FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9919930
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9327689.402969577406292No Hit
CCCCCCACTGCTAAATTTGACTG6150606.20024536463463No Hit
TGAGGTAGTAGATTGTATAGTT4555384.592149339763487No Hit
TTCAAGTAATCCAGGATAGGCT4402074.43760187823906No Hit
CCCCCACTGCTAAATTTGACTG3401283.428733872114017No Hit
TAGCAGCACGTAAATATTGGCG2887872.9111798167930623No Hit
TAGCTTATCAGACTGATGTTGAC2599432.620411635969205No Hit
TAGCTTATCAGACTGATGTTGA2470992.490934915871382No Hit
AAGCTGCCAGTTGAAGAACTGT2295072.3135949548031087No Hit
TGAGGTAGTAGGTTGTATAGTT2035322.0517483490306887No Hit
TCGTACGACTCTTAGCGG1876421.8915657670971469No Hit
AACATTCAACGCTGTCGGTGAG1848301.8632187928745463No Hit
TGAGGTAGTAGTTTGTACAGTT1440101.451723953697254No Hit
TAGCTTATCAGACTGATGTTGACT1430371.441915416741852No Hit
TTCAAGTAATTCAGGATAGGTT1364231.375241559164228No Hit
TGAGAACTGAATTCCATGGGTT1351201.3621063858313516No Hit
CATAAAGTAGAAAGCACTACT1146101.1553508946131676No Hit
TATTGCACTTGTCCCGGCCTGT1040171.0485658668962383No Hit
TGTAAACATCCTTGACTGGAAGCT855710.8626169741117126No Hit
AACCGGGCGGAAACACCA764040.7702070478319907No Hit
TATTGCACTTGTCCCGGCCTGTAA759590.7657211290805479No Hit
TATTGCACTTGTCCCGGCCTGTAT733430.7393499752518415No Hit
TGAGAACTGAATTCCATAGGCTGT731640.737545527034969No Hit
AACATTCAACGCTGTCGGTGAGT663250.6686035082908851No Hit
TTAATGCTAATCGTGATAGGGGTT538540.5428868953712375No Hit
CATTGCACTTGTCTCGGTCTGA525150.5293888162517276No Hit
TGAGGTAGTAGATTGTATAGT502860.5069188996293321No Hit
TGATGTAGTAGATTGTATAGTT500080.5041164604992172No Hit
TTCACAGTGGCTAAGTTCCGC487630.49156596871147273No Hit
TATTGCACTTGTCCCGGCCTGTA463910.4676545096588383No Hit
CAACGGAATCCCAAAAGCAGCTGT455890.45956977518994585No Hit
AGCAGCATTGTACAGGGCTATGA442240.4458095974467562No Hit
TTCTCACTACTGCACTTGACTAG419900.42328927724288373No Hit
TGAGGTAGTAAGTTGTATTGTT356790.359669876702759No Hit
CACCCGGCTGTGTGCACATGTGC348080.3508895728094855No Hit
CAACGGAATCCCAAAAGCAGCT335820.33853061463135325No Hit
TGTAAACATCCCCGACTGGAAGCT319930.322512356437999No Hit
TGAGGTAGTAGTTTGTGCTGTT276290.27852011052497344No Hit
TAGCAGCACGTAAATATTGGC270730.2729152322647438No Hit
TAGCTTATCAGACTGATGTTGACA263430.2655563093691185No Hit
ACTGGACTTGGAGTCAGAAGGC255550.25761270492836136No Hit
CAACACCAGTCGATGGGCTGT251150.2531771897583955No Hit
AACGGAATCCCAAAAGCAGCTG245510.24749166576780282No Hit
CAAAGAATTCTCCTTTTGGGCT242950.24491100239618624No Hit
TCGTTTCCCGGCCAATGCACCA237270.23918515554041206No Hit
TGTCAGTTTGTCAAATACCCCA231110.23297543430245982No Hit
