FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11609885
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8195367.058950196319774No Hit
CAACGGAATCCCAAAAGCAGCTG6382185.497194847321916No Hit
TAGCAGCACGTAAATATTGGCG4473013.852759954125299No Hit
AAGCTGCCAGTTGAAGAACTGT3922253.3783711035897426No Hit
TTCAAGTAATCCAGGATAGGCT3777133.253374172095589No Hit
TAGCTTATCAGACTGATGTTGAC3474012.9922863146361918No Hit
CCCCCACTGCTAAATTTGACTG3327342.8659543139316193No Hit
AACATTCAACGCTGTCGGTGAGT3105722.6750652568910027No Hit
TAGCTTATCAGACTGATGTTGACT2214651.9075555011957481No Hit
AACATTCAACGCTGTCGGTGAG1947131.6771311688272537No Hit
TGTAAACATCCTTGACTGGAAGCT1739231.4980596276362772No Hit
TGAGGTAGTAGGTTGTATAGTT1683701.450229696504315No Hit
TAGCTTATCAGACTGATGTTGA1522881.3117098059110834No Hit
TATTGCACTTGTCCCGGCCTGTAA1393001.199839619427755No Hit
AGCAGCATTGTACAGGGCTATGA1219001.0499673338710935No Hit
CATAAAGTAGAAAGCACTACT1210981.0430594273758955No Hit
TATTGCACTTGTCCCGGCCTGTAT1198451.032266900145867No Hit
CGCCTGTCTGAGCGTCGCT1125850.9697339809998118No Hit
TGAGGTAGTAGATTGTATAGTT1117790.9627916211056355No Hit
TATTGCACTTGTCCCGGCCTGT882030.7597232875261039No Hit
TTCAAGTAATTCAGGATAGGTT860650.7413079457720727No Hit
ACTGGACTTGGAGTCAGAAGGC811800.6992317322695272No Hit
TGTCAGTTTGTCAAATACCCCAA725400.6248123904758747No Hit
TATTGCACTTGTCCCGGCCTGTA713820.6148381314715865No Hit
TGTAAACATCCCCGACTGGAAGCT634630.5466290148438163No Hit
CATTGCACTTGTCTCGGTCTGA527390.45425945218234287No Hit
GCCTGTCTGAGCGTCGCT516070.4445091402714153No Hit
TGTAAACATCCTACACTCTCAGCT499100.4298922857547685No Hit
TGAGGTAGTAGTTTGTACAGTT468120.40320812824588703No Hit
TGTCAGTTTGTCAAATACCCCA417900.3599518858283265No Hit
TACGCCTGTCTGAGCGTCGCT414740.3572300673090216No Hit
TTATAAAGCAATGAGACTGATT370390.3190298611915622No Hit
CAACGGAATCCCAAAAGCAGCTGT366550.3157223348896221No Hit
CAACACCAGTCGATGGGCTGT363280.3129057695231262No Hit
TAGCTTATCAGACTGATGTTGACA345280.2974017399827819No Hit
GCGTTCGCGCTTTCCCCTG338470.291536048806685No Hit
TTCTCACTACTGCACTTGACTAG327550.28213027088554277No Hit
TTCACAGTGGCTAAGTTCCG315840.27204403833457436No Hit
TGTAAACATCCTACACTCAGCT311560.2683575246438703No Hit
AACATTCAACGCTGTCGGTGAGTT308940.2661008270107757No Hit
CTACGCCTGTCTGAGCGTCGCT306850.26430063691414685No Hit
CCCATAAAGTAGAAAGCACTAC297640.25636774179933736No Hit
CACCCGGCTGTGTGCACATGTGC294420.2535942431815647No Hit
TGAGGTAGTAGTTTGTGCTGTT288600.24858127363018667No Hit
TAGCAGCACGTAAATATTGGC284170.24476555969331304No Hit
CTTCTCACTACTGCACTTGACTAG262890.22643635143672827No Hit
