGTAGTTTGTACAGTT171360.1816856304224191No Hit
CAACGGAATCCCAAAAGCAGCT166660.17670242277194426No Hit
TATTGCACTTGTCCCGGCCTGTT165310.17527107589361637No Hit
TGAGGTAGTAGTTTGTGCTGTT160860.17055293247986889No Hit
GGAATACCGGGTGCTGTAGGCTTT153360.16260100537804734No Hit
TTCACAGTGGCTAAGTTCCG152980.16219810773822171No Hit
ACCCCCCACTGCTAAATTTGACTG152120.16128628676387952No Hit
TAGCAGCACGTAAATATTGGC150700.15978072189926795No Hit
TTCAAGTAATCCAGGATAGGC143790.15245434639612304No Hit
TGTCAGTTTGTCAAATACCCCA139510.1479164466633502No Hit
TAGCAGCACGTAAATATTGGCGT138980.14735451048148812No Hit
TGTAAACATCCTACACTCAGCT138440.14678197173015697No Hit
AACGGAATCCCAAAAGCAGCTG137570.14585954818634567No Hit
ACCGGGTGCTGTAGGCTTT137500.145785330200062No Hit
ACTTGACTTGGAGTCAGAAGGC135800.14398289339031578No Hit
TTCAAGTAATCCAGGATAGGCTT131380.1392965576849756No Hit
ATCCCACCGCTGCCACCA129470.13727146691637837No Hit
ACCACAGGGTAGAACCACGGAC128470.13621120996946884No Hit
TACCTGGTTGATCCTGCC128320.13605217142743242No Hit
AGCTCGGTCTGAGGCCCCTCAGT128310.1360415688579633No Hit
GGAATACCGGGTGCTGTAGGCTT127710.1354054146898176No Hit
CACTAGATTGTGAGCTCCTGGA126480.13410129864511885No Hit
TGTAAACATCCCCGACTGGAAGC123990.1314612588473141No Hit
TGAGGTAGTAGGTTGTGTGGTT122940.13034798905305905No Hit
AGCAGCATTGTACAGGGCTATG122620.13000870683004803No Hit
TGATGTAGTAGATTGTATAGTT120230.12747469272693424No Hit
TAGCTTATCAGACTGATGTTGACA116950.12399704994107091No Hit
CAAAGTGCTGTTCGTGCAGGT116600.12362596000965258No Hit
TCCCACTGCTTCACTTGACTAG114950.12187653604725182No Hit
CAAAGAATTCTCCTTTTGGGCT113580.12042398402998576No Hit
TATTGCACTTGTCCCGGCCTGTAG112160.11891841916537421No Hit
CAAAGTGCTGTTCGTGCAGGTAG111280.11798539305209381No Hit
ACCGGGTGCTGTAGGCTTA108580.11512269929543806No Hit
TTCACAGTGGCTAAGTTCCGC106990.11343689074985187No Hit
AGCAGCATTGTACAGGGCTATGT106150.11254627491444787No Hit
GGGCTACGCCTGTCTGAGCGTCGCT105780.11215397984409134No Hit
AACATTCAACGCTGTCGGTGA102700.10888838844760995No Hit
TCGTTTCCCGGCCAATGCACCA101230.10732981073565291No Hit
TTCACAGTGGCTAAGTTCTGC99220.10519869427236474No Hit
TTCACCACCTTCTCCACCCAGC98560.10449892468740445No Hit
CAAAGAATTCTCCTTTTGGGCTT97520.10339625746261852No Hit
ACTGGACTTGGAGTCAGAAGG95750.10151960266658865No Hit
ACTGGACTTGGAGTCAGAAGGCA95380.1011273075962321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)