FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13447466
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG10103617.5133932296240795No Hit
CAACGGAATCCCAAAAGCAGCTG7952515.913761001515081No Hit
TTCAAGTAATCCAGGATAGGCT5678954.2230632893959354No Hit
AAGCTGCCAGTTGAAGAACTGT5096843.7901861956743375No Hit
TAGCAGCACGTAAATATTGGCG4671173.47364328714421No Hit
CCCCCACTGCTAAATTTGACTG4267583.1735198289402624No Hit
TAGCTTATCAGACTGATGTTGAC3908462.9064657980916255No Hit
AACATTCAACGCTGTCGGTGAGT3368092.504628009470334No Hit
TAGCTTATCAGACTGATGTTGACT3273342.4341686381657333No Hit
TAGCTTATCAGACTGATGTTGA3167802.355685450329452No Hit
TGAGGTAGTAGGTTGTATAGTT2386461.7746540500641532No Hit
CATAAAGTAGAAAGCACTACT2280221.6956503180599232No Hit
AACATTCAACGCTGTCGGTGAG2254911.6768289282159181No Hit
TGTAAACATCCTTGACTGGAAGCT2227441.6564012877965262No Hit
TGAGGTAGTAGATTGTATAGTT2071011.5400745389503123No Hit
TTCAAGTAATTCAGGATAGGTT1563041.1623305089598293No Hit
TATTGCACTTGTCCCGGCCTGTAA1381441.0272864791032006No Hit
TATTGCACTTGTCCCGGCCTGTAT1346491.0012964524320047No Hit
TGAGGTAGTAGTTTGTACAGTT957610.7121118581002548No Hit
TATTGCACTTGTCCCGGCCTGT921500.6852592153793138No Hit
AGCAGCATTGTACAGGGCTATGA916240.6813476977744357No Hit
TGTCAGTTTGTCAAATACCCCAA798720.593955768320961No Hit
TGTAAACATCCCCGACTGGAAGCT770320.5728365477927216No Hit
TATTGCACTTGTCCCGGCCTGTA687990.511613117296597No Hit
ACTGGACTTGGAGTCAGAAGGC569070.42318009950722313No Hit
TGTCAGTTTGTCAAATACCCCA564950.4201163252615772No Hit
TGTAAACATCCTACACTCTCAGCT551620.41020367703476623No Hit
CATTGCACTTGTCTCGGTCTGA534490.3974652176105149No Hit
TTCTCACTACTGCACTTGACTAG508090.37783326613356No Hit
CCCATAAAGTAGAAAGCACTAC491240.3653030243764885No Hit
CAACGGAATCCCAAAAGCAGCTGT479750.35675866367685927No Hit
TTATAAAGCAATGAGACTGATT460890.3427337165232468No Hit
TTAATGCTAATCGTGATAGGGGTT442450.3290210958704042No Hit
TGAGGTAGTAGTTTGTGCTGTT428990.3190117751552597No Hit
CGCCTGTCTGAGCGTCGCT420350.3125867728537109No Hit
TAGCTTATCAGACTGATGTTGACA412100.3064517880171625No Hit
CAAAGAATTCTCCTTTTGGGCT407710.3031872324495931No Hit
TTCACAGTGGCTAAGTTCCGC366500.27254205364787687No Hit
CAACACCAGTCGATGGGCTGT350130.2603687564631136No Hit
CTTCTCACTACTGCACTTGACTAG348930.2594763950323429No Hit
TAGCAGCACGTAAATATTGGC315560.23466131091166173No Hit
AACCGGGCGGAAACACCA314470.2338507492787117No Hit
TTCAAGTAATCCAGGATAGGCTT307760.22886096161165234No Hit
CCCATAAAGTAGAAAGCACT304320.2263028588434431No Hit
TTCACAGTGGCTAAGTTCCG296340.22036865532881808No Hit
CAAAGAATTCTCCTTTTGGGCTT293520.21827160596650702No Hit
CTACGCCTGTCTGAGCGTCGCT285700.21245638397598474No Hit
TGTAAACATCCTACACTCAGCT277050.2060239453291795No Hit
AACATTCAACGCTGTCGGTGAGTT264120.1964087509126255No Hit