CCCATAAAGTAGAAAGCACT246170.21203483066369733No Hit
AGAATTACTCTGAGACCT242460.20883927790843754No Hit
TTCAAGTAATCCAGGATAGGCTT236050.20331812072212604No Hit
CAAAGAATTCTCCTTTTGGGCT235440.20279270638770322No Hit
AGCAGCATTGTACAGGGCTATGT228950.19720264240343466No Hit
TAGCAGCACGTAAATATTGGCGT225090.1938778894020053No Hit
ACTGGACTTGGAGTCAGAAGG221320.1906306565482776No Hit
CAAAGAATTCTCCTTTTGGGCTT218990.18862374605777749No Hit
ACTGGACTTGGAGTCAGAAGGCA218940.1885806793090543No Hit
CAACGGAATCCCAAAAGCAGCT216270.18628091492723658No Hit
ACGCCTGTCTGAGCGTCGCT215550.1856607537456228No Hit
TCAGTGCACTACAGAACTTTGT212480.18301645537401964No Hit
TTCACAGTGGCTAAGTTCCGC202480.1744031056293839No Hit
AGCAGCATTGTACAGGGCTATG199510.1718449407552271No Hit
TCGTTTCCCGGCCAATGCACCA199160.17154347351416488No Hit
AACCGGGCGGAAACACCA196170.1689680819405188No Hit
TGTAAACATCCCCGACTGGAAGC186710.1608198530820934No Hit
CCCCCACTGCTAAATTTGACTGGCTT182470.15716779279036786No Hit
AACGGAATCCCAAAAGCAGCTG177370.15277498442060367No Hit
CAAAGTGCTGTTCGTGCAGGTAG176780.15226679678567015No Hit
ACTGGACTTGGAGTCAGAAGGCT173900.14978615205921506No Hit
ACCACAGGGTAGAACCACGGAC173830.1497258586110026No Hit
TTCACAGTGGCTAAGTTCTG172090.148227135755436No Hit
TTCAAGTAATCCAGGATAGGC171720.14790844181488447No Hit
TTCACAGTGGCTAAGTTCTGC169370.1458843046248951No Hit
TGTCAGTTTGTCAAATACCCC168590.14521246334481347No Hit
TATTGCACTTGTCCCGGCCTGTT167740.14448032861651947No Hit
TATTGCACTTGTCCCGGCCTGTAG162970.14037176078832822No Hit
TGTAAACATCCTTGACTGGAAGC160170.13796002285983022No Hit
CAGTGCAATAGTATTGTCAAAGC158190.13625457961039236No Hit
TCAGTGCACTACAGAACTTTGTC152010.1309315294682075No Hit
CCTCGACACAAGGGTTTGT150870.129949607597319No Hit
CACTAGATTGTGAGCTCCTGGA150360.12951032676034258No Hit
ATCACATTGCCAGGGATTTCCA148250.12769290996422444No Hit
ATCACATTGCCAGGGATTTCC142010.12231817972357176No Hit
CCAAGTCCTTCTGATCGAGGCCC141660.12201671248250952No Hit
AGAGGTAGTAGGTTGCATAGTT140940.12139655130089574No Hit
TGAGGTAGTAGGTTGTATAGT140770.12125012435523694No Hit
CACTAGATTGTGAGCTCCTGGAA136530.1175980640635114No Hit
CAAAGTGCTGTTCGTGCAGGT132680.11428192441182664No Hit
CTCCCACTGCTTCACTTGACTAG131970.11367037657995752No Hit
TGAGGTAGTAGGTTGTGTGGTT127090.10946706190457528No Hit
TAGCAGCACATCATGGTTTACA126920.10932063495891647No Hit
GGAATACCGGGTGCTGTAGGCTTT126450.10891580752091859No Hit
TAGCAGCACATAATGGTTTGT126160.10866602037832415No Hit
CCCCCCACTGCTAAATTTGACTGGCT125670.108243966240837No Hit
TGAGGTAGTAAGTTGTATTGTT124260.10702948392684336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)