FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8240851
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG114300713.870011725730752No Hit
CCCCCCACTGCTAAATTTGACTG7121348.641510445947876No Hit
TTCAAGTAATCCAGGATAGGCT3334734.046584509294004No Hit
CCCCCACTGCTAAATTTGACTG3063413.7173466672313333No Hit
TGAGGTAGTAGATTGTATAGTT2252282.7330672523990547No Hit
TAGCAGCACGTAAATATTGGCG2198722.668073964691268No Hit
TAGCTTATCAGACTGATGTTGAC2095652.543001930261814No Hit
TCGTACGACTCTTAGCGG1928312.339940377516837No Hit
AAGCTGCCAGTTGAAGAACTGT1757522.132692363931832No Hit
AACATTCAACGCTGTCGGTGAG1444531.7528893557230922No Hit
TAGCTTATCAGACTGATGTTGA1344871.6319552434572595No Hit
TAGCTTATCAGACTGATGTTGACT1187911.4414894772396685No Hit
GCCTGTCTGAGCGTCGCT1139181.3823572347079203No Hit
TGAGGTAGTAGGTTGTATAGTT956701.1609237929432288No Hit
TATTGCACTTGTCCCGGCCTGT889041.0788206218022873No Hit
CATAAAGTAGAAAGCACTACT871661.0577305669038306No Hit
TTCAAGTAATTCAGGATAGGTT858611.0418948237263361No Hit
TATTGCACTTGTCCCGGCCTGTAA794780.9644392308512798No Hit
TGAGGTAGTAGTTTGTACAGTT743450.9021519743531341No Hit
TATTGCACTTGTCCCGGCCTGTAT739970.8979291095058023No Hit
TGTAAACATCCTTGACTGGAAGCT713540.8658571790704626No Hit
CATTGCACTTGTCTCGGTCTGA555780.6744206393247494No Hit
CACCCGGCTGTGTGCACATGTGC518230.6288549568485099No Hit
CAACGGAATCCCAAAAGCAGCTGT503970.6115509187097303No Hit
AGCAGCATTGTACAGGGCTATGA490480.5951812500917684No Hit
TATTGCACTTGTCCCGGCCTGTA444860.5398228896505955No Hit
AACATTCAACGCTGTCGGTGAGT444610.5395195229230573No Hit
TTCACAGTGGCTAAGTTCCGC402420.48832335398370874No Hit
CAACGGAATCCCAAAAGCAGCT362560.4399545629450162No Hit
TTCTCACTACTGCACTTGACTAG354250.42987065292164606No Hit
ACTGGACTTGGAGTCAGAAGGC303330.3680809178566631No Hit
CCCATAAAGTAGAAAGCACT300960.36520500127960087No Hit
TGATGTAGTAGATTGTATAGTT296580.3598900162131314No Hit
TGTAAACATCCCCGACTGGAAGCT291810.3541017790517023No Hit
AACGGAATCCCAAAAGCAGCTG277570.33682201025112574No Hit
TGAGAACTGAATTCCATGGGTT256840.31166684120365723No Hit
CAACACCAGTCGATGGGCTGT253950.30815992183331553No Hit
CTTCTCACTACTGCACTTGACTAG252140.30596354672593884No Hit
TCCTGTCTGAGCGTCGCT247710.30058788831396177No Hit
TCGTTTCCCGGCCAATGCACCA247580.3004301376156419No Hit
TGAGAACTGAATTCCATAGGCTGT245160.297493547693072No Hit
TGAGGTAGTAGATTGTATAGT238280.2891448953512204No Hit
TAGCAGCACGTAAATATTGGC236120.2865238068252902No Hit
CTCCCACTGCTTCACTTGACTAG232220.28179128587569413No Hit
CCCATAAAGTAGAAAGCACTAC232040.28157286183186664No Hit
TAGCTTATCAGACTGATGTTGACA228150.27685247555137205No Hit
TGTAAACATCCTACACTCTCAGCT205710.24962227808754217No Hit
TGTCAGTTTGTCAAATACCCCA201690.24474414110872775No Hit
CAAAGAATTCTCCTTTTGGGCT197670.2398660041299133No Hit
TTCACAGTGGCTAAGTTCTGC189440.22987917145935538No Hit
TCAGTGCACTACAGAACTTTGT183840.22308375676249942No Hit
TGTAAACATCCCCGACTGGA158650.1925165252957492No Hit
TTATAAAGCAATGAGACTGATT151140.1834033888005013No Hit
TGAGGTAGTAAGTTGTATTGTT148070.17967804538633206No Hit
TGGCTCAGTTCAGCAGGAAC143840.17454508035638552No Hit
ACCCCCCACTGCTAAATTTGACTG138950.16861122716573812No Hit
AACCGGGCGGAAACACCA137570.16693664282972717No Hit
AATCCTTGGAACCTAGGTGTGAGT135640.1645946516931322No Hit
TCAGTGCACTACAGAACTTTGTC135090.16392724489254812No Hit
TTCAAGTAATCCAGGATAGGC131770.15989853475084068No Hit
CCCCCACTGCTAAATTTGACTGGCTT130900.15884281853900767No Hit
CTCCCACTGCTTCACTTGACTAGCCT130760.1586729331715863No Hit
TGAGGTAGTAGTTTGTGCTGTT130290.15810260372381446No Hit
TGATGTAGTAGGTTGTATAGTT128580.15602757530745306No Hit
CCCATAAAGTAGAAAGCACTA127450.15465635769898034No Hit
CAAAGTGCTGTTCGTGCAGGTAG127350.15453501100796507No Hit
TTCAAGTAATCCAGGATAGGCTT126580.1536006414871474No Hit
TTCACAGTGGCTAAGTTCTG124540.15112516899043557No Hit
TGTCAGTTTGTCAAATACCCCAA120360.14605287730599667No Hit
TATTGCACTTGTCCCGGCCTGTT119560.1450821037778744No Hit
CACTAGATTGTGAGCTCCTGGA118750.1440991955806506No Hit
CAAAGAATTCTCCTTTTGGGCTT118260.1435045967946757No Hit
CCCCCCACTGCTAAATTTGACTGG111700.135544253864073No Hit
TAGCAGCACATCATGGTTTACA109380.1327290106325184No Hit
ATCACATTGCCAGGGATTTCC107760.1307631942380708No Hit
TCGTTTCCCGGCCAACGCACCA105230.1276931229553841No Hit
CACTAGATTGTGAGCTCCTGGAA100390.12181994311024431No Hit
TGAGGTAGTAGATTGTATAGTTA99160.12032737881075631No Hit
AGCAGCATTGTACAGGGCTATGT99150.12031524414165479No Hit
ACTGGACTTGGAGTCAGAAGGCT96300.11685686344771917No Hit
TGATGTAGTAGTTTGTACAGTT95490.11587395525049536No Hit
TTCACAGTGGCTAAGTTCCG95010.115291491133622No Hit
GGAATACCGGGTGCTGTAGGCTTT94600.11479396970045933No Hit
TGTCAGTTTGTCAAATACCCC94050.11412656289987526No Hit
TAGCAGCACGTAAATATTGGCGT93950.11400521620885998No Hit
ATGCACCTGGGCAAGGATTCTGA92940.11277961462960562No Hit
AATGCACCCGGGCAAGGATTCT91350.11085020224246259No Hit
CAAAGAATTCTCCTTTTGGGC90550.1098794287143403No Hit
TATTGCACTTGTCCCGGCCTGTAG88010.10679722276255207No Hit
TGTAAACATCCCCGACTGGAAGC82940.10064494552807714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)