FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13310154
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG193666014.550244873199814No Hit
CCCCCCACTGCTAAATTTGACTG9768507.339133717010336No Hit
TAGCAGCACGTAAATATTGGCG5215633.9185346766085503No Hit
TTCAAGTAATCCAGGATAGGCT5109383.8387084026225393No Hit
TGAGGTAGTAGATTGTATAGTT4703673.533896001503814No Hit
CCCCCACTGCTAAATTTGACTG3718662.793851971960655No Hit
AAGCTGCCAGTTGAAGAACTGT3358482.5232465379438884No Hit
TAGCTTATCAGACTGATGTTGAC3175062.38544197159552No Hit
AACATTCAACGCTGTCGGTGAG2676072.0105477367128888No Hit
TAGCTTATCAGACTGATGTTGA2205041.6566600206128343No Hit
TGAGGTAGTAGGTTGTATAGTT1915431.4390742586449412No Hit
TAGCTTATCAGACTGATGTTGACT1911771.4363244782892821No Hit
TTCAAGTAATTCAGGATAGGTT1908921.4341832558811867No Hit
TGAGGTAGTAGTTTGTACAGTT1745931.3117278733213755No Hit
TATTGCACTTGTCCCGGCCTGTAA1737251.305206536303036No Hit
TGTAAACATCCTTGACTGGAAGCT1358061.0203187731712196No Hit
TATTGCACTTGTCCCGGCCTGTAT1356751.0193345621696037No Hit
CATAAAGTAGAAAGCACTACT1291190.9700789337223296No Hit
TATTGCACTTGTCCCGGCCTGT1255720.9434301060678937No Hit
TCGTACGACTCTTAGCGG1240840.9322506711793117No Hit
CAACGGAATCCCAAAAGCAGCTGT979760.7360996724756153No Hit
TTCTCACTACTGCACTTGACTAG928010.6972195813812522No Hit
AACATTCAACGCTGTCGGTGAGT918180.6898342423385936No Hit
AGCAGCATTGTACAGGGCTATGA781380.5870555667500166No Hit
CATTGCACTTGTCTCGGTCTGA752960.5657034471577114No Hit
TATTGCACTTGTCCCGGCCTGTA736810.5535698535118376No Hit
TGAGAACTGAATTCCATGGGTT733370.5509853604999612No Hit
TTCACAGTGGCTAAGTTCCGC675530.507529815207247No Hit
CAAAGAATTCTCCTTTTGGGCT586370.4405433626087272No Hit
CTTCTCACTACTGCACTTGACTAG548070.411768338668358No Hit
TGTAAACATCCCCGACTGGAAGCT532830.40031843358085867No Hit
TGAGAACTGAATTCCATAGGCTGT518810.3897851219452457No Hit
TGTCAGTTTGTCAAATACCCCA514770.38674984526850703No Hit
CAACGGAATCCCAAAAGCAGCT506560.3805816221209762No Hit
GCCTGTCTGAGCGTCGCT479200.3600258870032608No Hit
TAGCAGCACGTAAATATTGGC470830.35373745487843344No Hit
AACGGAATCCCAAAAGCAGCTG467640.35134078839358285No Hit
TGAGGTAGTAGATTGTATAGT460800.346201854614154No Hit
TGATGTAGTAGATTGTATAGTT417080.3133547515678632No Hit
CCCCCACTGCTAAATTTGACTGGCTT416460.3128889417808389No Hit
TAGCTTATCAGACTGATGTTGACA401510.3016569154646896No Hit
ACTGGACTTGGAGTCAGAAGGC400640.30100327915063946No Hit
TCAGTGCACTACAGAACTTTGT377810.2838509607026335No Hit
CTCCCACTGCTTCACTTGACTAG370190.2781260081588838No Hit
CACCCGGCTGTGTGCACATGTGC365820.2748428004664709No Hit
TTCACAGTGGCTAAGTTCTGC349640.26268666763735415No Hit
TGTAAACATCCTACACTCTCAGCT345640.25968144320493963No Hit
