FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898693
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG89851610.097168202116872No Hit
TTCAAGTAATCCAGGATAGGCT4206614.727222301072754No Hit
TGAGGTAGTAGATTGTATAGTT3952694.441877026210479No Hit
CCCCCCACTGCTAAATTTGACTG3870344.349335346213203No Hit
TAGCAGCACGTAAATATTGGCG2605742.9282277745731875No Hit
CATAAAGTAGAAAGCACTACT2316872.603607069038116No Hit
CCCCCACTGCTAAATTTGACTG2166022.4340877924432274No Hit
AAGCTGCCAGTTGAAGAACTGT2052442.306451070960646No Hit
TAGCTTATCAGACTGATGTTGAC1856542.086306382296816No Hit
TAGCTTATCAGACTGATGTTGA1805722.0291968719451274No Hit
TGAGGTAGTAGGTTGTATAGTT1791732.013475462070666No Hit
GCCTGTCTGAGCGTCGCT1538411.7288044435289542No Hit
TGAGAACTGAATTCCATGGGTT1386631.558240069637193No Hit
AACATTCAACGCTGTCGGTGAG1259911.4158371347342806No Hit
TGAGGTAGTAGTTTGTACAGTT1246771.4010709213139503No Hit
TTCAAGTAATTCAGGATAGGTT1229851.3820568930740729No Hit
TATTGCACTTGTCCCGGCCTGT1094321.229753627864227No Hit
TAGCTTATCAGACTGATGTTGACT1045591.174992777029166No Hit
TTCACAGTGGCTAAGTTCCGC986541.108634717480421No Hit
TGATGTAGTAGATTGTATAGTT982561.1041621505540196No Hit
TGTAAACATCCTTGACTGGAAGCT692330.7780131306923388No Hit
TATTGCACTTGTCCCGGCCTGTAA627320.7049574583593343No Hit
TATTGCACTTGTCCCGGCCTGTAT597600.6715592952807788No Hit
CATTGCACTTGTCTCGGTCTGA562000.6315534202607057No Hit
TGAGAACTGAATTCCATAGGCTGT538290.6049090579931232No Hit
TGAGGTAGTAGATTGTATAGT468660.5266616119917835No Hit
AACATTCAACGCTGTCGGTGAGT467690.52557156427354No Hit
TTAATGCTAATCGTGATAGGGGTT459220.5160533125482585No Hit
TGATGTAGTAGGTTGTATAGTT445760.5009274957569612No Hit
CAACGGAATCCCAAAAGCAGCTGT440870.49543230674437244No Hit
TCCTGTCTGAGCGTCGCT422790.4751147162847398No Hit
TATTGCACTTGTCCCGGCCTGTA408550.4591123662767105No Hit
CACCCGGCTGTGTGCACATGTGC391300.4397274970605234No Hit
AGCAGCATTGTACAGGGCTATGA383170.4305913239168943No Hit
CAACACCAGTCGATGGGCTGT346160.38900094654349804No Hit
TGAGGTAGTAAGTTGTATTGTT344290.3868995143444099No Hit
TGATAACTGAATTCCATGGGTT321720.3615362390858972No Hit
TGATGTAGTAGTTTGTACAGTT303330.3408702828606403No Hit
CCCATAAAGTAGAAAGCACTAC302370.33979147274773946No Hit
TTCTCACTACTGCACTTGACTAG287600.3231935296565462No Hit
AACCGGGCGGAAACACCA282040.31694542108599544No Hit
TGTAAACATCCCCGACTGGA280170.31484398888690734No Hit
TCGTTTCCCGGCCAATGCACCA249940.28087270793587327No Hit
TTATAAAGCAATGAGACTGATT246390.2768833580392087No Hit
TGGCTCAGTTCAGCAGGAAC243350.27346712601502265No Hit
TTCACAGTGGCTAAGTTCTGC239110.2687023813497106No Hit
TAGCAGCACGTAAATATTGGC238360.2678595609490068No Hit
TGTAAACATCCCCGACTGGAAGCT232580.2613642250609163No Hit
TGAGGTAGTAGTTTGTGCTGTT225110.2529697338699065No Hit
