FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6856517
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6348429.258957572773465No Hit
CCCCCCACTGCTAAATTTGACTG5766498.410232192234044No Hit
AACATTCAACGCTGTCGGTGAGT2333563.4034189662185623No Hit
TAGCAGCACGTAAATATTGGCG2333433.403229365580221No Hit
TTCAAGTAATCCAGGATAGGCT2317473.3799522410576683No Hit
CCCCCACTGCTAAATTTGACTG1821932.6572237770284826No Hit
AAGCTGCCAGTTGAAGAACTGT1661402.423096158005588No Hit
AACATTCAACGCTGTCGGTGAG1542052.249028187343516No Hit
TAGCTTATCAGACTGATGTTGAC1223111.7838648981691432No Hit
TAGCTTATCAGACTGATGTTGACT1129631.6475274545370486No Hit
TATTGCACTTGTCCCGGCCTGTAA1110061.6189852661343946No Hit
TGTAAACATCCTTGACTGGAAGCT858061.2514517210414557No Hit
TGAGGTAGTAGGTTGTATAGTT848671.2377567210873974No Hit
TATTGCACTTGTCCCGGCCTGTAT783631.1428980632586487No Hit
TAGCTTATCAGACTGATGTTGA683610.9970222490515228No Hit
CATAAAGTAGAAAGCACTACT553810.8077133039996838No Hit
AGCAGCATTGTACAGGGCTATGA538100.7848007960893265No Hit
TGAGGTAGTAGATTGTATAGTT476370.6947696622060443No Hit
GCCTGTCTGAGCGTCGCT454000.6621437677468021No Hit
TATTGCACTTGTCCCGGCCTGT443750.6471944866467917No Hit
TGTAAACATCCCCGACTGGAAGCT429450.6263384164292162No Hit
CCCCCACTGCTAAATTTGACTGGCTT410180.5982337679611966No Hit
CCCATAAAGTAGAAAGCACTAC408450.5957106210048047No Hit
TATTGCACTTGTCCCGGCCTGTA389200.5676351418657607No Hit
TTCAAGTAATTCAGGATAGGTT383530.5593656371011696No Hit
ACTGGACTTGGAGTCAGAAGGC369700.5391950461145214No Hit
CAACGGAATCCCAAAAGCAGCTGT363540.5302108927900274No Hit
TGTCAGTTTGTCAAATACCCCAA314660.4589210527735876No Hit
CAAAGAATTCTCCTTTTGGGCT287910.4199070752686824No Hit
TGTAAACATCCTACACTCTCAGCT262660.3830807974369494No Hit
CCCATAAAGTAGAAAGCACT228740.3336096154942808No Hit
CATTGCACTTGTCTCGGTCTGA218710.31898119701300237No Hit
TAGCTTATCAGACTGATGTTGACA217380.317041436636123No Hit
TGAGGTAGTAGTTTGTACAGTT211550.30853857723972683No Hit
AACATTCAACGCTGTCGGTGAGTT207660.30286514275396675No Hit
TCCTGTCTGAGCGTCGCT195480.2851010214078081No Hit
CCCCCCACTGCTAAATTTGACTGGCT190300.27754616520311987No Hit
TTCTCACTACTGCACTTGACTAG189470.27633563805063127No Hit
CGCCTGTCTGAGCGTCGCT188790.2753438808654598No Hit
TGTCAGTTTGTCAAATACCCCA184880.26964127705072416No Hit
CTTCTCACTACTGCACTTGACTAG183430.26752650069999095No Hit
CAAAGAATTCTCCTTTTGGGCTT175420.25584418444525114No Hit
TAGCAGCACGTAAATATTGGC171700.250418689255784No Hit
CAACGGAATCCCAAAAGCAGCT165090.2407782260293382No Hit
AACGGAATCCCAAAAGCAGCTG144420.21063172453302456No Hit
ACCCCCCACTGCTAAATTTGACTG135150.19711174055282005No Hit
AACCGGGCGGAAACACCA132510.19326138912803686No Hit
ACTGGACTTGGAGTCAGAAGGCA132300.19295511117379274No Hit
TGTAAACATCCCCGACTGGAAGC131260.19143830606706No Hit
