FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629949
Sequences flagged as poor quality0
Sequence length18-26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG86144511.29031137691746No Hit
TGAGGTAGTAGATTGTATAGTT4197605.501478450249143No Hit
TTCAAGTAATCCAGGATAGGCT3265634.280015502069542No Hit
CCCCCCACTGCTAAATTTGACTG2631763.4492497918400242No Hit
TAGCAGCACGTAAATATTGGCG2327373.0503087242129667No Hit
TAGCTTATCAGACTGATGTTGAC2102132.7551036055417932No Hit
TGAGGTAGTAGGTTGTATAGTT1910232.503594716032833No Hit
TAGCTTATCAGACTGATGTTGA1889732.476726908659547No Hit
TCGTACGACTCTTAGCGG1578062.0682444928530974No Hit
CCCCCACTGCTAAATTTGACTG1559352.0437227037821617No Hit
AAGCTGCCAGTTGAAGAACTGT1554652.0375627674575547No Hit
AACATTCAACGCTGTCGGTGAG1377021.8047564931299016No Hit
TGAGAACTGAATTCCATGGGTT1297821.7009550129365214No Hit
TGAGGTAGTAGTTTGTACAGTT1276501.6730124932683037No Hit
CATAAAGTAGAAAGCACTACT1178901.5450955176764616No Hit
TTCAAGTAATTCAGGATAGGTT1165531.5275724647700792No Hit
TAGCTTATCAGACTGATGTTGACT964721.2643859087393639No Hit
TATTGCACTTGTCCCGGCCTGT956701.2538746982450342No Hit
TGTAAACATCCTTGACTGGAAGCT673580.8828106190486987No Hit
TATTGCACTTGTCCCGGCCTGTAA610870.8006213409814404No Hit
CATTGCACTTGTCTCGGTCTGA516690.6771867020343124No Hit
TTAATGCTAATCGTGATAGGGGTT515550.6756925898194077No Hit
TATTGCACTTGTCCCGGCCTGTAT504960.6618130737177929No Hit
TGAGGTAGTAGATTGTATAGT501160.6568326996681105No Hit
TGAGAACTGAATTCCATAGGCTGT500980.6565967872131255No Hit
TGATGTAGTAGATTGTATAGTT491640.6443555520489063No Hit
TTCACAGTGGCTAAGTTCCGC486590.6377368970618283No Hit
TATTGCACTTGTCCCGGCCTGTA427730.5605935242817481No Hit
AGCAGCATTGTACAGGGCTATGA416320.5456392958852019No Hit
AACATTCAACGCTGTCGGTGAGT412240.5402919469055429No Hit
GCCTGTCTGAGCGTCGCT361490.4737777408472848No Hit
TGAGGTAGTAAGTTGTATTGTT361440.473712209609789No Hit
CAACGGAATCCCAAAAGCAGCTGT357720.46883668554009994No Hit
CAACGGAATCCCAAAAGCAGCT303100.3972503616996654No Hit
TTCTCACTACTGCACTTGACTAG245570.32185011983697404No Hit
TTATAAAGCAATGAGACTGATT239470.3138553088624839No Hit
CAACACCAGTCGATGGGCTGT239080.3133441652100165No Hit
TCAGTGCACTACAGAACTTTGT233050.3054410979680205No Hit
TAGCAGCACGTAAATATTGGC231040.3028067422206885No Hit
AACGGAATCCCAAAAGCAGCTG230220.30173202992575704No Hit
TGTAAACATCCCCGACTGGAAGCT223930.2934882002487828No Hit
TGATGTAGTAGGTTGTATAGTT219660.2878918325666397No Hit
TCGTTTCCCGGCCAATGCACCA212070.2779441907147741No Hit
TGAGGTAGTAGTTTGTGCTGTT211930.2777607032497858No Hit
TTCACAGTGGCTAAGTTCTGC208810.2736715540300466No Hit
TAGCTTATCAGACTGATGTTGACA207930.27251820425012013No Hit
