FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13002079
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9158697.044019652549411No Hit
CAACGGAATCCCAAAAGCAGCTG8409866.468088680279516No Hit
AAGCTGCCAGTTGAAGAACTGT5266254.050313799816168No Hit
AACATTCAACGCTGTCGGTGAGT4665303.588118484743863No Hit
TTCAAGTAATCCAGGATAGGCT4322153.324199153073905No Hit
TAGCAGCACGTAAATATTGGCG4162783.201626447585805No Hit
GCCTGTCTGAGCGTCGCT3123902.4026157662939904No Hit
CCCCCACTGCTAAATTTGACTG3051062.3465939562434595No Hit
AACATTCAACGCTGTCGGTGAG2744162.110554781277671No Hit
TAGCTTATCAGACTGATGTTGAC2009071.5451913497833691No Hit
CGCCTGTCTGAGCGTCGCT2001701.5395230255099974No Hit
TATTGCACTTGTCCCGGCCTGTAA1824321.403098689063495No Hit
TGAGGTAGTAGGTTGTATAGTT1609021.2375097859350033No Hit
TATTGCACTTGTCCCGGCCTGTAT1536271.1815571955838755No Hit
TATTGCACTTGTCCCGGCCTGT1428141.0983935722894778No Hit
TGTAAACATCCTTGACTGGAAGCT1427751.0980936202587295No Hit
TAGCTTATCAGACTGATGTTGACT1343161.0330347938971913No Hit
AGCAGCATTGTACAGGGCTATGA1149010.8837125201285119No Hit
TAGCTTATCAGACTGATGTTGA1039580.7995490567316197No Hit
CATAAAGTAGAAAGCACTACT999740.7689078031290226No Hit
AACCGGGCGGAAACACCA980480.7540947874566829No Hit
TATTGCACTTGTCCCGGCCTGTA928530.7141396387454652No Hit
TGAGGTAGTAGATTGTATAGTT872670.671177278649053No Hit
ACTGGACTTGGAGTCAGAAGGC847340.6516957788058356No Hit
TTCAAGTAATTCAGGATAGGTT751470.577961416785731No Hit
TGTAAACATCCCCGACTGGAAGCT717240.5516348577792828No Hit
TACGCCTGTCTGAGCGTCGCT706440.543328493850868No Hit
TCCTGTCTGAGCGTCGCT656800.5051499840910058No Hit
TTAATGCTAATCGTGATAGGGGTT546140.4200405181355997No Hit
CTACGCCTGTCTGAGCGTCGCT530280.4078424688851683No Hit
CCCATAAAGTAGAAAGCACTAC508190.3908528782204754No Hit
GCGTTCGCGCTTTCCCCTG507750.3905144708011696No Hit
CATTGCACTTGTCTCGGTCTGA498700.3835540454722664No Hit
CAACGGAATCCCAAAAGCAGCTGT477320.3671105213250896No Hit
TGTAAACATCCTACACTCTCAGCT473930.36450324598089273No Hit
ACGCCTGTCTGAGCGTCGCT453560.348836520682577No Hit
TCGTACGACTCTTAGCGG447670.34430647591050634No Hit
CCCATAAAGTAGAAAGCACT430610.3311854973346955No Hit
AACATTCAACGCTGTCGGTGAGTT413340.3179030061269432No Hit
TGAGGTAGTAGTTTGTACAGTT391850.30137488012494No Hit
GGAATACCGGGTGCTGTAGGCTTT381090.29309928050737116No Hit
CAACACCAGTCGATGGGCTGT348340.2679110010022243No Hit
CAACGGAATCCCAAAAGCAGCT301180.2316398785148129No Hit
GGAATACCGGGTGCTGTAGGCTT297430.22875572437300218No Hit
CTCCCACTGCTTCACTTGACTAG293560.22577927729865357No Hit
TTCTCACTACTGCACTTGACTAG292020.22459485133108328No Hit
TGAGGTAGTAGTTTGTGCTGTT290540.223456571829782No Hit
CACCCGGCTGTGTGCACATGTGC276150.21238911100294036No Hit
