FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243812
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG109227115.078676807183841No Hit
CCCCCCACTGCTAAATTTGACTG6649709.179835147571472No Hit
CCCCCACTGCTAAATTTGACTG2637213.6406383821115185No Hit
TTCAAGTAATCCAGGATAGGCT2169542.9950252712245984No Hit
TAGCAGCACGTAAATATTGGCG1886882.604816359121413No Hit
TCGTACGACTCTTAGCGG1808832.4970692226689484No Hit
AAGCTGCCAGTTGAAGAACTGT1475272.0365934400285375No Hit
AACATTCAACGCTGTCGGTGAG1378981.9036661912263875No Hit
TAGCTTATCAGACTGATGTTGAC1208271.668002979646628No Hit
TGAGGTAGTAGATTGTATAGTT1150271.5879346399381982No Hit
TGAGAACTGAATTCCATGGGTT884171.2205866193103851No Hit
TGAGGTAGTAGGTTGTATAGTT870481.2016877301619644No Hit
TATTGCACTTGTCCCGGCCTGT835581.1535086774753405No Hit
TAGCTTATCAGACTGATGTTGA766681.0583930118561884No Hit
TATTGCACTTGTCCCGGCCTGTAA751921.0380169998890088No Hit
TAGCTTATCAGACTGATGTTGACT653130.9016385295477023No Hit
TTAATGCTAATCGTGATAGGGGTT643270.8880269117972692No Hit
TATTGCACTTGTCCCGGCCTGTAT630240.8700391451351857No Hit
CAACGGAATCCCAAAAGCAGCTGT535100.7386994582410477No Hit
AACATTCAACGCTGTCGGTGAGT495020.6833694745252914No Hit
TGTAAACATCCTTGACTGGAAGCT454900.6279842712649085No Hit
TATTGCACTTGTCCCGGCCTGTA430260.5939690317749826No Hit
TTCAAGTAATTCAGGATAGGTT418510.5777482905409472No Hit
TGAGAACTGAATTCCATAGGCTGT414950.5728337510691884No Hit
TGAGGTAGTAGTTTGTACAGTT413080.5702522373578994No Hit
AGCAGCATTGTACAGGGCTATGA392010.5411653422258889No Hit
TTCTCACTACTGCACTTGACTAG360380.49750048731248137No Hit
CATAAAGTAGAAAGCACTACT316160.4364552807278819No Hit
CATTGCACTTGTCTCGGTCTGA303780.41936483166597915No Hit
CCCATAAAGTAGAAAGCACTAC293420.4050629696077148No Hit
CACCCGGCTGTGTGCACATGTGC287080.3963106717844141No Hit
CAACGGAATCCCAAAAGCAGCT282800.3904021805093782No Hit
AACGGAATCCCAAAAGCAGCTG282230.3896153019984505No Hit
CCCCCACTGCTAAATTTGACTGGCTT271820.3752444155094031No Hit
ACTGGACTTGGAGTCAGAAGGC257420.3553653794438619No Hit
CTCCCACTGCTTCACTTGACTAG255420.3526044022125367No Hit
TGTAAACATCCCCGACTGGAAGCT254720.3516380601815729No Hit
CTTCTCACTACTGCACTTGACTAG224170.309464132973081No Hit
CCCATAAAGTAGAAAGCACT210070.2899992434922386No Hit
AACCGGGCGGAAACACCA200410.27666372346493806No Hit
TCGTTTCCCGGCCAATGCACCA186670.2576958098857342No Hit
ACCCCCCACTGCTAAATTTGACTG185280.2557769307099632No Hit
TAGCTTATCAGACTGATGTTGACA175960.24291077681198794No Hit
TAGCAGCACGTAAATATTGGC168960.23324735650234987No Hit
GGAATACCGGGTGCTGTAGGCTTT166880.2303759401817717No Hit