CACCCGGCTGTGTGCACATGTGC205610.26947755483031405No Hit
AACCGGGCGGAAACACCA200450.2627147311207454No Hit
ACTGGACTTGGAGTCAGAAGGC199320.2612337251533398No Hit
TGTCAGTTTGTCAAATACCCCA193770.2539597577913037No Hit
TGAGGTAGTAGATTGTATAGTTA181200.2374852046848544No Hit
CAAAGAATTCTCCTTTTGGGCT179310.23500812390751233No Hit
TGTAAACATCCCCGACTGGA179180.23483774269002322No Hit
TGTAAACATCCTACACTCTCAGCT176040.2307223809752857No Hit
TGGCTCAGTTCAGCAGGAAC166930.21878258950354715No Hit
TTCACAGTGGCTAAGTTCTG161020.21103679723154115No Hit
TCAGTGCACTACAGAACTTTGTC152850.200328993024724No Hit
TGATGTAGTAGTTTGTACAGTT148520.19465398785758595No Hit
TGATAACTGAATTCCATGGGTT144050.18879549522545958No Hit
TTCAAGTAATCCAGGATAGGC141940.18603007700313592No Hit
TGAGAACTGAATTCCATGGGTTG139990.18347435874079893No Hit
TTCACAGTGGCTAAGTTCCG135900.1781139035136408No Hit
TGAGGTAGTAGGTTGTATAGT132360.17347429189893668No Hit
TAGCTTATCAGACTGATGTTG126750.16612168705190558No Hit
TTCAAGTAATCCAGGATAGGCTT120840.15837589477989958No Hit
CCCATAAAGTAGAAAGCACTAC120520.15795649485992633No Hit
AGCTACATTGTCTGCTGGGTTT120510.15794338861242718No Hit
CTTCTCACTACTGCACTTGACTAG114660.15027623382541613No Hit
TATTGCACTTGTCCCGGCCTGTT112900.14796953426556325No Hit
CAAAGAATTCTCCTTTTGGGCTT112740.14775983430557663No Hit
TGTCAGTTTGTCAAATACC112530.1474846031080942No Hit
TGAGGTAGTAGTTTGTGCTGTTA109160.14306779770087585No Hit
CAAAGTGCTGTTCGTGCAGGTAG106810.13998782953857228No Hit
CTCCCACTGCTTCACTTGACTAG106310.13933251716361408No Hit
TGTCAGTTTGTCAAATACCCCAA103820.1360690615363222No Hit
CCCATAAAGTAGAAAGCACT103250.13532200542886982No Hit
TAGCAGCACATCATGGTTTACA98330.1288737316592811No Hit
TAGCAGCACGTAAATATTGGCGT97930.12834948175931452No Hit
CCCCCACTGCTAAATTTGACTGGCTT97670.12800871932433625No Hit
ATCACATTGCCAGGGATTTCC94500.12385403886710121No Hit
TTCAAGTAATTCAGGATAGGTTA94150.12339532020463047No Hit
TGAGGTAGTAGTTTGTACAGT94040.12325115148213966No Hit
TCGACCGGACCTCGACCGGCT93120.12204537671221655No Hit
TGTCAGTTTGTCAAATACCCC90310.11836252116495143No Hit
TCGTTTCCCGGCCAACGCACCA85510.11207152236535264No Hit
AGTGTTTCCTACTTTATGGATG85260.11174386617787355No Hit
AGCAGCATTGTACAGGGCTATGT84880.1112458287729053No Hit
CAAAGAATTCTCCTTTTGGGC84250.11042013518045796No Hit
AATGCACCCGGGCAAGGATTCT83370.10926678540053152No Hit
TAGCAGCACATAATGGTTTGT82390.10798237314561343No Hit
TTCAAGTAATTCAGGATAGGT81660.10702561707817443No Hit
AATCCTTGGAACCTAGGTGTGAGT80190.10509899869579731No Hit
TGTCAGTTTGTCAAATACCC80080.10495482997330652No Hit
CACCCGTAGAACCGACCTTGCG79410.10407671139086251No Hit
CACTAGATTGTGAGCTCCTGGA78660.1030937428284252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)