TGTAAACATCCTACACTCTCAGCT215660.24235019682103878No Hit
AACGGAATCCCAAAAGCAGCTG210390.23642797880542682No Hit
ACTGGACTTGGAGTCAGAAGGC201040.22592081780998624No Hit
CAACGGAATCCCAAAAGCAGCT199140.22378567279487No Hit
TTCACAGTGGCTAAGTTCCG190290.21384039206656527No Hit
TTCACAGTGGCTAAGTTCTG185670.20864861839822993No Hit
TGTCAGTTTGTCAAATACCCCA182720.20533352482212835No Hit
TAGCTTATCAGACTGATGTTGACA174490.19608497562507216No Hit
TTCAAGTAATCCAGGATAGGC173160.19459037411449076No Hit
TCAGTGCACTACAGAACTTTGT171300.19250017952074536No Hit
TGTCAGTTTGTCAAATACC168330.18916261073395835No Hit
CAAAGAATTCTCCTTTTGGGCT165790.18630825897690817No Hit
CCCATAAAGTAGAAAGCACT150610.1692495740666635No Hit
TGAGGTAGTAGATTGTATAGTTA135460.15222460197244694No Hit
TATTGCACTTGTCCCGGCCTGTT133500.1500220313252744No Hit
CTTCTCACTACTGCACTTGACTAG129850.1459203053751826No Hit
TCGACCGGACCTCGACCGGCT126590.1422568460334568No Hit
ATCACATTGCCAGGGATTTCC126490.14214446998002964No Hit
TGATAACTGAATTCCATAGGCTGT124020.1393687814603785No Hit
TTCAAGTAATCCAGGATAGGCTT123290.13854843627036015No Hit
TGAGGTAGTAGGTTGTATAGT121710.13677289462621084No Hit
TAGCTTATCAGACTGATGTTG120790.13573903493468087No Hit
ACCGGGTGCTGTAGGCTT119030.13376121639436264No Hit
TGTCAGTTTGTCAAATACCC118590.13326676175928306No Hit
TAGCAGCACATCATGGTTTACA117070.13155864574719006No Hit
TGATGTAGTAGATTGTATAGT116890.13135636885102114No Hit
CCCCCACTGCTAAATTTGACTGGCTT116260.13064839971442996No Hit
CTCCCACTGCTTCACTTGACTAG115540.12983929212975434No Hit
AGCTACATTGTCTGCTGGGTTT115050.1292886494679612No Hit
TAGCAGCACGTAAATATTGGCGT113680.12774909753600894No Hit
CAAAGTGCTGTTCGTGCAGGTAG112640.12658038658036636No Hit
TGAGAACTGAATTCCATGGGTTG111720.12554652688883639No Hit
TGTCAGTTTGTCAAATACCCC110700.12440029114387922No Hit
TGTCAGTTTGTCAAATACCCCAA108570.12200668120588047No Hit
TTCACAGTGGCTAAGTTCCGCT107070.12032104040447288No Hit
AGGTCCCTGTTCGGGCGCCA106550.11973668492665158No Hit
TGTAAACATCCTACACTCAGCT106430.11960183366253897No Hit
CAAAGAATTCTCCTTTTGGGCTT105110.11811846975730031No Hit
TCGTTTCCCGGCCAACGCACCA104450.11737678780468097No Hit
TCAGTGCACTACAGAACTTTGTC103930.1167924323268597No Hit
TAGCAGCACATAATGGTTTGT102440.11511802913079482No Hit
ACCGGGTGCTGTAGGCTTT97200.10922952393121102No Hit
CACTAGATTGTGAGCTCCTGGA94970.10672353793978509No Hit
CTCCTGGCTGGCTCGCCA94180.10583576711771044No Hit
TCCACCACGTTCCCGTGG90910.10216107017064191No Hit
TGAGGTAGTAGTTTGTGCTGTTA89890.10101483442568476No Hit
TGAGGTAGTAGTTTGTACAGT89190.10022820205169455No Hit
TGTAAACATCCTTGACTGGA89180.10021696444635184No Hit
CGCCTGTCTGAGCGTCGCT88990.10000344994484023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)