CAAAGAATTCTCCTTTTGGGCT156860.21654344425283265No Hit
CTCCCACTGCTTCACTTGACTAGCCT153200.21149085591950756No Hit
TTCACAGTGGCTAAGTTCCGC149690.20664534087853192No Hit
ACCGGGTGCTGTAGGCTT149620.20654870667543554No Hit
GCCTGTCTGAGCGTCGCT147030.20297324116086946No Hit
CCCCCCACTGCTAAATTTGACTGG145150.20037792256342382No Hit
TGTAAACATCCTACACTCTCAGCT141250.19499401696233973No Hit
TGAGAACTGAATTCCATGGGTTG141140.19484216321461684No Hit
CCCCCCACTGCTAAATTTGACTGGCT135310.18679391458530398No Hit
AGCTCGGTCTGAGGCCCCTCAGT131140.18103727705799103No Hit
ACCGGGTGCTGTAGGCTTT127080.17543249327840094No Hit
CAACACCAGTCGATGGGCTGT126090.17406580954889497No Hit
GGAATACCGGGTGCTGTAGGCTT125360.1730580528594613No Hit
TTAATGCTAATCGTGATAGGGGTTT119280.1646646820762328No Hit
TATTGCACTTGTCCCGGCCTGTT117330.16197272927569076No Hit
CCCATAAAGTAGAAAGCACTA115530.1594878497674981No Hit
TGAGGTAGTAGATTGTATAGT111970.1545733102957393No Hit
CACTAGATTGTGAGCTCCTGGA107270.1480850138021252No Hit
TTCACAGTGGCTAAGTTCTGC103720.14318427921652302No Hit
TGATGTAGTAGATTGTATAGTT102710.1417899857147038No Hit
TGTCAGTTTGTCAAATACCCCA101010.13944315506807742No Hit
TGAGGTAGTAGTTTGTGCTGTT100550.13880813030487263No Hit
CAAAGTGCTGTTCGTGCAGGTAG97630.1347771035471379No Hit
CACTAGATTGTGAGCTCCTGGAA92220.12730866013640332No Hit
CAAAGAATTCTCCTTTTGGGCTT91070.12572109822839134No Hit
TGAGGTAGTAAGTTGTATTGTT89820.12399548745881313No Hit
TTCAAGTAATCCAGGATAGGC89820.12399548745881313No Hit
AATGCACCCGGGCAAGGATTCT89730.1238712434834035No Hit
ATCACATTGCCAGGGATTTCC86660.11963314343331936No Hit
TATTGCACTTGTCCCGGCCTGTAG86000.11872202094698205No Hit
TTCAAGTAATCCAGGATAGGCTT85960.11866680140235557No Hit
TGGCTCAGTTCAGCAGGAAC84490.11663748313733156No Hit
ACTGGACTTGGAGTCAGAAGGCT84490.11663748313733156No Hit
TTAATGCTAATCGTGATAGGGGT84420.11654084893423518No Hit
AATCCTTGGAACCTAGGTGTGAGT82010.11321387137048836No Hit
GAATACCGGGTGCTGTAGGCTTT79540.10980406448980178No Hit
TGATGTAGTAGGTTGTATAGTT79260.10941752767741626No Hit
ATGCACCTGGGCAAGGATTCTGA76450.1055383546674044No Hit
TCAGTGCACTACAGAACTTTGT76100.1050551836519225No Hit
TGATAACTGAATTCCATGGGTT75640.10442015888871771No Hit
ACCGGGTGCTGTAGGCTTA74840.10331576799618764No Hit
TGTAAACATCCCCGACTGGA74480.10281879209454911No Hit
TCGTTTCCCGGCCAACGCACCA74210.10244606016832022No Hit
ACTGGACTTGGAGTCAGAAGGCA74000.10215615755903107No Hit
AGCAGCATTGTACAGGGCTATGT72750.10043054678945285No Hit
TCCCACTGCTTCACTTGACTAG72740.10041674190329622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)