FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12503095
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG12119029.692816058743855No Hit
CAACGGAATCCCAAAAGCAGCTG9847277.875865935594346No Hit
TTCAAGTAATCCAGGATAGGCT5420124.3350226483922585No Hit
TAGCAGCACGTAAATATTGGCG4902343.920901184866627No Hit
AAGCTGCCAGTTGAAGAACTGT4604793.6829201089810164No Hit
CCCCCACTGCTAAATTTGACTG4213853.370245527207463No Hit
AACATTCAACGCTGTCGGTGAGT3185322.5476252079984993No Hit
TAGCTTATCAGACTGATGTTGAC2296071.8364013070363778No Hit
TAGCTTATCAGACTGATGTTGACT2192811.7538137557140854No Hit
AACATTCAACGCTGTCGGTGAG2090771.6722019627940121No Hit
TATTGCACTTGTCCCGGCCTGTAT1925891.5403306141399389No Hit
TGAGGTAGTAGGTTGTATAGTT1847561.4776821259056259No Hit
TATTGCACTTGTCCCGGCCTGTAA1788051.4300859107285036No Hit
TAGCTTATCAGACTGATGTTGA1737631.3897598954498867No Hit
CATAAAGTAGAAAGCACTACT1717821.3739158184433533No Hit
TGAGGTAGTAGATTGTATAGTT1490351.191984864547538No Hit
TGTAAACATCCTTGACTGGAAGCT1374041.0989598975293717No Hit
TATTGCACTTGTCCCGGCCTGT1261801.0091901245251675No Hit
TTCAAGTAATTCAGGATAGGTT1144890.9156852763255818No Hit
AGCAGCATTGTACAGGGCTATGA922390.7377293382158577No Hit
TATTGCACTTGTCCCGGCCTGTA856270.684846432023431No Hit
ACTGGACTTGGAGTCAGAAGGC785760.6284523951869517No Hit
CCCATAAAGTAGAAAGCACTAC702540.5618928753240697No Hit
TGTAAACATCCCCGACTGGAAGCT688760.5508716041908024No Hit
CATTGCACTTGTCTCGGTCTGA648730.5188555313704326No Hit
TCGTACGACTCTTAGCGG646330.5169360066447548No Hit
CAACGGAATCCCAAAAGCAGCTGT644300.5153124086476188No Hit
TGTAAACATCCTACACTCTCAGCT606970.48545580114363684No Hit
CACCCGGCTGTGTGCACATGTGC605980.4846639971942947No Hit
TGAGGTAGTAGTTTGTACAGTT576600.46116581534412077No Hit
CCCATAAAGTAGAAAGCACT563860.4509763382586472No Hit
TTCTCACTACTGCACTTGACTAG504320.40335612902245405No Hit
CTTCTCACTACTGCACTTGACTAG426960.3414834486981023No Hit
CAAAGAATTCTCCTTTTGGGCT401520.32113648660591637No Hit
TGTCAGTTTGTCAAATACCCCAA391990.313514373841037No Hit
CAACGGAATCCCAAAAGCAGCT383710.3068920135374481No Hit
CTCCCACTGCTTCACTTGACTAG366130.29283149492185734No Hit
TCAGTGCACTACAGAACTTTGT347890.2782431070067051No Hit
CAACACCAGTCGATGGGCTGT325950.2606954518061328No Hit
TGAGGTAGTAGTTTGTGCTGTT319170.2552727944560927No Hit
CACTAGATTGTGAGCTCCTGGA318850.2550168578260023No Hit
TGTCAGTTTGTCAAATACCCCA308910.24706682625381957No Hit
AGCTCGGTCTGAGGCCCCTCAGT308600.24681888764341947No Hit
CAAAGAATTCTCCTTTTGGGCTT299860.23982861843407574No Hit
ATCCCGGACGAGCCCCCA295700.23650144224290065No Hit
TGAGAACTGAATTCCATGGGTT295300.23618152145528765No Hit
TTCAAGTAATCCAGGATAGGCTT292220.23371813139066766No Hit
TTAATGCTAATCGTGATAGGGGTT285430.22828747602093724No Hit
CACTAGATTGTGAGCTCCTGGAA269180.21529069402415965No Hit
TTCACAGTGGCTAAGTTCCG257630.20605298128183464No Hit
CGCCTGTCTGAGCGTCGCT256020.20476530011169233No Hit
TTCACAGTGGCTAAGTTCCGC255780.20457334763912455No Hit
