FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10580724
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG124373711.754743815262547No Hit
CAACGGAATCCCAAAAGCAGCTG9112528.612378510204028No Hit
CCCCCACTGCTAAATTTGACTG4186513.956733017513735No Hit
TTCAAGTAATCCAGGATAGGCT3873963.661337352718018No Hit
TAGCAGCACGTAAATATTGGCG3355483.1713141747199907No Hit
AAGCTGCCAGTTGAAGAACTGT2730352.5804944916812875No Hit
AACATTCAACGCTGTCGGTGAGT2598532.455909444382067No Hit
AACATTCAACGCTGTCGGTGAG1813581.714041496593239No Hit
TAGCTTATCAGACTGATGTTGAC1027540.9711433735536434No Hit
CATAAAGTAGAAAGCACTACT960910.9081703671695812No Hit
TATTGCACTTGTCCCGGCCTGTAT929420.8784086986864037No Hit
TATTGCACTTGTCCCGGCCTGTAA915220.8649880669791594No Hit
TAGCTTATCAGACTGATGTTGA902350.8528244381008332No Hit
TATTGCACTTGTCCCGGCCTGT843520.7972233280066657No Hit
TCGTACGACTCTTAGCGG827640.7822149032523672No Hit
TAGCTTATCAGACTGATGTTGACT790340.7469621171481271No Hit
TGAGGTAGTAGGTTGTATAGTT687740.6499933274887427No Hit
TTAATGCTAATCGTGATAGGGGTT654690.6187572797475863No Hit
CCCATAAAGTAGAAAGCACTAC567990.5368158171406796No Hit
TTCAAGTAATTCAGGATAGGTT557870.5272512542619957No Hit
GCCTGTCTGAGCGTCGCT548480.5183766252668532No Hit
AGCAGCATTGTACAGGGCTATGA545830.5158720707581068No Hit
TATTGCACTTGTCCCGGCCTGTA544670.5147757374637123No Hit
CAACGGAATCCCAAAAGCAGCTGT544340.5144638495437552No Hit
TGTAAACATCCTTGACTGGAAGCT511720.48363420121345196No Hit
CCCATAAAGTAGAAAGCACT502510.4749296929019224No Hit
AACCGGGCGGAAACACCA487220.46047888594391084No Hit
ATCCCGGACGAGCCCCCA452510.4276739474538794No Hit
ACTGGACTTGGAGTCAGAAGGC440120.4159639737318543No Hit
CATTGCACTTGTCTCGGTCTGA432440.40870549123103483No Hit
CAACGGAATCCCAAAAGCAGCT407770.3853895064269704No Hit
TGAGGTAGTAGATTGTATAGTT406120.383830066827185No Hit
TGTAAACATCCTACACTCTCAGCT368810.34856782957385524No Hit
CTCCCACTGCTTCACTTGACTAG352770.333408186434123No Hit
TTCTCACTACTGCACTTGACTAG339720.3210744368721838No Hit
TGTAAACATCCCCGACTGGAAGCT323680.30591479373245156No Hit
ACCGGGTGCTGTAGGCTT313450.29624626821378197No Hit
ACCGGGTGCTGTAGGCTTA307760.29086856438179465No Hit
CTTCTCACTACTGCACTTGACTAG279460.2641218124582023No Hit
CACTAGATTGTGAGCTCCTGGA279250.2639233383273205No Hit
CACCCGGCTGTGTGCACATGTGC270920.25605053113567655No Hit
TTCACAGTGGCTAAGTTCCG266690.2520526950707721No Hit
CGCCTGTCTGAGCGTCGCT260930.2466088331951575No Hit
AGCTCGGTCTGAGGCCCCTCAGT258230.24405702294096318No Hit
CAAAGAATTCTCCTTTTGGGCT252970.2390857185198291No Hit
