FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11894072
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9381817.887803268720754No Hit
CCCCCCACTGCTAAATTTGACTG7838336.590114806770969No Hit
TTCAAGTAATCCAGGATAGGCT4418223.714640368748398No Hit
AACATTCAACGCTGTCGGTGAGT4116753.461177971682028No Hit
TAGCAGCACGTAAATATTGGCG4100423.4474484432244905No Hit
AAGCTGCCAGTTGAAGAACTGT4090913.4394528635777557No Hit
AACATTCAACGCTGTCGGTGAG3044902.5600147703830953No Hit
CCCCCACTGCTAAATTTGACTG3025432.5436452713587068No Hit
TGAGGTAGTAGGTTGTATAGTT1909111.6050936970954943No Hit
TATTGCACTTGTCCCGGCCTGT1816591.5273070484187417No Hit
TATTGCACTTGTCCCGGCCTGTAA1803471.5162763433750863No Hit
TATTGCACTTGTCCCGGCCTGTAT1665481.400260566776458No Hit
TCGTACGACTCTTAGCGG1658381.3942912065775286No Hit
CATAAAGTAGAAAGCACTACT1514441.273272937981206No Hit
TGAGGTAGTAGATTGTATAGTT1295371.0890887494207198No Hit
ATCCCGGACGAGCCCCCA1267101.0653206067694898No Hit
TAGCTTATCAGACTGATGTTGA1213411.0201804730961777No Hit
TAGCTTATCAGACTGATGTTGAC1207551.0152536490446669No Hit
TATTGCACTTGTCCCGGCCTGTA1136430.955459156460462No Hit
TAGCTTATCAGACTGATGTTGACT1088580.9152290317395086No Hit
TTCAAGTAATTCAGGATAGGTT921040.7743689461439278No Hit
TGTAAACATCCTTGACTGGAAGCT871110.7323900511111754No Hit
TTAATGCTAATCGTGATAGGGGTT767580.6453466903512943No Hit
CCCATAAAGTAGAAAGCACT755930.6355518950953046No Hit
GCCTGTCTGAGCGTCGCT749800.630398067205243No Hit
CGCCTGTCTGAGCGTCGCT749460.6301122105196606No Hit
AGCAGCATTGTACAGGGCTATGA729300.6131625905745316No Hit
CATTGCACTTGTCTCGGTCTGA692370.582113509990523No Hit
AACCGGGCGGAAACACCA671910.5649116635581154No Hit
TGAGGTAGTAGTTTGTACAGTT584560.4914717180121324No Hit
CCCATAAAGTAGAAAGCACTAC584170.49114382357867015No Hit
ACTGGACTTGGAGTCAGAAGGC542820.4563786060820886No Hit
TGTAAACATCCCCGACTGGAAGCT505160.4247157743790352No Hit
CAACGGAATCCCAAAAGCAGCTGT486560.40907773216775545No Hit
CAACACCAGTCGATGGGCTGT475950.4001573220676653No Hit
TGAGAACTGAATTCCATGGGTT440960.3707393061013924No Hit
TTCACAGTGGCTAAGTTCCG427080.3590696272899643No Hit
CAACGGAATCCCAAAAGCAGCT399180.33561256397304473No Hit
TGAGGTAGTAGTTTGTGCTGTT357910.30091460687307087No Hit
TGTAAACATCCTACACTCTCAGCT327550.27538928636046595No Hit
AACATTCAACGCTGTCGGTGAGTT310760.2612730106224344No Hit
TGTCAGTTTGTCAAATACCCCAA307860.25883482124540697No Hit
TTCTCACTACTGCACTTGACTAG297660.250259120677931No Hit
TATTGCACTTGTCCCGGCCTGTT297590.25020026783089927No Hit
CACCCGGCTGTGTGCACATGTGC293340.24662705926111764No Hit
AGCTCGGTCTGAGGCCCCTCAGT280090.23548705607297485No Hit
AACGGAATCCCAAAAGCAGCTG279610.23508349369332893No Hit
CTCCCACTGCTTCACTTGACTAG265040.22283369396115982No Hit
TTCACAGTGGCTAAGTTCTG253330.21298845340771436No Hit
TTCAAGTAATCCAGGATAGGC246710.20742265558843093No Hit