CTTCTCACTACTGCACTTGACTAG271220.20859740969117324No Hit
TTCAAGTAATCCAGGATAGGCTT271120.2085204989140583No Hit
AGCTCGGTCTGAGGCCCCTCAGT270470.2080205788628111No Hit
TAGCAGCACGTAAATATTGGC265050.2038520147431807No Hit
AACGGAATCCCAAAAGCAGCTG248100.19081563802219628No Hit
ATCCCGGACGAGCCCCCA246410.1895158458889536No Hit
TTCACAGTGGCTAAGTTCCG243530.18730081550804298No Hit
TGTCAGTTTGTCAAATACCCCAA241900.18604716984106925No Hit
AGCAGCATTGTACAGGGCTATGT240360.184862743873499No Hit
CCTCGACACAAGGGTTTGT240350.1848550527957875No Hit
TTATAAAGCAATGAGACTGATT238590.18350142311856435No Hit
CCAAGTCCTTCTGATCGAGGCCC226370.1741029261551172No Hit
TATTGCACTTGTCCCGGCCTGTT219050.16847305727030268No Hit
TGTAAACATCCTACACTCAGCT216610.16659643430869786No Hit
ACCACAGGGTAGAACCACGGAC211620.16275858653066175No Hit
ACTGGACTTGGAGTCAGAAGGCA211440.16262014713185483No Hit
CAAAGAATTCTCCTTTTGGGCT210450.1618587304384168No Hit
CAAAGAATTCTCCTTTTGGGCTT209660.16125113529920868No Hit
TAGCAGCACGTAAATATTGGCGT207450.15955140712496824No Hit
TATTGCACTTGTCCCGGCCTGTAG204660.15740559644346108No Hit
TTCAAGTAATCCAGGATAGGC204570.15733637674405762No Hit
ACTGGACTTGGAGTCAGAAGG193940.1491607611367382No Hit
TGAGGTAGTAGGTTGTGTGGTT193450.14878389832887495No Hit
TGTCAGTTTGTCAAATACCCCA191380.14719184524259543No Hit
CCCCCCACTGCTAAATTTGACTGG191060.1469457307558276No Hit
TGTAAACATCCCCGACTGGAAGC188640.14508448994964576No Hit
TAGCTTATCAGACTGATGTTGACA187730.14438460187789967No Hit
AGCAGCATTGTACAGGGCTATG184710.14206189640902814No Hit
CAAGTCCTTCTGATCGAGGCCC176130.13546295173256523No Hit
CACTAGATTGTGAGCTCCTGGA174440.13416315959932254No Hit
TTCACAGTGGCTAAGTTCCGC170730.13130976976835781No Hit
CTCGACACAAGGGTTTGT165780.12750268630116768No Hit
TACCTGGTTGATCCTGCC164820.12676434284086413No Hit
CAAAGTGCTGTTCGTGCAGGTAG162640.1250876878997582No Hit
TCGTTTCCCGGCCAATGCACCA161380.12411861210810979No Hit
AGAGGTAGTAGGTTGCATAGTT159900.12298033260680849No Hit
TTCACAGTGGCTAAGTTCTGC158550.12194203711575664No Hit
ACTGGACTTGGAGTCAGAAGGCT158250.12171130478441179No Hit
CAAAGTGCTGTTCGTGCAGGT157810.12137289736510601No Hit
TGAGGTAGTAGGTTGTATAGT156730.1205422609722645No Hit
CCCCCCACTGCTAAATTTGACTGGCT150470.11572764632486851No Hit
TCAGTGCACTACAGAACTTTGT149720.11515081549650637No Hit
CACTAGATTGTGAGCTCCTGGAA149420.11492008316516152No Hit
CTGCGTTCGCGCTTTCCCCTG147360.11333572115659349No Hit
TTCACAGTGGCTAAGTTCTG147340.1133203390011705No Hit
CCCCCACTGCTAAATTTGACTGGCTT140320.10792120244770087No Hit
GGGCTACGCCTGTCTGAGCGTCGCT137690.10589844900957762No Hit
AATGACACGATCACTCCCGTTG136860.10526008955952353No Hit
ACCGGGTGCTGTAGGCTT132220.10169142950138975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)