TTCAAGTAATCCAGGATAGGC254730.20373355557164047No Hit
AACGGAATCCCAAAAGCAGCTG253130.2024538724211885No Hit
TAGCAGCACGTAAATATTGGC252740.20214194965326582No Hit
TATTGCACTTGTCCCGGCCTGTT251900.20147011599927858No Hit
TCGTTTCCCGGCCAATGCACCA242670.1940879438251089No Hit
AACATTCAACGCTGTCGGTGAGTT242370.19384800323439916No Hit
TATTGCACTTGTCCCGGCCTGTAG241500.19315217552134092No Hit
TGTAAACATCCCCGACTGGAAGC234470.1875295676790427No Hit
TTATAAAGCAATGAGACTGATT227960.1823228568606413No Hit
AACCGGGCGGAAACACCA226150.18087521529669254No Hit
TCGTTTCCCGGGCGGCGCACCA225850.1806352747059828No Hit
TGTAAACATCCTACACTCAGCT223420.1786917559212339No Hit
TAGCTTATCAGACTGATGTTGACA222480.1779399420703434No Hit
TGAGAACTGAATTCCATAGGCTGT213240.17054977187648337No Hit
TGTCAGTTTGTCAAATACCCC210800.16859825507204415No Hit
TTCACAGTGGCTAAGTTCTGC207420.1658949244167144No Hit
ACTGGACTTGGAGTCAGAAGGCT202120.16165597398084233No Hit
CCCATAAAGTAGAAAGCACTA200090.16003237598370645No Hit
ACTGGACTTGGAGTCAGAAGGCA193090.15443376220047916No Hit
CCCCCCACTGCTAAATTTGACTGGCT190570.15241826123851734No Hit
AGCAGCATTGTACAGGGCTATGT189850.151842403820814No Hit
TATTGCACTTGTCCCGGCCTGTTT186640.14927503950021975No Hit
CCCCCACTGCTAAATTTGACTGGCTT184910.14789138209379357No Hit
ACTGGACTTGGAGTCAGAAGG178470.14274065741322448No Hit
CTACGCCTGTCTGAGCGTCGCT177750.14216479999552112No Hit
GCCTGTCTGAGCGTCGCT174100.1392455228085526No Hit
TCAGTGCACTACAGAACTTTGTC173050.13840573074106852No Hit
CCCCCCACTGCTAAATTTGACTGG171800.13740597827977793No Hit
CAGTGCAATAGTATTGTCAAAGC163470.13074362787773747No Hit
AGAGGTAGTAGGTTGCATAGTT161120.12886409325051118No Hit
TCAGTGCATCACAGAACTTTGTTT160650.12848818632506592No Hit
TGAGGTAGTAGGTTGTATAGT155360.12425723390888416No Hit
ACCCCCCACTGCTAAATTTGACTG155180.12411326955445831No Hit
ATCACATTGCCAGGGATTTCC155140.12408127747569701No Hit
CCCCCCACTGCTAAATTTGACTGT151350.12105002801306396No Hit
TGAGGTAGTAAGTTGTATTGTT150240.12016224782743794No Hit
CAAAGTGCTGTTCGTGCAGGTAG148750.11897054289357954No Hit
ACCACAGGGTAGAACCACGGAC148420.11870660824379883No Hit
TATTGCACTTGTCCCGGCCTGTAAT144540.11560337660395285No Hit
TTCACAGTGGCTAAGTTCTG144240.11536343601324311No Hit
AGCAGCATTGTACAGGGCTATG142680.11411574494155247No Hit
CAAAGTGCTGTTCGTGCAGGT140760.11258012516101013No Hit
TACGCCTGTCTGAGCGTCGCT139300.11141241428622273No Hit
TGTCAGTTTGTCAAATACCC138780.11099651726232584No Hit
TAGCAGCACATCATGGTTTACA136100.10885304798531883No Hit
TAGCAGCACGTAAATATTGGCGT135540.10840515888266065No Hit
CCCCCCACTGCTAAATTTGACTGA134660.10770133314991208No Hit
CTCCCACTGCTTCACTTGACTAGCCT131930.10551787377445344No Hit
TGATGTAGTAGGTTGTATAGTT131910.10550187773507279No Hit
TGTCAGTTTGTCAAATACC130160.10410222428926597No Hit
TATTGCACTTGTCCCGGCCTGA128820.10303048965076247No Hit
TGAGGTAGTAGATTGTATAGT128420.10271056886314948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)