ATCCCACCGCTGCCACCA239910.2017055218767803No Hit
CAAAGAATTCTCCTTTTGGGCT238870.20083113672088077No Hit
CTACGCCTGTCTGAGCGTCGCT233930.19667780723035813No Hit
CACTAGATTGTGAGCTCCTGGA231870.194945852017711No Hit
TATTGCACTTGTCCCGGCCTGTAG228750.1923226965500125No Hit
TAGCAGCACGTAAATATTGGC227310.19111200941107467No Hit
CCCATAAAGTAGAAAGCACTA226490.19042259034584624No Hit
TACGCCTGTCTGAGCGTCGCT225530.18961546558655437No Hit
TCAGTGCACTACAGAACTTTGT224330.18860655963743955No Hit
CTTCTCACTACTGCACTTGACTAG220740.18558824933967105No Hit
ACCGGGTGCTGTAGGCTT215740.18138447455169265No Hit
ACCGGGTGCTGTAGGCTTA211890.17814756796494927No Hit
TGAGGTAGTAGGTTGTATAGT209260.17593638242647264No Hit
AGCTACATTGTCTGCTGGGTTT208070.17493588402693375No Hit
TGTCAGTTTGTCAAATACCCCA205300.17260699279439373No Hit
TTATAAAGCAATGAGACTGATT196890.16553624360101402No Hit
TATTGCACTTGTCCCGGCCTGA192770.1620723331757198No Hit
TTCAAGTAATCCAGGATAGGCTT186790.1570446185292976No Hit
ACTGGACTTGGAGTCAGAAGG185100.1556237426509609No Hit
CACTAGATTGTGAGCTCCTGGAA184880.15543877656028987No Hit
ACGCCTGTCTGAGCGTCGCT181040.15221027752312244No Hit
TATTGCACTTGTCCCGGCCTG179280.15073054879775402No Hit
TCGTTTCCCGGCCAATGCACCA179140.15061284310369066No Hit
CAAAGTGCTGTTCGTGCAGGT173700.14603913613437014No Hit
ACCACAGGGTAGAACCACGGAC169720.1426929314031393No Hit
AACATTCAACGCTGTCGGTGA168890.1419951047883349No Hit
TTCACAGTGGCTAAGTTCTGC166760.1402042967286561No Hit
TCGTTTCCCGGGCGGCGCACCA166320.139834364547314No Hit
AGCAGCATTGTACAGGGCTATG165060.13877501330074343No Hit
TCCTGTCTGAGCGTCGCT162910.13696739014191273No Hit
AGAGGTAGTAGGTTGCATAGTT159000.1336800382577136No Hit
TGAGGTAGTAGGTTGTGTGGTT157270.13222553218107308No Hit
ACTGGACTTGGAGTCAGAAGGCA156340.13144363007050908No Hit
AATGACACGATCACTCCCGTTG156210.13133433192602165No Hit
TGTAAACATCCCCGACTGGAAGC155880.13105688279001507No Hit
CAAAGAATTCTCCTTTTGGGCTT154080.12954352386634282No Hit
TTCACAGTGGCTAAGTTCCGC151700.1275425270672651No Hit
TGAGGTAGTAGATTGTATAGT151030.12697922124567598No Hit
ACCGGGTGCTGTAGGCTTT148990.12526408113218082No Hit
AGCAGCATTGTACAGGGCTATGT148500.12485211120295892No Hit
ACTGGACTTGGAGTCAGAAGGCT143820.12091737800141113No Hit
TATTGCACTTGTCCCGGCCTGTTT141150.11867256226463065No Hit
TGTAAACATCCTACACTCAGCT140430.11806721869516176No Hit
TGTCAGTTTGTCAAATACC140010.11771410161297156No Hit
CCCCCCACTGCTAAATTTGACTGG137470.11557858402067853No Hit
TGATGTAGTAGGTTGTATAGTT136880.11508253859569709No Hit
TTCACCACCTTCTCCACCCAGC134460.11304791159831554No Hit
TGAGAACTGAATTCCATAGGCTGT134210.1128377228589166No Hit
CAAAGTGCTGTTCGTGCAGGTAG133090.11189607730640945No Hit
AGCTACATCTGGCTACTGGGTCTC131770.11078628076238313No Hit
TGAGGTAGTAAGTTGTATTGTT130770.10994552580478746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)