CCCATAAAGTAGAAAGCACTAC226540.22836854695547246No Hit
TGTAAACATCCTACACTCTCAGCT224220.226029820774945No Hit
TTCACAGTGGCTAAGTTCTGC223690.22549554281129No Hit
TGATGTAGTAGGTTGTATAGTT222700.22449755189804768No Hit
TTATAAAGCAATGAGACTGATT213220.21494103285003022No Hit
CTTCTCACTACTGCACTTGACTAG205350.20700750912556842No Hit
TGAGGTAGTAGATTGTATAGTTA200670.20228973389933197No Hit
GCCTGTCTGAGCGTCGCT185390.18688639939999577No Hit
CTCCCACTGCTTCACTTGACTAG183870.18535413052309846No Hit
TGTAAACATCCCCGACTGGA181260.18272306357000503No Hit
TTCAAGTAATCCAGGATAGGC180760.18221902775523618No Hit
CCCATAAAGTAGAAAGCACT170040.17141249988659193No Hit
TGGCTCAGTTCAGCAGGAAC169610.17097902908589074No Hit
TTCACAGTGGCTAAGTTCTG168440.16979958527933162No Hit
CCCCCACTGCTAAATTTGACTGGCTT165020.16635198030631265No Hit
TGAGAACTGAATTCCATGGGTTG160500.1617954965408022No Hit
TTCAAGTAATCCAGGATAGGCTT158970.1602531469476095No Hit
TGATGTAGTAGTTTGTACAGTT155930.15718860919381486No Hit
CAAAGAATTCTCCTTTTGGGCTT148460.1496583141211682No Hit
ACCCCCCACTGCTAAATTTGACTG146100.14727926507545921No Hit
TGAGGTAGTAGGTTGTATAGT143450.14460787525718427No Hit
TTCACAGTGGCTAAGTTCCG143290.14444658379645825No Hit
TATTGCACTTGTCCCGGCCTGTT142180.14332762428767137No Hit
TAGCTTATCAGACTGATGTTG138280.13939614493247432No Hit
CACCCGTAGAACCGACCTTGCG135790.13688604657492542No Hit
TGATAACTGAATTCCATGGGTT135190.1362812035972028No Hit
TGAGGTAGTAGTTTGTGCTGTTA135040.13612999285277216No Hit
GGAATACCGGGTGCTGTAGGCTTT129540.13058559889031476No Hit
TCAGTGCACTACAGAACTTTGT129370.13041422671329336No Hit
TGTCAGTTTGTCAAATACC125270.12628113303218874No Hit
CAAAGTGCTGTTCGTGCAGGTAG124850.1258577429477829No Hit
ATCACATTGCCAGGGATTTCC123500.124496846247907No Hit
TTCAAGTAATTCAGGATAGGTTA121860.12284360877546514No Hit
CACTAGATTGTGAGCTCCTGGA121500.12248070298883158No Hit
TGTCAGTTTGTCAAATACCCC119700.1206661740556637No Hit
ACCGGGTGCTGTAGGCTT114090.11501089221395715No Hit
AGCTCGGTCTGAGGCCCCTCAGT111760.11266208531713429No Hit
TAGCAGCACATCATGGTTTACA111760.11266208531713429No Hit
AATGCACCCGGGCAAGGATTCT108390.10926488392559222No Hit
AGCTACATTGTCTGCTGGGTTT108150.10902294673450316No Hit
TCGTTTCCCGGCCAACGCACCA108070.10894230100414015No Hit
CAAAGAATTCTCCTTTTGGGC107870.1087406866782326No Hit
TGTCAGTTTGTCAAATACCCCAA107810.10868020238046035No Hit
TGAGGTAGTAGTTTGTACAGT107630.10849874948714357No Hit
GGAATACCGGGTGCTGTAGGCTT106050.10690599631247397No Hit
CTCCCACTGCTTCACTTGACTAGCCT103910.1047487230252633No Hit
TAGCAGCACGTAAATATTGGCGT102860.1036902478142487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)