CTCCCACTGCTTCACTTGACTAG128860.1879379865899844No Hit
CCCCCCACTGCTAAATTTGACTGG127760.18633367349632474No Hit
TGAGGTAGTAGTTTGTGCTGTT123480.1800914370955399No Hit
TTCAAGTAATCCAGGATAGGCTT121450.17713075020451347No Hit
TTCACAGTGGCTAAGTTCCG115680.16871539879504419No Hit
TATTGCACTTGTCCCGGCCTGTAG114950.1676507182874337No Hit
CACCCGGCTGTGTGCACATGTGC114120.16644019113494504No Hit
TATTGCACTTGTCCCGGCCTGTAAA111580.16273568635504004No Hit
CAACACCAGTCGATGGGCTGT111350.16240023907182027No Hit
CTCCCACTGCTTCACTTGACTAGCCT110160.1606646639977703No Hit
CACTAGATTGTGAGCTCCTGGAA107120.15623092599347455No Hit
ATCACATTGCCAGGGATTTCCA106630.1555162774335716No Hit
TTCAAGTAATCCAGGATAGGC105190.15341608574732624No Hit
TCGTTTCCCGGCCAATGCACCA101400.14788849790644434No Hit
TAGCAGCACGTAAATATTGGCGT100250.1462112614903456No Hit
ATCACATTGCCAGGGATTTCC99560.14520491964068638No Hit
AGCAGCATTGTACAGGGCTATGT98810.14411106980410024No Hit
ACTGGACTTGGAGTCAGAAGGCT98470.14361519121151453No Hit
TCGTACGACTCTTAGCGG94080.13721252350136373No Hit
TTCACAGTGGCTAAGTTCCGC93920.13697916886955872No Hit
TATTGCACTTGTCCCGGCCTGTT91900.13403306664302006No Hit
TATTGCACTTGTCCCGGCCTGTAAT91820.13391638932711752No Hit
TCAGTGCACTACAGAACTTTGT91080.13283712415501922No Hit
TGTAAACATCCTACACTCAGCT90040.13132031904828648No Hit
ATGCACCTGGGCAAGGATTCTGA89910.1311307184099449No Hit
TTCACAGTGGCTAAGTTCTGC89420.13041606985004195No Hit
TTATAAAGCAATGAGACTGATT89330.1302848078696516No Hit
TAGCAGCACATCATGGTTTACA88710.12938055867140708No Hit
CACTAGATTGTGAGCTCCTGGA87530.12765956826184488No Hit
TTAATGCTAATCGTGATAGGGGTT86380.12598233184574617No Hit
CCCCCACTGCTAAATTTGACTGGCTA85610.12485931268018441No Hit
CAAAGTGCTGTTCGTGCAGGTAG84930.123867555495013No Hit
CTACGCCTGTCTGAGCGTCGCT84870.1237800475080861No Hit
CAGTGCAATAGTATTGTCAAAGC83560.12186945646018234No Hit
CTACGGGGATGATTTTACGAACTGAA83290.12147567051901133No Hit
ACTGGACTTGGAGTCAGAAGG82920.12093603793296218No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC82490.12030889735998612No Hit
CCCATAAAGTAGAAAGCACTA82140.1197984341029126No Hit
TACGCCTGTCTGAGCGTCGCT80110.11683774721188615No Hit
TTCACAGTGGCTAAGTTCTG79340.11571472804632439No Hit
GTCAGGATGGCCGAGCGGTCTAAGG75700.11040591017275972No Hit
AGCTCTAGAATTACTCTGAGACCT74400.10850990378934378No Hit
TCAGTGCACTACAGAACTTTGTC72490.1057242328721711No Hit
TTTAAACATCCTTGACTGGAAGCT71640.10448453639070683No Hit
TGAGGTAGTAGGTTGTGTGGTT70840.10331776323168163No Hit
ACCACAGGGTAGAACCACGGAC70000.10209265141470517No Hit
TGAGGTAGTAGGTTGTATAGT69780.10177178879597322No Hit
AACATTCAACGCTGTCGGTGAGA69750.10172803480250979No Hit
AGAGGTAGTAGGTTGCATAGTT69080.10075086228182618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)