CCCATAAAGTAGAAAGCACTAC338780.2545274833033487No Hit
CAAAGAATTCTCCTTTTGGGCTT316690.23793113137533947No Hit
CCCATAAAGTAGAAAGCACT304690.22891545807809588No Hit
TCAGTGCACTACAGAACTTTGTC293600.22058347333922657No Hit
TGAGGTAGTAGATTGTATAGTTA288460.21672175994357393No Hit
TGAGGTAGTAAGTTGTATTGTT285870.2147758771235855No Hit
TGAGGTAGTAGTTTGTGCTGTT277990.20885558499172888No Hit
TCGTTTCCCGGCCAATGCACCA273280.20531693322256078No Hit
TGTAAACATCCCCGACTGGA272350.2046182185420244No Hit
CAACACCAGTCGATGGGCTGT245250.18425782301241594No Hit
TGTCAGTTTGTCAAATACCCCAA242460.1821616789708068No Hit
TTATAAAGCAATGAGACTGATT239420.17987770840217177No Hit
TGTCAGTTTGTCAAATACCCC238790.17940438555406646No Hit
CCCCCCACTGCTAAATTTGACTGG229760.17262009139789067No Hit
ACCCCCCACTGCTAAATTTGACTG225820.16965994533196235No Hit
CAAAGAATTCTCCTTTTGGGC224460.16863816902494141No Hit
TTCAAGTAATCCAGGATAGGC219720.1650769780725302No Hit
CTCCCACTGCTTCACTTGACTAGCCT219120.16462619440766801No Hit
TTCACAGTGGCTAAGTTCCG217110.1631160691303797No Hit
TAGCAGCACATCATGGTTTACA212230.15944969532283398No Hit
TGGCTCAGTTCAGCAGGAAC205270.1542206048104327No Hit
ATCACATTGCCAGGGATTTCC201830.1516361117985562No Hit
CCCCCCACTGCTAAATTTGACTGGCT198130.14885627919857275No Hit
TTCACAGTGGCTAAGTTCTG197700.14853321757208818No Hit
TTCAAGTAATCCAGGATAGGCTT197330.14825523431208984No Hit
TTCAAGTAATTCAGGATAGGTTA197300.14823269512884674No Hit
TGAGGTAGTAGTTTGTGCTGTTA192790.14484430458129935No Hit
TATTGCACTTGTCCCGGCCTGTT187620.14096005200240358No Hit
TGTAAACATCCCCGACTGGAAGC184480.13860095082295817No Hit
CAAAGTGCTGTTCGTGCAGGTAG182810.1373462696224251No Hit
TATTGCACTTGTCCCGGCCTGTAG172850.12986326078571292No Hit
AATCCTTGGAACCTAGGTGTGAGT172060.12926972896031105No Hit
AACCGGGCGGAAACACCA170260.12791737796572453No Hit
CACTAGATTGTGAGCTCCTGGAA169210.1271285065522157No Hit
AGCTCGGTCTGAGGCCCCTCAGT167650.12595646902357402No Hit
TGATGTAGTAGGTTGTATAGTT165450.12430359558574604No Hit
ATGCACCTGGGCAAGGATTCTGA159700.11998358546415014No Hit
CACTAGATTGTGAGCTCCTGGA155270.11665529940525106No Hit
TGATGTAGTAGTTTGTACAGTT153030.11497237372309892No Hit
TGTCAGTTTGTCAAATACC152060.1142436067982384No Hit
TAGCAGCACGTAAATATTGGCGT150480.11305654314743466No Hit
TGTCAGTTTGTCAAATACCC149910.1126282986658156No Hit
CCCATAAAGTAGAAAGCACTA144970.10891684649178364No Hit
TATTGCACTTGTCCCGGCCTGTAAT142130.10678313714476934No Hit
AGCAGCATTGTACAGGGCTATGT141870.10658779755666238No Hit
TGAGGTAGTAGTTTGTACAGTTA140700.10570876941018113No Hit
AGCTACATTGTCTGCTGGGTTT136930.10287634538263042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)