FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6155072
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG85427513.879204012560697No Hit
CAACGGAATCCCAAAAGCAGCTG5835009.479986586671934No Hit
TTCAAGTAATCCAGGATAGGCT2737154.4469829110041275No Hit
CCCCCACTGCTAAATTTGACTG2521274.096247777442733No Hit
TAGCAGCACGTAAATATTGGCG2509404.076962868996496No Hit
AAGCTGCCAGTTGAAGAACTGT2292893.7252041893254866No Hit
AACATTCAACGCTGTCGGTGAGT1916423.1135622783941437No Hit
AACATTCAACGCTGTCGGTGAG1074851.7462833903486426No Hit
TAGCTTATCAGACTGATGTTGAC1031261.6754637476214738No Hit
TAGCTTATCAGACTGATGTTGACT913591.4842880798145008No Hit
TGAGGTAGTAGGTTGTATAGTT722041.1730813222006178No Hit
TATTGCACTTGTCCCGGCCTGTAA690881.1224564066837885No Hit
TATTGCACTTGTCCCGGCCTGTAT634421.030727179145914No Hit
TGTAAACATCCTTGACTGGAAGCT564320.9168373659966934No Hit
TAGCTTATCAGACTGATGTTGA557440.9056595926091522No Hit
CATAAAGTAGAAAGCACTACT531350.863271786260177No Hit
TATTGCACTTGTCCCGGCCTGT448210.728196193318291No Hit
TGAGGTAGTAGATTGTATAGTT380930.6188879675168706No Hit
AGCAGCATTGTACAGGGCTATGA369030.599554318779699No Hit
CCCATAAAGTAGAAAGCACTAC367830.5976047071423373No Hit
CAACGGAATCCCAAAAGCAGCTGT359810.5845748026993023No Hit
TTAATGCTAATCGTGATAGGGGTT336800.5471909995528891No Hit
TATTGCACTTGTCCCGGCCTGTA335880.5456962972975783No Hit
CTACGCCTGTCTGAGCGTCGCT300810.4887188971956786No Hit
TGTAAACATCCTACACTCTCAGCT297130.48274008817443564No Hit
TTCAAGTAATTCAGGATAGGTT284710.4625616077277407No Hit
CGCCTGTCTGAGCGTCGCT266180.43245635469414495No Hit
ACTGGACTTGGAGTCAGAAGGC242050.39325291401952733No Hit
TGTAAACATCCCCGACTGGAAGCT235300.38228634855936694No Hit
TGAGGTAGTAGTTTGTACAGTT195370.31741302132615185No Hit
TACGCCTGTCTGAGCGTCGCT189710.3082173531032618No Hit
CATTGCACTTGTCTCGGTCTGA185230.3009388029904443No Hit
TTCACAGTGGCTAAGTTCCG181910.2955448774604099No Hit
CAAAGAATTCTCCTTTTGGGCT178930.29070334189429464No Hit
TGTCAGTTTGTCAAATACCCCAA176360.2865279236376114No Hit
GGAATACCGGGTGCTGTAGGCTTT167810.2726369407214083No Hit
CAACGGAATCCCAAAAGCAGCT165620.26907889948322294No Hit
AACATTCAACGCTGTCGGTGAGTT161690.26269392137086295No Hit
GCCTGTCTGAGCGTCGCT155830.2531733178750793No Hit
CCCCCACTGCTAAATTTGACTGGCTT155060.2519223170744388No Hit
CCCATAAAGTAGAAAGCACT153680.24968026369147264No Hit
TAGCAGCACATCATGGTTTACA143190.2326374086282013No Hit
TAGCTTATCAGACTGATGTTGACA142830.2320525251369927No Hit
TTCACAGTGGCTAAGTTCCGC125400.20373441610431203No Hit
AACGGAATCCCAAAAGCAGCTG123310.20033884250257347No Hit
GGAATACCGGGTGCTGTAGGCTT121690.197706866792135No Hit
CACCCGGCTGTGTGCACATGTGC121480.19736568475559665No Hit
TGTAAACATCCTACACTCAGCT118900.19317401973526874No Hit
TGTCAGTTTGTCAAATACCCCA118540.19258913624406018No Hit
TGAGGTAGTAGTTTGTGCTGTT118400.19236168155303462No Hit
TAGCAGCACGTAAATATTGGCGT118120.19190677217098354No Hit
CAACACCAGTCGATGGGCTGT117750.1913056419161303No Hit
CCCCCCACTGCTAAATTTGACTGG117140.1903145893338047No Hit
TCGTACGACTCTTAGCGG114610.1862041581317002No Hit
TAGCAGCACGTAAATATTGGC114240.18560302787684693No Hit
AACCGGGCGGAAACACCA113010.18360467594855107No Hit
ACGCCTGTCTGAGCGTCGCT112380.1825811298389361No Hit
TCGTTTCCCGGCCAATGCACCA109080.17721969783619104No Hit
TTCAAGTAATCCAGGATAGGC104460.16971369303234796No Hit
TATTGCACTTGTCCCGGCCTGTT103970.16891760161375854No Hit
TTCTCACTACTGCACTTGACTAG102510.16654557412163498No Hit
TCCACCACGTTCCCGTGG102320.16623688561238603No Hit
ACCCCCCACTGCTAAATTTGACTG94190.15302826676925957No Hit
ATCACATTGCCAGGGATTTCC91940.14937274494920613No Hit
ACTGGACTTGGAGTCAGAAGGCA90700.14735814625726557No Hit
CCCCCCACTGCTAAATTTGACTGGCT88450.14370262443721213No Hit
TTCAAGTAATCCAGGATAGGCTT87170.14162303869069282No Hit
TCAGGCTCAGTCCCCTCCCGAT86100.13988463498071185No Hit
TATTGCACTTGTCCCGGCCTGTAG85980.13968967381697567No Hit
CTTCTCACTACTGCACTTGACTAG83710.13600165846963286No Hit
TTCACAGTGGCTAAGTTCTGC83630.1358716843604754No Hit
CAAAGAATTCTCCTTTTGGGCTT81050.13168001934014745No Hit
ATCCCGGACGAGCCCCCA78860.12812197810196208No Hit
TGAGGTAGTAGGTTGTGTGGTT77380.12571745708254914No Hit
AACATTCAACGCTGTCGGTGAGA71660.11642430827779107No Hit
TCAGGCTCAGTCCCCTCCCGA70830.11507582689528247No Hit
GGGCTACGCCTGTCTGAGCGTCGCT66990.1088370696557246No Hit
ATCACATTGCCAGGGATTTCCA66490.1080247314734905No Hit
CTCGTACGACTCTTAGCGG66370.10782977030975431No Hit
AGAGGTAGTAGGTTGCATAGTT65570.10653002921817974No Hit
ACTGGACTTGGAGTCAGAAGGCT64470.10474288521726471No Hit
CCCATAAAGTAGAAAGCACTA63540.10323193619830928No Hit
CAAAGTGCTGTTCGTGCAGGTAG61620.10011255757853037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)