FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076601
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG135546212.237165534806211No Hit
CCCCCCACTGCTAAATTTGACTG7428756.706705423441722No Hit
TTCAAGTAATCCAGGATAGGCT5844015.2759957680158385No Hit
CCCCCACTGCTAAATTTGACTG3981433.5944510414341004No Hit
TAGCAGCACGTAAATATTGGCG3618143.2664713660806233No Hit
TGAGGTAGTAGATTGTATAGTT3535253.1916379401948305No Hit
TAGCTTATCAGACTGATGTTGAC2995522.704367522130661No Hit
TAGCTTATCAGACTGATGTTGA2647602.3902639446884475No Hit
AAGCTGCCAGTTGAAGAACTGT2610362.3566435226835383No Hit
CATAAAGTAGAAAGCACTACT2273752.0527506587986695No Hit
TGAGGTAGTAGGTTGTATAGTT1998071.8038656443434227No Hit
AACATTCAACGCTGTCGGTGAG1755201.5846016300487848No Hit
TAGCTTATCAGACTGATGTTGACT1396391.2606665167410112No Hit
TTCAAGTAATTCAGGATAGGTT1338821.2086920888456667No Hit
TTCACAGTGGCTAAGTTCCGC1237901.1175811063339738No Hit
TGAGGTAGTAGTTTGTACAGTT1221211.1025133071056725No Hit
TATTGCACTTGTCCCGGCCTGT1216541.0982972122946382No Hit
TGTAAACATCCTTGACTGGAAGCT966680.8727225978438693No Hit
TGATGTAGTAGATTGTATAGTT893060.8062581652981812No Hit
GCCTGTCTGAGCGTCGCT765910.6914666331305064No Hit
TATTGCACTTGTCCCGGCCTGTAA677680.6118122337348795No Hit
TATTGCACTTGTCCCGGCCTGTAT677610.6117490374529154No Hit
CATTGCACTTGTCTCGGTCTGA651220.587924039152444No Hit
AGCAGCATTGTACAGGGCTATGA602410.5438581745428945No Hit
CAACGGAATCCCAAAAGCAGCTGT597660.5395698554096152No Hit
AACATTCAACGCTGTCGGTGAGT594690.5368885274462807No Hit
TTCTCACTACTGCACTTGACTAG533450.4816008087679605No Hit
TATTGCACTTGTCCCGGCCTGTA514060.4640954386639006No Hit
TGATGTAGTAGGTTGTATAGTT504200.45519379094724094No Hit
TGAGGTAGTAGATTGTATAGT417120.3765776161838817No Hit
TGAGAACTGAATTCCATGGGTT404680.3653467340748303No Hit
TAGCAGCACGTAAATATTGGC343800.3103840248466113No Hit
CAACGGAATCCCAAAAGCAGCT337450.3046512192684381No Hit
CAACACCAGTCGATGGGCTGT329030.29704960935218305No Hit
TGTAAACATCCCCGACTGGA328740.296787796184046No Hit
TGTCAGTTTGTCAAATACCCCA328490.2965620951770313No Hit
CACCCGGCTGTGTGCACATGTGC321680.29041399974595095No Hit
CTTCTCACTACTGCACTTGACTAG321480.2902334389403392No Hit
CCCATAAAGTAGAAAGCACTAC317440.2865861106669817No Hit
TCGTACGACTCTTAGCGG315030.28441035295936No Hit
TGTAAACATCCCCGACTGGAAGCT307700.2777927994336891No Hit
AACGGAATCCCAAAAGCAGCTG301720.27239403134589757No Hit
TGTAAACATCCTACACTCTCAGCT300330.2711391337468958No Hit
TGATGTAGTAGTTTGTACAGTT299640.27051619896753526No Hit
TAGCTTATCAGACTGATGTTGACA298990.26992937634929703No Hit
TGAGGTAGTAAGTTGTATTGTT289800.2616326073314368No Hit
CTCCCACTGCTTCACTTGACTAG281400.25404905349574297No Hit
AACCGGGCGGAAACACCA263550.23793400159489358No Hit
TTATAAAGCAATGAGACTGATT260330.2350269726245443No Hit
CCCCCACTGCTAAATTTGACTGGCTT260010.23473807533556548No Hit
TTCACAGTGGCTAAGTTCTGC257290.2322824483792456No Hit
CAAAGAATTCTCCTTTTGGGCT255530.23069351328986212No Hit
TGAGAACTGAATTCCATAGGCTGT249790.22551141816880468No Hit
ACTGGACTTGGAGTCAGAAGGC244490.22072655682009312No Hit
TTCAAGTAATCCAGGATAGGC236660.2136576012803928No Hit
TGGCTCAGTTCAGCAGGAAC229130.2068594869491101No Hit
ATCACATTGCCAGGGATTTCC221460.1999349800538992No Hit
TCCTGTCTGAGCGTCGCT217190.19608000685408816No Hit
CCCATAAAGTAGAAAGCACT216050.19505081026210117No Hit
TTCACAGTGGCTAAGTTCCG205030.18510190987289332No Hit
TCGTTTCCCGGCCAATGCACCA202120.18247475015124223No Hit
ACCCCCCACTGCTAAATTTGACTG196230.177157234425976No Hit
TGAGGTAGTAGTTTGTGCTGTT192690.17396130816664787No Hit
TTCAAGTAATCCAGGATAGGCTT181020.1634255851592018No Hit
TTCACAGTGGCTAAGTTCTG171270.15462324588562865No Hit
TGTCAGTTTGTCAAATACCCC164910.14888141226717472No Hit
TAGCTTATCAGACTGATGTTG162770.14694941164712894No Hit
TGTCAGTTTGTCAAATACC162000.14625425254552366No Hit
CAAAGTGCTGTTCGTGCAGGTAG160430.14483685022147136No Hit
TATTGCACTTGTCCCGGCCTGTT155430.1403228300811774No Hit
TGAGGTAGTAGATTGTATAGTTA154670.13963669901985276No Hit
TAGCAGCACATCATGGTTTACA154080.13910404464329806No Hit
TGTAAACATCCTACACTCAGCT153580.13865264262926869No Hit
CCCCCCACTGCTAAATTTGACTGG152340.13753316563447576No Hit
TAGCAGCACGTAAATATTGGCGT148010.13362402419298122No Hit
CTCCCACTGCTTCACTTGACTAGCCT147340.1330191454941818No Hit
TGTCAGTTTGTCAAATACCC147190.13288372488997302No Hit
CAAAGAATTCTCCTTTTGGGCTT146190.13198092086191424No Hit
TGAGGTAGTAGGTTGTATAGT144230.130211424966919No Hit
TGTCAGTTTGTCAAATACCCCAA139000.12548975990017155No Hit
TCAGTGCACTACAGAACTTTGT137620.12424389034145042No Hit
AATCCTTGGAACCTAGGTGTGAGT135000.12187854378793639No Hit
TAGCAGCACATAATGGTTTGT129070.11652491590154777No Hit
CACTAGATTGTGAGCTCCTGGA127040.11469222372458843No Hit
AGCAGCATTGTACAGGGCTATGT124340.11225465284882971No Hit
CCCCCCACTGCTAAATTTGACTGGCT123390.11139698902217386No Hit
CAAAGAATTCTCCTTTTGGGC122310.11042196067187038No Hit
TCGTTTCCCGGCCAACGCACCA116740.10539334223558293No Hit
TGTAAACATCCTTGACTGGA113750.10269395819168714No Hit
AGCTCGGTCTGAGGCCCCTCAGT113580.10254048150691714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)