CCCCCACTGCTAAATTTGACTGGCTT256570.19079431024402663No Hit
CACCCGGCTGTGTGCACATGTGC250870.18655559344786596No Hit
TGAGAACTGAATTCCATAGGCTGT241800.1798108283002909No Hit
TGTCAGTTTGTCAAATACCCC240110.17855408595195554No Hit
TCGTACGACTCTTAGCGG239210.17788481487887756No Hit
CTCCCACTGCTTCACTTGACTAG234290.1742261330127178No Hit
TTCAAGTAATCCAGGATAGGC226530.1684555290937341No Hit
TGAGGTAGTAAGTTGTATTGTT221680.16484890164436927No Hit
TAGCAGCACGTAAATATTGGCGT219030.1628782701514174No Hit
AACGGAATCCCAAAAGCAGCTG213010.15840159030705117No Hit
TGTAAACATCCCCGACTGGAAGC212460.15799259131794793No Hit
TACGCCTGTCTGAGCGTCGCT211290.1571225389229465No Hit
CAACGGAATCCCAAAAGCAGCT209750.15597734175345748No Hit
TGAGGTAGTAGGTTGTATAGT209070.15547167027602077No Hit
AGCAGCATTGTACAGGGCTATGT205110.15252687755447755No Hit
CTCCCACTGCTTCACTTGACTAGCCT198520.14762632603049525No Hit
TTCACAGTGGCTAAGTTCTGC197860.1471355272435714No Hit
TGTCAGTTTGTCAAATACC195940.1457077489543383No Hit
ACCCCCCACTGCTAAATTTGACTG191420.14234652089843544No Hit
TGTAAACATCCTTGACTGGAAGC179780.1336906150199599No Hit
GGAATACCGGGTGCTGTAGGCTTT178640.13284287166072775No Hit
TCAGTGCACTACAGAACTTTGT178000.13236694556431672No Hit
TGAGAACTGAATTCCATGGGTT177970.13234463652854747No Hit
TATTGCACTTGTCCCGGCCTGTT177600.13206949175405983No Hit
ATCACATTGCCAGGGATTTCC175710.13066402250059603No Hit
CCCCCCACTGCTAAATTTGACTGG174410.12969729761726112No Hit
CACTAGATTGTGAGCTCCTGGA173730.1291916261398244No Hit
GCCTGTCTGAGCGTCGCT171220.12732510348046241No Hit
TGTCAGTTTGTCAAATACCC171100.12723586733738534No Hit
TTCACAGTGGCTAAGTTCTG170670.1269161044913592No Hit
CCCCCCACTGCTAAATTTGACTGGCT168990.12566679848828025No Hit
GTGGGCACATTACCCGTCTGACC167580.12461827380712472No Hit
TGAGGTAGTAGATTGTATAGT165850.12333178607776366No Hit
CAGTGCAATAGTATTGTCAAAGC163820.12182220799070993No Hit
ACTGGACTTGGAGTCAGAAGGCA162600.1209149738694264No Hit
TAGTGGGTTATCAGAACT159640.1187138156735254No Hit
TATTGCACTTGTCCCGGCCTGTAG154720.11505513380736564No Hit
ATCACATTGCCAGGGATTTCCA153520.11416277237659496No Hit
ACTGGACTTGGAGTCAGAAGGCT153290.11399173643569727No Hit
TGATGTAGTAGGTTGTATAGTT151920.1129729571355674No Hit
TAGCAGCACATAATGGTTTGT150520.11193186879966828No Hit
TAGCAGCACATCATGGTTTACA150250.11173108747774489No Hit
CAAAGTGCTGTTCGTGCAGGTAG147820.10992405558043426No Hit
CACTAGATTGTGAGCTCCTGGAA147810.10991661923517783No Hit
AGCAGCATTGTACAGGGCTATG144250.10726928032389152No Hit
ACTGGACTTGGAGTCAGAAGG143990.10707593534722452No Hit
TAGCTTATCAGACTGATGTTG142200.10574482954632494No Hit
AGAGGTAGTAGGTTGCATAGTT140480.1044657781622203No Hit
AGCTCGGTCTGAGGCCCCTCAGT139310.1035957257672189No Hit
AGAATTACTCTGAGACCT138290.10283721855106381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)