GGAATACCGGGTGCTGTAGGCTTT251030.23725219559644503No Hit
ACCGGGTGCTGTAGGCTTT243900.23051352629555408No Hit
TGAGAACTGAATTCCATGGGTT239190.2260620350743484No Hit
AACGGAATCCCAAAAGCAGCTG236510.2235291271183333No Hit
CCCATAAAGTAGAAAGCACTA231890.21916269623893414No Hit
CAACACCAGTCGATGGGCTGT229070.2164974721956645No Hit
CTACGCCTGTCTGAGCGTCGCT225050.21269811026164184No Hit
ACCCCCCACTGCTAAATTTGACTG223990.21169628845814334No Hit
CACTAGATTGTGAGCTCCTGGAA222530.2103164206910605No Hit
TCGTTTCCCGGGCGGCGCACCA221120.20898380866942565No Hit
AACATTCAACGCTGTCGGTGAGTT210360.19881437224900678No Hit
GGAATACCGGGTGCTGTAGGCTT195480.18475106240366915No Hit
CCCCCACTGCTAAATTTGACTGGCTT193840.18320107395297336No Hit
CCCCCCACTGCTAAATTTGACTGG190210.17977030683344544No Hit
TTCAAGTAATCCAGGATAGGC185310.1751392437795372No Hit
TGAGGTAGTAGTTTGTACAGTT167420.1582311380582274No Hit
TAGCAGCACGTAAATATTGGC165600.1565110289239186No Hit
ATACCGGGTGCTGTAGGCTTA165480.15639761513484332No Hit
TACGCCTGTCTGAGCGTCGCT162240.15333544282981015No Hit
TGTCAGTTTGTCAAATACCCCAA159230.15049064695383793No Hit
CCCCCCACTGCTAAATTTGACTGGCT158890.15016930788479124No Hit
CCCCCCACTGCTAAATTTGACTGT158540.14983851766665496No Hit
CCCCCCACTGCTAAATTTGACTGA156660.14806170163780855No Hit
TATTGCACTTGTCCCGGCCTGTT155610.14706933098339964No Hit
TTCAAGTAATCCAGGATAGGCTT151990.14364801501296132No Hit
TGTCAGTTTGTCAAATACCCCA141860.13407400098518776No Hit
ATACCGGGTGCTGTAGGCTTT138600.13099292638197535No Hit
CAAAGAATTCTCCTTTTGGGCTT134380.12700454146616055No Hit
TGAGGTAGTAGTTTGTGCTGTT133910.12656033745894893No Hit
AGCTACATCTGGCTACTGGGTCTC133220.12590820817176596No Hit
TCGTTTCCCGGCCAATGCACCA130280.12312957033942101No Hit
ATACCGGGTGCTGTAGGCTT127790.12077623421610846No Hit
TCCTGTCTGAGCGTCGCT127040.1200673980343878No Hit
TATTGCACTTGTCCCGGCCTGTAG123650.1168634584930105No Hit
AGCTACATTGTCTGCTGGGTTT122940.11619242690764828No Hit
TGTAAACATCCTACACTCAGCT122000.11530401889322509No Hit
ACGCCTGTCTGAGCGTCGCT120910.11427384364245774No Hit
ACTGGACTTGGAGTCAGAAGG119720.1131491569007943No Hit
TGTAAACATCCCCGACTGGAAGC117170.1107391138829441No Hit
AATGACACGATCACTCCCGTTG116880.11046503055934546No Hit
GACCCGCGGGCGCTCTCCAGTCCT111180.10507787557826856No Hit
TTCACAGTGGCTAAGTTCTG110000.10396263998569473No Hit
TCAGTGCACTACAGAACTTTGT109850.1038208727493506No Hit
ACCACAGGGTAGAACCACGGAC108070.10213856821140027No Hit
AGCAGCATTGTACAGGGCTATGT107940.10201570327323538No Hit
AGCAGCATTGTACAGGGCTATG107220.10133522053878355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)